Mitochondrial and cytosolic calcium in rat hearts under high-K+ cardioplegia and pyruvate: Mechano-energetic performance

Canadian Journal of Physiology and Pharmacology

Volumn 89, Issue 7, 2011, Pages 485-496

  Consolini, A E ^a,b   Ragone, M I ^a   Bonazzola, P ^c  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b HOSPITAL UNIVERSITARI VALL D HEBRON   (Spain)

^c UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Ca2+; Calorimetry; Cardiomyocytes; Cardioplegia; Fura 2; Heart; Pyruvate; Rhod 2

Indexed keywords

2 [2 [4 (4 NITROBENZYLOXY)PHENYL]ETHYL]ISOTHIOUREA METHANESULFONATE; CALCIUM; FURA 2; POTASSIUM; PYRUVIC ACID; RHODAMINE; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; THAPSIGARGIN; 2 (2 (4 (4 NITROBENZYLOXY)PHENYL)ETHYL)ISOTHIOUREA METHANESULFONATE; 2-(2-(4-(4-NITROBENZYLOXY)PHENYL)ETHYL)ISOTHIOUREA METHANESULFONATE; DRUG DERIVATIVE; FUSED HETEROCYCLIC RINGS; RHOD 2; RHOD-2; THIOUREA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CALORIMETRY; CARDIOPLEGIA; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVITY; ENZYME INHIBITION; EXPERIMENTAL RAT; FLUORESCENCE; HEART MUSCLE CELL; HEART PERFORMANCE; HEART PERFUSION; HEART PROTECTION; ISOLATED HEART; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRIAL RESPIRATION; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; RAT; SARCOPLASMIC RETICULUM; ANIMAL; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; HEART; HEART ARREST; HEART MITOCHONDRION; HEART MUSCLE; METABOLISM; METHODOLOGY; WISTAR RAT;

ANIMALS; CALCIUM; CYTOSOL; FURA-2; HEART; HEART ARREST, INDUCED; HETEROCYCLIC COMPOUNDS, 3-RING; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA, HEART; MYOCARDIUM; POTASSIUM; PYRUVIC ACID; RATS; RATS, WISTAR; SARCOPLASMIC RETICULUM; SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES; THAPSIGARGIN; THIOUREA;

EID: 84856377929     PISSN: 00084212     EISSN: 12057541     Source Type: Journal    
DOI: 10.1139/Y11-042     Document Type: Article

References (45)

