Improved RP-HPLC separation of Hg 2+ and CH 3Hg + using a mixture of thiol-based mobile phase additives

Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

Volumn 47, Issue 1, 2012, Pages 149-154

  Meers, Jeffrey D ^a   Jahromi, Elham Zeini ^a   Heyne, Belinda ^a   Gailer, Jürgen ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

HPLC; ICP AES; Mercuric mercury; methylmercury; separation

Indexed keywords

ANALYSIS TIME; ANTHROPOGENIC POLLUTION; AQUEOUS MOBILE PHASE; BASELINE SEPARATION; ENVIRONMENTAL WATER; FREE IONS; HPLC; HPLC SEPARATION; HUMIC ACID; ICP-AES; INDUCTIVELY COUPLED PLASMA MASS SPECTROMETER; L-CYSTEINE; MERCURY SPECIES; METHYL MERCURY; MOBILE PHASE; MOBILE PHASE ADDITIVES; MOBILE PHASIS; N-ACETYLCYSTEINE; PHOSPHATE BUFFERS; RETENTION BEHAVIOR; RP-HPLC; SEPARATION METHODS; VOID VOLUME;

AMINO ACIDS; ATOMIC EMISSION SPECTROSCOPY; COLUMN CHROMATOGRAPHY; INDUCTIVELY COUPLED PLASMA; MERCURY (METAL); MERCURY COMPOUNDS; POLLUTION DETECTION;

SEPARATION;

ACETYLCYSTEINE; CYSTEINE; MERCURIC MERCURY; MERCURY; METHYLMERCURY; THIOL; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC EMISSION SPECTROMETRY; MASS SPECTROMETRY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE;

ACETYLCYSTEINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; MERCURY; METHYLMERCURY COMPOUNDS; SPECTROPHOTOMETRY, ATOMIC; WATER POLLUTANTS, CHEMICAL;

EID: 84856080945     PISSN: 10934529     EISSN: 15324117     Source Type: Journal    
DOI: 10.1080/10934529.2012.630305     Document Type: Article

References (17)

1. Emsley, J. The Elements, 3rd Ed., Clarendon Press, Oxford, 1998; 289 pp.
2. Poissant, L.; Pilote, M.; Beauvais, C.; Constant, P.; Zhang, H.H. A year of continuous measurements of three atmospheric mercury species (GEM, RGM and Hgp) in southern Quebec, Canada. Atmos. Environ. 2005, 39, 1275-1287. (Pubitemid 40249387)
3. Morel, F.M.M.; Kraepiel, A.M.L.; Amyot, M. The chemical cycle and bioaccumulation of mercury. Annu. Rev. Ecol. Syst. 1998, 29, 543-566. (Pubitemid 29033042)
4. Gailer, J. Chronic toxicity of AsIII in mammals: The role of (GS)2AsSe-. Biochimie 2009, 91, 1268-1272.
5. Hanisch, C. Where is mercury deposition coming from? Environ. Sci. Technol. 1998, 176A-179A.
6. Clarkson, T.W. The three modern faces of mercury. Environ. Health Perspect. 2002, 110 (Suppl. 1), 11-23. (Pubitemid 34163090)
7. Clarkson, T.W., Magos, L.; Myers, G.J. The toxicology of mercury-Current exposures and clinical manifestations. New Engl. J. Med. 2003, 349, 1731-1737. (Pubitemid 37315011)
8. Harris, H.H.; Pickering, I.J.; George, G.N. The chemical form of mercury in fish. Science 2003, 301, 1203. (Pubitemid 37052201)
9. Schober, S.E.; Sinks, T.H.; Jones, R.L.; Bolger, P.M.; McDowell, M.; Osterloh, J.; Garrett, E.S.; Canady, R.A.; Dillon, C.F.; Sun, Y.; Joseph, C.B.; Mahaffey, K.R. Blood mercury levels in US children and women of childbearing age, 1999-2000. J. Am. Med. Assoc. 2003, 289, 1667-1674. (Pubitemid 37430272)
10. Ng, D.K.-K.; Chan, C.-H.; Soo, M.T.; Lee, R. S.-Y. Low-level mercury exposure in children and adolescents: Meta-analysis. Pediatr. Intern. 2007, 49, 80-87. (Pubitemid 46146690)
11. Schaefer, J.K.; Yagi, J.; Reinfelder, J.R.; Ellickson, K.M.; Tel-Or, S.; Barkay, T. Role of the bacterial organomercury lyase (MerB) in controlling accumulation in mercury-contaminated natural waters. Environ. Sci. Technol. 2004, 38, 4304-4311. (Pubitemid 39095224)
12. Percy, A.J.; Korbas, M.; George, G.N.; Gailer, J. Reversed-phase high-performance liquid chromatographic separation of inorganic mercury and methylmercury driven by their different coordination chemistry towards thiols. J. Chromatogr. A 2007, 1156, 331-339. (Pubitemid 46935723)
13. Heumann, K.G. Metal complexes of humic substances. In Handbook of Elemental Speciation II: Species in the Environment, Food, Medicine & Occupational Health, ed. R. Cornelis, Crews, H., Caruso, J., Heumann, K.G.; John Wiley & Sons, Ltd., New York; 2005.
14. Ravichandran, M. Interactions between mercury and dissolved organic matter -A review. Chemosphere 2004, 55, 319-331. (Pubitemid 38252935)
15. Lin, Y.; Larssen, T.; Vogt, R.D.; Feng, X. Identification of fractions of mercury in water, soil and sediment from a typical Hg mining area inWanshan, Guizhou province, China. Appl. Geochem. 2010, 25, 60-68.
16. Xia, K.; Skyllberg, U.L.; Bleam, W.F.; Bloom, P.R.; Nater, E.A.; Helmke, P.A. X-ray absorption spectroscopic evidence for the complexation of Hg(II) by reduced sulfur in soil humic substances. Environ. Sci. Technol. 1999, 33, 257-261.
17. Pei, K.L.; Sooriyaarachchi,M.; Sherell,D.A.;George,G.N.;Gailer, J. Probing the coordination behavior of Hg2+, CH3Hg+, and Cd2+ towards mixtures of two biological thiols by HPLC-ICP-AES. J. Inorg. Biochem. 2011, 105, 375-381.