Evaluation of salt concentrations, chromogenic media and anatomical sampling sites for detection of methicillin-resistant Staphylococcus aureus in pigs

Veterinary Microbiology

Volumn 154, Issue 3-4, 2012, Pages 363-368

  Pletinckx, L J ^a,b   De Bleecker, Y ^a   Dewulf, J ^c   Rasschaert, G ^d   Goddeeris, B M ^b   De Man, I ^a  

^a Catholic University College South West Flanders KATHO   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

^d INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

Author keywords

Anatomical sampling sites; Chromogenic media; MRSA ST398; Pigs

Indexed keywords

CHROMOGENIC SUBSTRATE; SODIUM CHLORIDE;

ANATOMICAL SAMPLING SITE; ARTICLE; BACTERIUM DETECTION; INTERMETHOD COMPARISON; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; NOSE; PERINEUM; SAMPLING; SENSITIVITY AND SPECIFICITY; SWINE;

ANIMALS; BACTERIAL TYPING TECHNIQUES; CULTURE MEDIA; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; SENSITIVITY AND SPECIFICITY; SODIUM CHLORIDE; STAPHYLOCOCCAL INFECTIONS; SWINE;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; SUIDAE;

EID: 84855956535     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.07.027     Document Type: Article

References (26)

1. Broens E.M., Graat E.A.M., van der Wolf P.J., van de Giessen A.W., van Duijkeren E., Wagenaar J.A., van Nes A., Mevius D.J., de Jong M.C.M. MRSA CC398 in the pig production chain. Prev. Vet. Med. 2011, 98:182-189.
2. Bruins M.J., Juffer P., Wolfhagen M.J.H.M., Ruijs G.J.H.M. Salt tolerance of methicillin-resistant and methicillin-susceptible Staphylococcus aureus. J. Clin. Microbiol. 2007, 45:682-683.
3. Cherkaoui A., Renzi G., François P., Schrenzel J. Comparison of four chromogenic media for culturebased screening of methicillin-resistant Staphylococcus aureus. J. Med. Microbiol. 2007, 56:500-503.
4. de Neeling A.J., van den Broek M.J.M., Spalburg E.C., van Santen-Verhuevel M.G., Dam-Deisz W.D.C., Boshuizen H.C., van de Giessen A.W., van Duijkeren E., Huijsdens X.W. High prevanlence of methicillin resistant Staphylococcus aureus in pigs. Vet. Microbiol. 2007, 122:366-372.
5. Dewaele I., Messens W., De Man I., Delputte P., Herman L., Butaye P., Heyndrickx M., Rasschaert G. Sampling, prevalence and characterization of methicillin-resistant Staphylococcus aureus on two Belgian pig farms. Vet. Sci. Dev. 2011, 1:e1.
6. Diederen B.M.W., van Leest M., van Duijn I., Willemse P., van Keulen P.H.J., Kluytmans J.A.J.W. Performance of MRSA ID, a new chromogenic medium for detection of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2006, 44:586-588.
7. Enright M.C., Day N.P., Davies C.E., Peacock S.J., Spratt B.G. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphlococcus aureus. J. Clin. Microbiol. 2000, 38:1008-1015.
8. Graveland H., van Duijkeren E., van Nes A., Schoormans A., Broekhuizen-Stins M., Oosting-van Schothorst I., Heederik D., Wagenaar J.A. Evaluation of isolation procedures and chromogenic agar media for the detection of MRSA in nasal swabs from pigs and veal calves. Vet. Microbiol. 2009, 139:121-125.
9. Harmsen D., Claus H., Witte W., Rothgänger J., Claus H., Turnwald D., Vogel U. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 2003, 41:5442-5448.
10. Jones E.M., Bowker K.E., Cooke R., Marshall R.J., Reeves D.S., MacGowan A.P. Salt tolerance of EMRSA-16 and its effect on the sensitivity of screening cultures. J. Hosp. Infect. 1997, 35:59-62.