1. Bell, C.J., Bright, N.A., Rutter, G.A., and Griffiths, E.J. 2006. ATP regulation in adult rat cardiomyocytes: time-resolved decoding of rapid mitochondrial calcium spiking imaged with targeted photoproteins. J. Biol. Chem. 281(38): 28058-28067. doi:10.1074/jbc.M604540200. PMID:16882672.
2. Bers, D.M. 2001. Excitation-contraction coupling and cardiac contractile force. 2nd. ed. Kluwer Academic Publishers, Dordrecht, Netherlands.
3. Bünger, R., Mallet, R.T., and Hartman, D.A. 1989. Pyruvate-enhanced phosphorylation potential and inotropsim in normoxic and postischemic isolated working heart. Near-complete prevention of reperfusion contractile failure. Eur. J. Biochem. 180(1): 221-233. doi:10.1111/j.1432-1033.1989.tb14637.x. PMID:2707262.
4. Consolini, A.E., Márquez, M.T., and Ponce-Hornos, J.E. 1997. Energetics of heart muscle contraction under high K perfusion: verapamil and Ca effects. Am. J. Physiol.273: H2343-H2350.
5. Consolini, A.E., Quiroga, P., Yuln, G., and Volonté, M.G. 2004. Participation of Na/Ca-exchanger and sarcoplasmic reticulum in the high [K]o-protection against ischaemia-reperfusion dysfunction in rat hearts. Acta Physiol. Scand. 182(2): 121-132. doi:10.1111/j. 1365-201X.2004.01342.x. PMID:15450108.
6. Consolini, A.E., Ragone, M.I., Conforti, P., and Volonté, M.G. 2007. Mitochondrial role in ischemia-reperfusion of rat hearts exposed to high K cadioplegia and clonazepam: energetic and contractile consequences. Can. J. Physiol. Pharmacol. 85(5): 483-496. doi:10. 1139/Y07-022. PMID:17632582.
7. Cox, D.A., and Matlib, M.A. 1993. Modulation of intramitochondrial free Ca concentration by antagonists of Na/Ca exchange. Trends Pharmacol. Sci. 14(11): 408-413. doi:10.1016/0165-6147(93) 90063-P. PMID:8296399.
8. Das, A., Xi, L., and Kukreja, R.C. 2005. Phosphodiesterase-5 inhibitor sildenafil preconditions adult cardiac myocytes against necrosis and apoptosis. J. Biol. Chem. 280(13): 12944-12955. doi:10.1074/jbc.M404706200. PMID:15668244.
9. 2+ in mammalian myocardium. J. Gen. Physiol. 102(3): 575-597. doi:10.1085/jgp.102.3.575. PMID:8245824.
10. Griffiths, E.J. 2009. Mitochondrial calcium transport in the heart: physiological and pathological roles. J. Mol. Cell. Cardiol. 46(6): 789-803. doi:10.1016/j.yjmcc.2009.03.001. PMID:19285504.
11. Halestrap, A.P. 2009. What is the mitochondrial permeability transition pore? J. Mol. Cell. Cardiol. 46(6): 821-831. doi:10. 1016/j.yjmcc.2009.02.021. PMID:19265700.
12. Holroyd, S.M., Gibbs, C.L., and Wendt, I.R. 1990. The effect of increasing extracellular potassium concentration on the resting heat rate of the isolated rat papillary muscle. Pflugers Arch. 416(4): 406-412. doi:10.1007/BF00370747. PMID:2399114.
13. 2+ exchange in cells expressing NCX1. J. Biol. Chem. 271(37): 22391-22397. doi:10.1074/jbc.271.37.22391. PMID:8798401.
14. Knott, E.M., Sun, J., Lei, Y., Ryou, M.-G., Olivencia-Yurvati, A.H., and Mallet, R.T. 2006. Pyruvate mitigates oxidative stress during reperfusion of cardioplegia-arrested myocardium. Ann. Thorac. Surg. 81(3): 928-934. doi:10.1016/j.athoracsur.2005.08.046. PMID:16488697.
15. Kuznetsov, A.V., Troppmair, J., Sucher, R., Hermann, M., Saks, V., and Margreiter, R. 2006. Mitochondrial subpopulations and heterogeneity revealed by confocal imaging: possible physiological role? Biochim. Biophys. Acta, 1757(5/6): 686-691. PMID:16712778.
16. Langer, G.A. 1997. Excitation-contraction coupling and calcium compartmentation. In The myocardium. Edited by G.A. Langer. Academic Press, San Diego, Calif. pp. 181-233.
17. Loiselle, D.S. 1987. Cardiac basal and activation metabolism. Basic Res. Cardiol. 82(Suppl. 2): 37-50. PMID:2821985.
18. Márquez, M.T., Consolini, A.E., Bonazzola, P., and Ponce-Hornos, J. E. 1997. The energetics of the quiescent heart muscle: high potassium cardioplegic solution and the influence of calcium and hypoxia on the rat heart. Acta Physiol. Scand. 160(3): 229-233. oi:10.1046/j.1365-201X.1997.00137.x. PMID:9246385.
19. Martin, B.J., Valdivia, H.H., Bünger, R., Lasley, R.D., and Mentzer, R.M., Jr. 1998. Pyruvate augments calcium transients and cell shortening in rat ventricular myocytes. Am. J. Physiol. 274(1 Pt. 2): H8-H17. PMID:9458846.
20. Mei, Y., Liu, H., Long, C., Cheng, B., Gao, S., and Hu, D. 2006. Effect of four crystalloid cardioplegias on immature rabbit hearts during global ischemia. Asian J. Surg. 29(2): 79-85. doi:10.1016/ S1015-9584(09)60112-3. PMID:16644507.
21. Mulieri, L.A., and Alpert, N.R. 1982. Activation heat and latency relaxation in relation to calcium movement in skeletal and cardiac muscle. Can. J. Physiol. Pharmacol. 60(4): 529-541. doi:10.1139/ y82-073. PMID:6286074.
22. NIH. 1996 (revised). Guide for the care and use of laboratory animals. US National Institutes of Health. Publication No. 85-23, National Academy Press, Washington, D.C., Wash.
