Purification and activity testing of the full-length YycFGHI proteins of Staphylococcus aureus

PLoS ONE

Volumn 7, Issue 1, 2012, Pages

  Türck, Michael ^a   Bierbaum, Gabriele ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; LIPOSOME; MUTANT PROTEIN; PHOSPHOLIPID; PHOSPHOTRANSFERASE; PROTEIN VARIANT; SODIUM CHLORIDE; UNCLASSIFIED DRUG; YYCF PROTEIN; YYCG PROTEIN; YYCH PROTEIN; YYCI PROTEIN; YYCF PROTEIN, BACTERIA;

ARTICLE; CELL MEMBRANE FLUIDITY; CONTROLLED STUDY; DNA BASE COMPOSITION; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; ENZYME RECONSTITUTION; IN VITRO STUDY; INTRACELLULAR SIGNALING; MICELLE; MICROENVIRONMENT; MOLECULAR CLONING; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; OPERON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; STAPHYLOCOCCUS AUREUS; WILD TYPE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHOSPHORYLATION;

POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; LIPOSOMES; PHOSPHORYLATION; STAPHYLOCOCCUS AUREUS;

EID: 84856061136     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0030403     Document Type: Article

References (50)

1. Martin PK, Li T, Sun D, Biek DP, Schmid MB, (1999) Role in cell permeability of an essential two-component system in Staphylococcus aureus. J Bacteriol 181: 3666-3673.
2. Fabret C, Hoch JA, (1998) A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. J Bacteriol 180: 6375-6383.
3. Ng WL, Winkler ME, (2004) Singular structures and operon organizations of essential two-component systems in species of Streptococcus. Microbiology 150: 3096-3098.
4. Dubrac S, Bisicchia P, Devine K, Msadek T, (2008) A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway. Mol Microbiol 70: 1307-1322.
5. Szurmant H, Nelson K, Kim EJ, Perego M, Hoch JA, (2005) YycH regulates the activity of the essential YycFG two-component system in Bacillus subtilis. J Bacteriol 187: 5419-5426.
6. Szurmant H, Mohan MA, Imus PM, Hoch JA, (2007) YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis. J Bacteriol 189: 3280-3289.
7. Szurmant H, White RA, Hoch JA, (2007) Sensor complexes regulating two-component signal transduction. Curr Opin Struct Biol 17: 706-715.
8. Winkler ME, Hoch JA, (2008) Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria. J Bacteriol 190: 2645-2648.
9. Howell A, Dubrac S, Andersen KK, Noone D, Fert J, et al. (2003) Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach. Mol Microbiol 49: 1639-1655.
10. Bisicchia P, Noone D, Lioliou E, Howell A, Quigley S, et al. (2007) The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis. Mol Microbiol 65: 180-200.
11. Dubrac S, Msadek T, (2004) Identification of genes controlled by the essential YycG/YycF two-component system of Staphylococcus aureus. J Bacteriol 186: 1175-1181.
12. Dubrac S, Boneca IG, Poupel O, Msadek T, (2007) New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus. J Bacteriol 189: 8257-8269.
13. Delaune A, Poupel O, Mallet A, Coic YM, Msadek T, et al. (2011) Peptidoglycan crosslinking relaxation plays an important role in Staphylococcus aureus WalKR-dependent cell viability. PLoS One 6: e17054.
14. Jansen A, Türck M, Szekat C, Nagel M, Clever I, et al. (2007) Role of insertion elements and yycFG in the development of decreased susceptibility to vancomycin in Staphylococcus aureus. Int J Med Microbiol 297: 205-215.
15. Friedman L, Alder JD, Silverman JA, (2006) Genetic changes that correlate with reduced susceptibility to daptomycin in Staphylococcus aureus. Antimicrob Agents Chemother 50: 2137-2145.
16. Mwangi MM, Wu SW, Zhou Y, Sieradzki K, de Lencastre H, et al. (2007) Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. Proc Natl Acad Sci USA 104: 9451-9456.
17. Clausen VA, Bae W, Throup J, Burnham MK, Rosenberg M, et al. (2003) Biochemical characterization of the first essential two-component signal transduction system from Staphylococcus aureus and Streptococcus pneumoniae. J Mol Microbiol Biotechnol 5: 252-260.
18. Gutu AD, Wayne KJ, Sham LT, Winkler ME, (2010) Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain. J Bacteriol 192: 2346-2358.
19. Miroux B, Walker JE, (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260: 289-298.
20. Sass P, Bierbaum G, (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol 73: 347-352.
21. Santelli E, Liddington RC, Mohan MA, Hoch JA, Szurmant H, (2007) The crystal structure of Bacillus subtilis YycI reveals a common fold for two members of an unusual class of sensor histidine kinase regulatory proteins. J Bacteriol 189: 3290-3295.
22. Szurmant H, Zhao H, Mohan MA, Hoch JA, Varughese KI, (2006) The crystal structure of YycH involved in the regulation of the essential YycFG two-component system in Bacillus subtilis reveals a novel tertiary structure. Protein Sci 15: 929-934.
