Enhanced selection for homologous-recombinant embryonic stem cell clones by Cre recombinase-mediated deletion of the positive selection marker

Transgenic Research

Volumn 21, Issue 1, 2012, Pages 227-229

  Bouabe, Hicham ^a   Moser, Markus ^b   Heesemann, Jürgen ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; INTEGRASE;

ANIMAL; ARTICLE; EMBRYONIC STEM CELL; GENETIC MARKER; GENETIC SELECTION; GENETIC TRANSFECTION; GENETICS; HOMOLOGOUS RECOMBINATION; MOUSE; PROMOTER REGION; SIMPLEXVIRUS;

ANIMALS; EMBRYONIC STEM CELLS; GENETIC MARKERS; HOMOLOGOUS RECOMBINATION; INTEGRASES; MICE; PROMOTER REGIONS, GENETIC; SELECTION, GENETIC; SIMPLEXVIRUS; TRANSFECTION;

EID: 84856217353     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-011-9522-x     Document Type: Article

References (5)

1. Baldwin EP, Martin SS, Abel J, Gelato KA, Kim H, Schultz PG, Santoro SW (2003) A specificity switch in selected cre recombinase variants is mediated by macromolecular plasticity and water. Chem Biol 10: 1085-1094.
2. Buchholz F, Stewart AF (2001) Alteration of Cre recombinase site specificity by substrate-linked protein evolution. Nat Biotechnol 19: 1047-1052.
3. Dymecki SM (1996) Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93: 6191-6196.
4. Gu H, Zou YR, Rajewsky K (1993) Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 73: 1155-1164.
5. Lee KF, Li E, Huber LJ, Landis SC, Sharpe AH, Chao MV, Jaenisch R (1992) Targeted mutation of the gene encoding the low affinity NGF receptor p75 leads to deficits in the peripheral sensory nervous system. Cell 69: 737-749.