23. 2+ uptake: tortoise or hare? J. Mol. Cell. Cardiol. 46(6): 767-774. doi:10. 1016/j.yjmcc.2008.12.011. PMID:19162034.
24. Olivencia-Yurvati, A.H., Blair, J.L., Baig, M., and Mallet, R.T. 2003. Pyruvate-enhanced cardioprotection during surgery with cardiopulmonary bypass. J. Cardiothorac. Vasc. Anesth. 17(6): 715-720. doi:10.1053/j.jvca.2003.09.007. PMID:14689411.
25. Ponce-Hornos, J.E. 1990. Energetics of calcium movements. In Calcium and the heart. Edited by G.A. Langer. Raven Press Ltd., New York, N.Y. Chapter 8, pp.269-298.
26. Ponce-Hornos, J.E., Márquez, M.T., and Bonazzola, P. 1992. Influence of extracellular potassium on energetics of resting heart muscle. Am. J. Physiol. 262(4 Pt. 2): H1081-H1087. PMID: 1566890.
27. Ponce-Hornos, J.E., Bonazzola, P., Marengo, F.D., Consolini, A.E., and Márquez, M.T. 1995. Tension-dependent and tensionindependent energy components of heart contraction. Pflugers Arch. 429(6): 841-851. doi:10.1007/BF00374809. PMID: 7603838.
28. Powell, T., Tatham, P.E.R., and Twist, V.W. 1984. Cytoplasmic free calcium measured by quin-2 fluorescence in isolated ventricular myocytes at rest and during potassium-depolarization. Biochem. Biophys. Res. Commun. 122(3): 1012-1020. doi:10.1016/0006291X(84)91192-6. PMID:6477545.
29. +]o cardioprotection of rat hearts under ischemia and reperfusion: A mechano-energetic study. J. Cardiovasc. Pharmacol. 54(3): 213-222. doi:10.1097/FJC. 0b013e3181b04ce3. PMID:19597370.
30. Rathi, S.S., Saini, H.K., Xu, Y.-J., and Dhalla, N.S. 2004. Mechanisms of low Na+-induced increase in intracellular calcium in KCl-depolarized rat cardiomyocytes. Mol. Cell. Biochem. 263(1-2): 151-162. doi:10.1023/B:MCBI.0000041857.86178.f6. PMID: 15524176.
31. Rizzuto, R., Bernardi, P., and Pozzan, T. 2000. Mitochondria as allround players of the calcium game. J. Physiol. 529(1): 37-47. doi:10.1111/j.1469-7793.2000.00037.x. PMID:11080249.
32. Ruiz-Meana, M., García-Dorado, D., Hofstaetter, B., Piper, H.M., and Soler-Soler, J. 1999. Propagation of cardiomyocyte hypercontracture by passage of Na+ through gap junctions. Circ. Res. 85(3): 280-287. PMID:10436171.
33. 2+ overloaded cardiac myocytes. Basic Res. Cardiol. 102(6): 542-552. doi:10.1007/s00395-0070675-y. PMID:17891523.
34. Ruiz-Meana, M., Abellán, A., Miró-Casas, E., Agulló, E., and García-Dorado, D. 2009. Role of sarcoplasmic reticulum in mitochondrial permeability transition and cardiomyocyte death during reperfusion. Am. J. Physiol. Heart Circ. Physiol. 297(4): H1281-H1289. doi:10.1152/ajpheart.00435.2009. PMID: 19684187.
35. 2+ from the endoplasmic reticulum to mitochondria: is spatial intimacy enough? Biochem. Soc. Trans. 34(3): 351-355. doi:10.1042/BST0340351. PMID:16709159.
36. Salvioli, S., Ardizzoni, A., Franceschi, C., and Cossarizza, A. 1997. JC-1 but not DiOC6(3) or rhodamine 123 is a reliable fluorescent probe to asses Dψm changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411(1): 77-82. doi:10.1016/S0014-5793(97)00669-8. PMID: 9247146.
37. 2+ exchange inhibitor KB-R7943 is also a potent inhibitor of the mitochondrial Ca+2 uniporter. Br. J. Pharmacol. 151(5): 647-654. doi:10.1038/sj.bjp.0707260. PMID: 17471180.
38. 2+ concentration during depolarization in heart muscle. Am. J. Physiol. 250(4): C651-C656. PMID:3963177.
39. Siess, M. 1987. Cardiac energetics: significance of mitochondria. In Cardiac energetics: basic mechanisms and clinical implications. Edited by R. Jacob, H.J. Just, and C.H. Holubarsch. Springer, New York, N.Y. pp. 69-83.
40. Sperelakis, N., and Schneider, J.A. 1976. Metabolic control mechanism for calcium ion influx that may protect the ventricular myocardial cell. Am. J. Cardiol. 37(7): 1079-1085. doi:10.1016/ 0002-9149(76)90428-8. PMID:1274870.
41. Stowe, D.F., Varadarajan, S.G., An, J., and Smart, S.C. 2000. Reduced cytosolic Ca loading and improved cardiac function after cardioplegic cold storage of guinea-pig isolated hearts. Circulation, 102(10): 1172-1177. PMID:10973848.
42. Tejero-Taldo, M.I., Sun, J., Caffrey, J.I., and Mallet, R.T. 1998. Pyruvate potentiates b-adrenergic inotropism of stunned guineapig myocardium. J. Mol. Cell. Cardiol. 30(11): 2327-2339. doi:10. 1006/jmcc.1998.0792. PMID:9925369.
43. 2+ transients during the contractile cycle in adult rabbit cardiac myocytes. Biochem. Biophys. Res. Commun. 236(3): 738-742. doi:10.1006/bbrc.1997.7042. PMID:9245725.
44. Wang, J., Zhou, X., Zhao, Y., Tang, H., and Hu, Y. 2003. Myocardial protection of immature rabbits: polarizing versus depolarizing. J. Surg. Res. 111(1): 1-7. doi:10.1016/S0022-4804(03)00051-9. PMID:12842441.
45. 2+ release in rats via mitochondria-dependent and-independent mechanisms. J. Physiol. 550(3): 765-783. doi:10.1113/jphysiol. 2003.040345. PMID:12824454.