23. Fukushima T, Furihata I, Emmins R, Daniel RA, Hoch JA, et al. (2011) A role for the essential YycG sensor histidine kinase in sensing cell division. Mol Microbiol 79: 503-522.
24. Fukushima T, Szurmant H, Kim EJ, Perego M, Hoch JA, (2008) A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis. Mol Microbiol 69: 621-632.
25. Szurmant H, Bu L, Brooks CL III, Hoch JA, (2008) An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins. Proc Natl Acad Sci USA 105: 5891-5896.
26. Schägger H, Cramer WA, von Jagow G, (1994) Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal Biochem 217: 220-230.
27. Nicke A, Baumert HG, Rettinger J, Eichele A, Lambrecht G, et al. (1998) P2X1 and P2X3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels. EMBO J 17: 3016-3028.
28. Heuberger EH, Veenhoff LM, Duurkens RH, Friesen RH, Poolman B, (2002) Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation. J Mol Biol 317: 591-600.
29. Charlton ID, Doherty AP, (2000) Electrolyte-induced structural evolution of Triton X-100 micelles. Journal of Physical Chemistry B 104: 8327-8332.
30. Molina-Bolivar JA, Aguiar J, Ruiz CC, (2002) Growth and hydration of Triton X-100 micelles in monovalent alkali salts: A light scattering study. Journal of Physical Chemistry B 106: 870-877.
31. Kumbhakar M, Goel T, Mukherjee T, Pal H, (2004) Role of micellar size and hydration on solvation dynamics: A temperature dependent study in Triton-X-100 and Brij-35 micelles. Journal of Physical Chemistry B 108: 19246-19254.
32. Kumbhakar M, Goel T, Mukherjee T, Pal H, (2005) Nature of the water molecules in the palisade layer of a triton X-100 micelle in the presence of added salts: A solvation dynamics study. J Phys Chem B 109: 14168-14174.
33. Kumbhakar M, Goel T, Mukherjee T, Pal H, (2005) Effect of lithium chloride on the palisade layer of the Triton-X-100 micelle: two sites for lithium ions as revealed by solvation and rotational dynamics studies. J Phys Chem B 109: 18528-18534.
34. Streletzky K, Phillies GDJ, (1995) Temperature-dependence of Triton X-100 micelle size and hydration. Langmuir 11: 42-47.
35. Jung K, Tjaden B, Altendorf K, (1997) Purification, reconstitution, and characterization of KdpD, the turgor sensor of Escherichia coli. J Biol Chem 272: 10847-10852.
36. Janausch IG, Garcia-Moreno I, Unden G, (2002) Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro. J Biol Chem 277: 39809-39814.
37. Möker N, Krämer J, Unden G, Krämer R, Morbach S, (2007) In vitro analysis of the two-component system MtrB-MtrA from Corynebacterium glutamicum. J Bacteriol 189: 3645-3649.
38. Qin Z, Zhang J, Xu B, Chen L, Wu Y, et al. (2006) Structure-based discovery of inhibitors of the YycG histidine kinase: new chemical leads to combat Staphylococcus epidermidis infections. BMC Microbiol 6: 96.
39. Yamamoto K, Kitayama T, Minagawa S, Watanabe T, Sawada S, et al. (2001) Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci Biotechnol Biochem 65: 2306-2310.
40. Howell A, Dubrac S, Noone D, Varughese KI, Devine K, (2006) Interactions between the YycFG and PhoPR two-component systems in Bacillus subtilis: the PhoR kinase phosphorylates the non-cognate YycF response regulator upon phosphate limitation. Mol Microbiol 59: 1199-1215.
41. Gurtovenko AA, Vattulainen I, (2008) Effect of NaCl and KCl on phosphatidylcholine and phosphatidylethanolamine lipid membranes: insight from atomic-scale simulations for understanding salt-induced effects in the plasma membrane. J Phys Chem B 112: 1953-1962.
42. Zhao W, Rog T, Gurtovenko AA, Vattulainen I, Karttunen M, (2008) Role of phosphatidylglycerols in the stability of bacterial membranes. Biochimie 90: 930-938.
43. Jordan S, Hutchings MI, Mascher T, (2008) Cell envelope stress response in Gram-positive bacteria. FEMS Microbiol Rev 32: 107-146.
44. Matias VR, Beveridge TJ, (2007) Cryo-electron microscopy of cell division in Staphylococcus aureus reveals a mid-zone between nascent cross walls. Mol Microbiol 64: 195-206.
45. Cybulski LE, Martin M, Mansilla MC, Fernandez A, de Mendoza D, (2010) Membrane thickness cue for cold sensing in a bacterium. Curr Biol 20: 1539-1544.
46. Amrein KE, Takacs B, Stieger M, Molnos J, Flint NA, et al. (1995) Purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL. Proc Natl Acad Sci USA 92: 1048-1052.
47. Reipert A, Ehlert K, Kast T, Bierbaum G, (2003) Morphological and genetic differences in two isogenic Staphylococcus aureus strains with decreased susceptibilities to vancomycin. Antimicrob Agents Chemother 47: 568-576.
48. Bradford MM, (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
49. Rigaud JL, Levy D, (2003) Reconstitution of membrane proteins into liposomes. Methods Enzymol 372: 65-86.
50. Rouser G, Fleischer S, Yamamoto A, (1970) Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5: 494-496.