Gene profiling of laser-microdissected brain regions and individual cells in drug abuse and schizophrenia research

Methods in Molecular Biology

Volumn 829, Issue , 2012, Pages 541-550

  Sanna, Pietro Paolo ^a   Repunte Canonigo, Vez ^a   Guidotti, Alessandro ^b  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF ILLINOIS   (United States)

Author keywords

Array; Expression; Gene profiling; Laser capture microdissection; Laser microdissection; Laser pressure catapulting; Microarray; mRNA

Indexed keywords

MESSENGER RNA; MICRORNA; TRANSCRIPTOME;

ADDICTION; ANIMAL; BRAIN; CYTOLOGY; DNA SEQUENCE; GENE EXPRESSION PROFILING; GENETICS; LASER; METABOLISM; METHODOLOGY; MICRODISSECTION; MOUSE; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; REVIEW; SCHIZOPHRENIA;

ANIMALS; BASE SEQUENCE; BRAIN; GENE EXPRESSION PROFILING; LASERS; MICE; MICRODISSECTION; MICRORNAS; RNA, MESSENGER; SCHIZOPHRENIA; SEQUENCE ANALYSIS, DNA; SUBSTANCE-RELATED DISORDERS; TRANSCRIPTOME;

EID: 84856337850     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-458-2_34     Document Type: Review

References (20)

1. Repunte-Canonigo, V.; van der Stap, L. D.; Chen, J.; Sabino, V.; Wagner, U.; Zorrilla, E. P.; Schumann, G.; Roberts, A. J.; Sanna, P. P. (2010) Genome-wide gene expression analysis identifi es K-ras as a regulator of alcohol intake, Brain Res 1339, 1-10.
2. Repunte-Canonigo, V.; Berton, F.; Cottone, P.; Reifel-Miller, A.; Roberts, A. J.; Morales, M.; Francesconi, W.; Sanna, P. P. (2010) A potential role for adiponectin receptor 2 (AdipoR2) in the regulation of alcohol intake, Brain Res 1339, 11-17.
3. Sanna, P. P.; King, A. R.; van der Stap, L. D.; Repunte-Canonigo, V. (2005) Gene profi ling of laser-microdissected brain regions and subregions, Brain Res Brain Res Protoc 15, 66-74. (Pubitemid 40828886)
4. Satta, R.; Maloku, E.; Zhubi, A.; Pibiri, F.; Hajos, M.; Costa, E.; Guidotti, A. (2008) Nicotine decreases DNA methyltransferase 1 expression and glutamic acid decarboxylase 67 promoter methylation in GABAergic interneurons, Proc Natl Acad Sci U S A 105, 16356-16361.
5. Ruzicka, W. B.; Zhubi, A.; Veldic, M.; Grayson, D. R.; Costa, E.; Guidotti, A. (2007) Selective epigenetic alteration of layer I GABAergic neurons isolated from prefrontal cortex of schizophrenia patients using laserassisted microdissection, Mol Psychiatry 12, 385-397. (Pubitemid 46496836)
6. Costa, E.; Auta, J.; Grayson, D. R.; Matsumoto, K.; Pappas, G. D.; Zhang, X.; Guidotti, A. (2002) GABAA receptors and benzodiazepines: a role for dendritic resident subunit mRNAs, Neuropharmacology 43, 925-937. (Pubitemid 35345145)
7. Cuello, A.; Carson, S. (1983) Microdisection of fresh rat brain tissue slices, in Brain microdissection techniques (Cuello, A.; Ed.), pp 37-126, John Wiley and Son, New York.
8. Palkovitis, M.; Brownstein, M. (1983) Microdissection of brain areas by the punch technique, in Brain microdissection techniques (Cuello, A.; Ed.), pp 1-36, John Wiley and Son.
9. Ginsberg, S. D.; Elarova, I.; Ruben, M.; Tan, F.; Counts, S. E.; Eberwine, J. H.; Trojanowski, J. Q.; Hemby, S. E.; Mufson, E. J.; Che, S. (2004) Single-cell gene expression analysis: implications for neurodegenerative and neuropsychiatric disorders, Neurochem Res 29, 1053-1064. (Pubitemid 39023481)
10. Goldsworthy, S. M.; Stockton, P. S.; Trempus, C. S.; Foley, J. F.; Maronpot, R. R. (1999) Effects of fi xation on RNA extraction and amplifi cation from laser capture microdissected tissue, Mol Carcinog 25, 86-91. (Pubitemid 29275020)
11. Specht, K.; Richter, T.; Muller, U.; Walch, A.; Werner, M.; Hofl er, H. (2001) Quantitative gene expression analysis in microdissected archival formalin-fi xed and paraffi n-embedded tumor tissue, Am J Pathol 158, 419-429. (Pubitemid 32158605)
12. Parlato, R.; Rosica, A.; Cuccurullo, V.; Mansi, L.; Macchia, P.; Owens, J. D.; Mushinski, J. F.; De Felice, M.; Bonner, R. F.; Di Lauro, R. (2002) A preservation method that allows recovery of intact RNA from tissues dissected by laser capture microdissection, Anal Biochem 300, 139-145. (Pubitemid 34093687)
13. Karsten, S. L.; Van Deerlin, V. M.; Sabatti, C.; Gill, L. H.; Geschwind, D. H. (2002) An evaluation of tyramide signal amplifi cation and archived fi xed and frozen tissue in microarray gene expression analysis, Nucleic Acids Res 30, E4.
14. Kamme, F.; Salunga, R.; Yu, J.; Tran, D. T.; Zhu, J.; Luo, L.; Bittner, A.; Guo, H. Q.; Miller, N.; Wan, J.; Erlander, M. (2003) Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity, J Neurosci 23, 3607-3615. (Pubitemid 36958468)
15. Benes, F. M.; Davidson, J.; Bird, E. D. (1986) Quantitative cytoarchitectural studies of the cerebral cortex of schizophrenics, Arch Gen Psychiatry 43, 31-35. (Pubitemid 16148968)
16. Selemon, L. D.; Rajkowska, G.; Goldman- Rakic, P. S. (1998) Elevated neuronal density in prefrontal area 46 in brains from schizophrenic patients: application of a three-dimensional, stereologic counting method, J Comp Neurol 392, 402-412. (Pubitemid 28165894)
17. Simunovic, F.; Yi, M.; Wang, Y.; Macey, L.; Brown, L. T.; Krichevsky, A. M.;ersen, S. L.; Stephens, R. M.; Benes, F. M.; Sonntag, K. C. (2009) Gene expression profi ling of substantia nigra dopamine neurons: further insights into Parkinsons disease pathology, Brain 132, 1795-1809.
18. Arcturus. (2004) Application note #1. Optimized protocol for preparing and staining LCM samples from frozen tissue and extraction of high quality RNA, http://www.arctur. com/.
19. Xiang, C. C.; Mezey, E.; Chen, M.; Key, S.; Ma, L.; Brownstein, M. J. (2004) Using DSP, a reversible cross-linker, to fi x tissue sections for immunostaining, microdissection and expression profi ling, Nucleic Acids Res 32, e185.
20. Burbach, G. J.; Dehn, D.; Nagel, B.; Del Turco, D.; Deller, T. (2004) Laser microdissection of immunolabeled astrocytes allows quantifi cation of astrocytic gene expression, J Neurosci Methods 138, 141-148. (Pubitemid 39489842)