Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses

Applied Microbiology and Biotechnology

Volumn 93, Issue 2, 2012, Pages 679-685

  Zhou, Yong Hong ^a   Zhang, Yong Jun ^a   Luo, Zhi Bing ^a   Fan, Yan Hua ^a   Tang, Gui Rong ^a   Liu, Li Ji ^a   Pei, Yan ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Beauveria bassiana; Quantitative PCR; Reference genes; Selection

Indexed keywords

ABIOTIC STRESS; BEAUVERIA BASSIANA; DEVELOPMENT STAGES; ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA; EXPRESSED GENES; EXPRESSION ANALYSIS; NUTRIENT CONDITIONS; QUANTITATIVE PCR; QUANTITATIVE POLYMERASE CHAIN REACTION; SELECTION; STRESS CONDITION;

POLYMERASE CHAIN REACTION;

GENE EXPRESSION;

ACTIN; BETA TUBULIN; CYCLOPHILIN B; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTONE H2A; HISTONE H3; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; PROTEIN TYROSINE PHOSPHATASE SHP 1; RIBOSOME RNA; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR; TRANSLATION ELONGATION FACTOR; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ENTOMOPATHOGENIC ORGANISM; FUNGUS; GENE EXPRESSION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

ABIOTIC STRESS; ARTICLE; BEAUVERIA BASSIANA; COPY NUMBER VARIATION; ENTOMOPATHOGENIC FUNGUS; ENVIRONMENTAL STRESS; ENZYME ANALYSIS; ENZYME STABILITY; GENE EXPRESSION; MYCOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; NUTRIENT; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION;

ANTIFUNGAL AGENTS; BEAUVERIA; CULTURE MEDIA; DNA, FUNGAL; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, FUNGAL; MOLECULAR SEQUENCE DATA; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SEQUENCE ANALYSIS, DNA;

CORDYCEPS BASSIANA; FUNGI;

EID: 84856300307     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3561-3     Document Type: Article

References (24)

1. Andersen CL, Jensen JL, ∅rntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64(15):5245-5250 (Pubitemid 39006544)
2. Bustin S (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29(1):23-29 (Pubitemid 34965744)
3. Bustin S, Benes V, Nolan T, Pfaffl M (2005) Quantitative real-time RT-PCR-a perspective. J Mol Endocrinol 34(3):597-601 (Pubitemid 40903586)
4. Cho EM, Kirkland BH, Holder DJ, Keyhani NO (2007) Phage display cDNA cloning and expression analysis of hydrophobins from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. Microbiology 153(10):3438-3447 (Pubitemid 47629548)
5. Clarkson JM, Charnley AK (1996) New insights into the mechanisms of fungal pathogenesis in insects. Trends Microbiol 4(5):197-203 (Pubitemid 26163468)
6. de Almeida MR, Ruedell CM, Ricachenevsky FK, Sperotto RA, Pasquali G, Fett-Neto AG (2010) Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill. BMC Mol Biol 11:73
7. Dheda K, Huggett J, Chang J, Kim L, Bustin S, Johnson M, Rook G, Zumla A (2005) The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344(1):141-143 (Pubitemid 41569389)
8. Fan Y, Fang W, Guo S, Pei X, Zhang Y, Xiao Y, Li D, Jin K, Bidochka MJ, Pei Y (2006) Increased insect virulence in Beauveria bassiana overexpressing an engineered chitinase. Appl Environ Microb 73(1):295-302 (Pubitemid 46079962)
9. Fang W, Zhang Y, Xiao Y, Ma J, Pei Y (2003) Cloning and characterization of the promoter of subtilisin-like protease gene CDEP-1 from Beauveria bassiana. Mycosystema 22(2):252-258
10. Foldager CB, Munir S, Ulrik-Vinther M, Søballe K, Bünger C, Lind M (2009) Validation of suitable house keeping genes for hypoxia-cultured human chondrocytes. BMC Mol Biol 10:94
11. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6(4):279-284 (Pubitemid 40903399)
12. Jin K, Zhang Y, Luo Z, Xiao Y, Fan Y, Wu D, Pei Y (2008) An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker. Biotechnol Lett 30(8):1379-1383
13. Jin K, Zhang Y, Fang W, Luo Z, Zhou Y, Pei Y (2010) Carboxylate transporter gene JEN1 from the entomopathogenic fungus Beauveria bassiana is involved in conidiation and virulence. Appl Environ Microb 76(1):254-263
14. Lord JC, Hartzer K, Toutges M, Oppert B (2010) Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge. J Microbiol Meth 80(2):219-221
15. Radoni A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A (2004) Guideline to reference gene selection for quantitative real-time PCR. Biochem Bioph Res Co 313(4):856-862 (Pubitemid 38050071)
16. Teste MA, Duquenne M, Francois JM, Parrou JL (2009) Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae. BMC Mol Bio 10:99
17. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75:291-295 (Pubitemid 29486627)
18. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Bio 3(7):R0034
19. Vandesompele J, Kubista M, Pfaffl MW (2009) Reference gene validation software for improved normalization. In: Logan J (ed) Real-time PCR: current technology and applications, 1st edn. Caister Academic, London, pp 47-64
20. Wan CY, Wilkins TA (1994) A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Anal Biochem 223(1):7-12 (Pubitemid 24356684)
21. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39(1):75-85
22. Xiao Y, Luo M, Fang W, Luo K, Hou L, Luo X, Pei Y (2002) PCR walking in cotton genome using YADE method. Acta Genet Sinica 29(1):62-66 (Pubitemid 135683882)
23. Zhang Y, Zhao J, Fang W, Zhang J, Luo Z, Zhang M, Fan Y, Pei Y (2009) Mitogen-activated protein kinase hog1 in the entomopathogenic fungus Beauveria bassiana regulates environmental stress responses and virulence to insects. Appl Environ Microb 75(11):3787-3795
24. Zhang Y, Zhang J, Jiang X, Wang G, Luo Z, Fan Y, Wu Z, Pei Y (2010) Requirement of a mitogen-activated protein kinase for appressorium formation and penetration of insect cuticle by the entomopathogenic fungus Beauveria bassiana. Appl Environ Microb 76(7):2262-2270