Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae

Applied Microbiology and Biotechnology

Volumn 93, Issue 3, 2012, Pages 1157-1166

  Pisanelli, Ines ^a   Wührer, Petra ^a   Reyes Dominguez, Yazmid ^a   Spadiut, Oliver ^a,b   Haltrich, Dietmar ^a   Peterbauer, Clemens ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Aspergillus; Carbohydrate oxidoreductases; Electron acceptors; Hydrogen peroxide; Pyranose oxidase

Indexed keywords

1 ,4-BENZOQUINONE; 2 ,6-DICHLOROINDOPHENOL; ASPERGILLUS NIDULANS; ASPERGILLUS ORYZAE; BENZOQUINONES; BIOCHEMICAL CHARACTERIZATION; CATALYTIC EFFICIENCIES; CDNA ENCODING; CODING SEQUENCES; D-GLUCOSE; E. COLI; ELECTRON ACCEPTOR; ELECTRON ACCEPTORS; HETEROLOGOUS EXPRESSION; KINETIC CONSTANT; PURIFIED ENZYME; PYRANOSE; PYRANOSE OXIDASE; TWO-ELECTRON REDUCTION;

CARBOHYDRATES; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; GLUCOSE; GLUCOSE OXIDASE; HYDROGEN; HYDROGEN PEROXIDE; PURIFICATION;

ASPERGILLUS;

1,4 BENZOQUINONE; 2,6 DICHLOROPHENOLINDOPHENOL; FLAVOPROTEIN; FUNGAL ENZYME; GLUCOSE; OXIDOREDUCTASE; OXYGEN; PYRANOSE 2 OXIDASE; UNCLASSIFIED DRUG;

BIOCHEMICAL COMPOSITION; CATALYSIS; COLIFORM BACTERIUM; ENZYME ACTIVITY; FUNGUS; GENOME; HYDROGEN PEROXIDE; MITOCHONDRIAL DNA; SUBSTRATE;

ARTICLE; ASPERGILLUS NIDULANS; ASPERGILLUS ORYZAE; CATALYSIS; CONTROLLED STUDY; ELECTRON; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; ESCHERICHIA COLI; FUNGAL GENOME; GENETIC CODE; NONHUMAN; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ASPERGILLUS NIDULANS; ASPERGILLUS ORYZAE; BENZOQUINONES; CARBOHYDRATE DEHYDROGENASES; DNA, COMPLEMENTARY; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

ASCOMYCOTA; ASPERGILLUS; ASPERGILLUS ORYZAE; EMERICELLA NIDULANS; ESCHERICHIA COLI;

EID: 84856376930     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3568-9     Document Type: Article

References (33)