11. Lee J.H. Methicillin (oxcacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl. Environ. Microbiol. 2003, 69:6489-6494.
12. Lina G., Boutite F., Tristan A., Bes M., Etienne J., Vandenesch F. Bacterial competition for human nasal cavity colonization: role of Staphylococcal agr Alleles. Appl. Environ. Microbiol. 2003, 69:18-23.
13. Maes N., Magdalena J., Rottiers S., De Gheldre Y., Streulens M.J. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures. J. Clin. Microbiol. 2002, 40:1514-1517.
14. Milheiriço C., Oliveira D.C., de Lencastre H. Multiplex PCR strategy for subtyping the Staphylococcal Cassette Chromosome mec type IV in methicillin-resistant Staphylococcus aureus:'SCCmecIV multiplex'. J. Antimicrob. Chemother. 2007, 60:42-48.
15. Nahimana I., Francioli P., Blanc D.S. Evaluation of three chromogenic media (MRSA-ID, MRSA-select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 2006, 12:1168-1174.
16. Nemati M., Hermans K., Lipinska U., Denis O., Deplano A., Streulens M., Devriese L., Pasmans F., Haesebrouck F. Antimicrobial resistance of old and recent Staphylococcus aureus isolates from poultry: first detection of livestock-associated methicillin-resistant strain ST398. Antimicrob. Agents Chemother. 2008, 52:3817-3819.
17. Nonhoff C., Denis O., Brenner A., Buidin P., Legros N., Thiroux C., Dramaix M., Streulens M.J. Comparison of three chromogenic media and enrichment broth media for the detection of Methicillin-Resistant Staphylococcus aureus from mucocutaneous screening specimens. Eur. J. Clin. Microbiol. Infect. Dis. 2009, 28:363-369.
18. Oliveira D.C., de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2002, 4:2155-2161.
19. Strandèn A., Frei R., Widmer A.F. Molecular typing of methicillin-resistant Staphylococcus aureus: Can PCR replace pulsed-field gel electrophoresis?. J. Clin. Microbiol. 2003, 41:3181-3186.
20. van den Eede A., Martens A., Lipinska U., Streulens M., Deplano A., Denis O., Haesebrouck F., Gasthuys F., Hermans K. High occurence of methicillin-resistant Staphylococus aureus ST398 in equine nasal samples. Vet. Microbiol. 2009, 133:138-144.
21. van Duijkeren E., Wolfhagen M.J., Heck M.E., Wannet W.J. Transmission of a Panton-Valentine leucocidin-positive, methicillin-resistant Staphylococcus aureus strain between humans and a dog. J. Clin. Infect. 2005, 43:6209-6211.
22. Vanderhaeghen W., Cerpentier T., Adriaensen C., Vicca J., Hermans K., Butaye P. Methicillin-resistant Staphylococcus aureus (MRSA) ST398 associated with clinical and subclinical mastitis in Belgian cows. Vet. Microbiol. 2010, 144:166-171.
23. Verkade E., Verhulst C., van Cleef B., Kluytmans J. Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 2011, 30:109-112.
24. Voss A., Loeffen F., Bakker J., Klaassen C., Wulf M. Methicillin-resistant Staphylococcus aureus in pig farming. Emerg. Infect. Dis. 2005, 11:1965-1966.
25. Wagenaar, J.A., van de Giessen, A.W. Veegerelateerde MRSA: epidemiologie in dierlijke productieketens, transmisie naar de mens en karakterisatie van de kloon. RIVM-rapport 330224001, in press. http://www.rivm.nl/bibliotheek/rapporten/330224001.pdf.
26. Zhang K., McClure J., Elsayed S., Louie T., Conly J.M. Novel multiplex PCR assay for characterization and concomitant subtyping of Staphylococcal Cassette Chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2005, 43:5026-5033.