Detecting, visualising, and quantifying mucins

Methods in Molecular Biology

Volumn 842, Issue , 2012, Pages 49-66

  Harrop, Ceri A ^a   Thornton, David J ^a   McGuckin, Michael A ^b  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b MATER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

Antibody; Bronchial epithelial cell culture; Detection; ELISA; Immunohistochemistry; Lectin; Mucin; Western blotting

Indexed keywords

MUCIN;

ARTICLE; CELL CULTURE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITHELIUM CELL; HUMAN; NUCLEIC ACID HYBRIDIZATION; WESTERN BLOTTING;

BLOTTING, WESTERN; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITHELIAL CELLS; HUMANS; MUCINS; NUCLEIC ACID HYBRIDIZATION;

EID: 84856364677     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-513-8_3     Document Type: Article

References (23)

1. Hattrup, C. L., and Gendler, S. J. (2008) Structure and function of the cell surface (tethered) mucins. Annu Rev Physiol 70, 431-457.
2. Thornton, D. J., Rousseau, K., and McGuckin, M. A. (2008) Structure and function of the polymeric mucins in airways mucus. Annu Rev Physiol 70, 459-486.
3. Desseyn, J. L., Aubert, J. P., Van Seuningen, I., Porchet, N., and Laine, A. (1997) Genomic organization of the 3 ¢ region of the human mucin gene MUC5B. J Biol Chem 272, 16873-16883.
4. Desseyn, J. L., Buisine, M. P., Porchet, N., Aubert, J. P., and Laine, A. (1998) Genomic organization of the human mucin gene MUC5B. cDNA and genomic sequences upstream of the large central exon. J Biol Chem 273, 30157-30164.
5. Buisine, M. P., Desseyn, J. L., Porchet, N., Degand, P., Laine, A., and Aubert, J. P. (1998) Genomic organization of the 3 ¢ -region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family. Biochem J 332 (Pt 3), 729-738.
6. Desseyn, J. L., Guyonnet-Duperat, V., Porchet, N., Aubert, J. P., and Laine, A. (1997) Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family. J Biol Chem 272, 3168-3178.
7. Carlstedt, I., Lindgren, H., Sheehan, J. K., Ulmsten, U., and Wingerup, L. (1983) Isolation and characterization of human cervical-mucus glycoproteins. Biochem J 211, 13-22.
8. Carlstedt, I., and Sheehan, J. K. (1984) Is the macromolecular architecture of cervical, respiratory and gastric mucins the same? Biochem Soc Trans 12, 615-617.
9. Mantle, M., and Allen, A. (1978) A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. Biochem Soc Trans 6, 607-609.
10. Thornton, D. J., Howard, M., Devine, P. L., and Sheehan, J. K. (1995) Methods for separation and deglycosylation of mucin subunits. Anal Biochem 227, 162-167.
11. Gerken, T. A., Gupta, R., and Jentoft, N. (1992) A novel approach for chemically deglycosylating O-linked glycoproteins. The deglycosylation of submaxillary and respiratory mucins. Biochemistry 31, 639-648.
12. Raju, T. S., and Davidson, E. A. (1994) New approach towards deglycosylation of sialoglycoproteins and mucins. Biochem Mol Biol Int 34, 943-954.
13. Hong, J. C., and Kim, Y. S. (2000) Alkalicatalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections. Glycoconj J 17, 691-703.
14. Kim, Y. S., Gum, J., Jr., and Brockhausen, I. (1996) Mucin glycoproteins in neoplasia. Glycoconj J 13, 693-707.
15. Young, P. R. (1989) Enhancement of immunoblot staining using a mixed chromogenic substrate. J Immunol Methods 121, 295-296.
16. Randell, S. H., Walstad, L., Schwab, U. E., Grubb, B. R., and Yankaskas, J. R. (2001) Isolation and culture of airway epithelial cells from chronically infected human lungs. In Vitro Cell Dev Biol Anim 37, 480-489.
17. Holmen, J. M., Karlsson, N. G., Abdullah, L. H., Randell, S. H., Sheehan, J. K., Hansson, G. C., and Davis, C. W. (2004) Mucins and their O-Glycans from human bronchial epithelial cell cultures. Am J Physiol Lung Cell Mol Physiol 287, L824-L834.
18. Gray, T. E., Guzman, K., Davis, C. W., Abdullah, L. H., and Nettesheim, P. (1996) Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am J Respir Cell Mol Biol 14, 104-112.
19. Diamandis, E. P., Christopoulos, T.K. (1989) Immunoassay, Academic Press, San Diego .
20. McGuckin, M. A., Devine, P. L., Ramm, L. E., and Ward, B. G. (1994) Factors effecting the measurement of tumor-associated MUC1 mucins in serum. Tumour Biol 15, 33-44.
21. Thornton, D. J., Holmes, D. F., Sheehan, J. K., and Carlstedt, I. (1989) Quantitation of mucus glycoproteins blotted onto nitrocellulose membranes. Anal Biochem 182, 160-164.
22. Ayre, D., Hutton, D. A., and Pearson, J. P. (1994) The use of wheat germ agglutinin to improve binding of heterogeneous mucin species to nitrocellulose membranes. Anal Biochem 219, 373-375.
23. Jay, G. D., Culp, D. J., and Jahnke, M. R. (1990) Silver staining of extensively glycosylated proteins on sodium dodecyl sulfate-polyacrylamide gels: enhancement by carbohydratebinding dyes. Anal Biochem 185, 324-330.