1. Akileswaran L, Brock B, Cereghino J, Gold M (1999) 1,4-Benzoquinone reductase from Phanerochaete chrysosporium: cDNA cloning and regulation of expression. Appl Environ Microbiol 65:415-21 (Pubitemid 29075077)
2. Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-402 (Pubitemid 27359211)
3. Ausubel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, Struhl K (1990) Current protocols in molecular biology. Wiley, New York
4. Bannwarth M, Bastian S, Heckmann-Pohl D, Giffhorn F, Schulz G (2004) Crystal structure of pyranose 2-oxidase from the white-rot fungus Peniophora sp. Biochemistry 43:11683-90 (Pubitemid 39277613)
5. Bayne S, Fewster JA (1956) The osones. Adv Carbohydr Chem 11:43-96
6. Bond CR, Knight EC, Walker TK (1937) The production of glucosone from carbohydrates by enzymic action. Biochem J 31:1033-40
7. Brock B, Gold M (1996) 1,4-Benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis. Arch Biochem Biophys 331:31-40 (Pubitemid 26230616)
8. 2 during wood degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida. Appl Environ Microbiol 60:2524-32 (Pubitemid 24205755)
9. de Koker T, Mozuch M, Cullen D, Gaskell J, Kersten P (2004) Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation. Appl Environ Microbiol 70:5794-800 (Pubitemid 39471819)
10. Dietrich D, Crooks C (2009) Gene cloning and heterologous expression of pyranose 2-oxidase from the brown-rot fungus, Gloeophyllum trabeum. Biotechnol Lett 31:1223-8
11. Forneris F, Orru R, Bonivento D, Chiarelli LR, Mattevi A (2009) ThermoFAD, a Thermofluor®-adapted flavin ad hoc detection system for protein folding and ligand binding. FEBS J 276:2833-40
12. Giffhorn F (2000) Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl Microbiol Biotechnol 54:727-40 (Pubitemid 32009732)
13. Hallberg B, Leitner C, Haltrich D, Divne C (2004) Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase. J Mol Biol 341:781-96 (Pubitemid 39118411)
14. Hammel K, Kapich A, Jensen K Jr, Ryan Z (2002) Reactive oxygen species as agents of wood decay by fungi. Enzym Microb Tech 30:445-53 (Pubitemid 34310268)
15. Johnstone IL, Hughes SG, Clutterbuck AJ (1985) Cloning an Aspergillus nidulans developmental gene by transformation. EMBO J 4:1307-11
16. Kim K-A, Fravel DR, Papavizas GC (1990) Glucose oxidase as the antifungal principle of talaron from Talaromyces flavus. Can J Microbiol 36:760-4
17. Kittl R, Sygmund C, Halada P, Volc J, Divne C, Haltrich D, Peterbauer CK (2008) Molecular cloning of three pyranose dehydrogenase-encoding genes from Agaricus meleagris and analysis of their expression by real-time RT-PCR. Curr Genet 53:117-27
18. 3 by pyranose 2-oxidase. J Biol Chem 281:35104-15 (Pubitemid 46036550)
19. Leiter E, Marx F, Pusztaheli T, Haas H, Pocsi I (2004) Penicillium chrysogenum glucose oxidase - A study on its antifungal effects. J Appl Microbiol 97:1201-9 (Pubitemid 39573944)
20. Leitner C, Volc J, Haltrich D (2001) Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor. Appl Environ Microbiol 67:3636-44 (Pubitemid 33641236)
21. Liu D, Coloe S, Baird R, Pederson J (2000) Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol 38:471 (Pubitemid 30043352)
22. Maresova H, Vecerek B, Hradska M, Libessart N, Becka S, Saniez M, Kyslik P (2005) Expression of the pyranose 2-oxidase from Trametes pubescens in Escherichia coli and characterization of the recombinant enzyme. J Biotechnol 120:387-95 (Pubitemid 41627968)
23. Martinez A, Speranza M, Ruiz-Duenas F, Ferreira P, Camarero S, Guillen F, Martinez M, Gutierrez A, Del Rio J (2005) Biodegradation of lignocellulosics: Microbial, chemical, and enzymatic aspects of the fungal attack of lignin. Int Microbiol 8:195-204 (Pubitemid 41322270)
24. Nishimura I, Okada K, Koyama Y (1996) Cloning and expression of pyranose oxidase cDNA from Coriolus versicolor in Escherichia coli. J Biotechnol 52:11-20 (Pubitemid 27064422)
25. Pisanelli I, Kujawa M, Spadiut O, Kittl R, Halada P, Volc J, Mozuch M, Kersten P, Haltrich D, Peterbauer CK (2009) Pyranose 2-oxidase from Phanerochaete chrysosporium-expression in E. coli and biochemical characterization. J Biotechnol 142:97-106
26. Porath J (1992) Immobilized metal ion affinity chromatography. Prot Expr Purif 3:263-81
27. Punt PJ, van den Hondel CAMJJ (1992) Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Meth Enzymol 216:447-57 (Pubitemid 23041089)
28. Reyes-Dominguez Y, Bok JW, Berger H, Keats Shwab E, Basheer A, Gallmetzer A, Scazzocchio C, Keller N, Strauss J (2010) Heterochromatic marks are associated with the repression of secondary metabolism clusters in Aspergillus nidulans. Mol Microbiol 76:1376-86
29. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
30. Takakura Y, Kuwata S (2003) Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete Tricholoma matsutake. Biosci Biotechnol Biochem 67:2598-607 (Pubitemid 39267368)
31. Tasca F, Timur S, Ludwig R, Haltrich D, Volc J, Antiochia R, Gorton L (2007) Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes. Electroanalysis 19:294-302
32. Vecerek B, Maresova H, Kocanova M, Kyslik P (2004) Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli. Appl Microbiol Biotechnol 64:525-30 (Pubitemid 38691117)
33. Volc J, Kubatova E, Daniel G, Prikrylova V (1996) Only C-2 specific glucose oxidase activity is expressed in ligninolytic cultures of the white rot fungus Phanerochaete chrysosporium. Arch Microbiol 165:421-4 (Pubitemid 126423397)