Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: 'SCCmec IV multiplex'

Journal of Antimicrobial Chemotherapy

Volumn 60, Issue 1, 2007, Pages 42-48

  Milheiriço, Catarina ^a   Oliveira, Duarte C ^a   de Lencastre, Hermínia ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

CA MRSA; EMRSA 15; MRSA; SCCmec type IV; SCCmec typing; Subtype IVh

Indexed keywords

BACTERIAL DNA;

ALLOTYPE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA EXTRACTION; GENE AMPLIFICATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; VALIDATION PROCESS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CHROMOSOMES, BACTERIAL; DNA PRIMERS; HUMANS; METHICILLIN; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 34447565048     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkm112     Document Type: Article

References (27)

1. Ito T, Katayama Y, Hiramatsu K. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 1999; 43: 1449-58.
2. Katayama Y, Ito T, Hiramatsu K. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: Role of IS431-mediated mecl deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob Agents Chemother 2001; 45 1955-63.
3. Katayama Y, Ito T, Hiramatsu K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 2000; 44: 1549-55.
4. Ito T, Katayama. Y, Asada K et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2001; 45: 1323-36.
5. Oliveira DC, Milheirico C, de Lencastre H. Redefining a structural variant of the staphylocccocal cassette chromosome mec. SCCmec type VI. Antimicrob Agents Chemother 2006; 50: 3457-9.
6. Ito T, Ma XX, Takeuchi F et al. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 2004; 48: 2637-51.
7. Ma XX, Ito T, Tiensasitorn C et al. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 2002; 46: 1147-52.
8. Ito T, Okuma K, Ma XX et al. Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: Genomic island SCC. Drug Resist Updat 2003; 6: 41-52.
9. Furuya EY, Lowy FD. Antimicrobial-resistant bacteria in the community setting. Nat Rev Microbiol 2006; 4: 36-45.
10. Vandenesch F, Naimi T, Enright MC et al. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: Worldwide emergence. Emerg Infect Dis 2003; 9: 978-84.
11. Robinson DA, Enright MC. Evolutionary models of the emergence of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2003; 47: 3926-34.
12. Kwon NH, Park KT, Moon JS et al. Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J. Antimicrob Chemother 2005; 56: 624-32.
13. Shore A, Rossney AS, Keane CT et al. Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland. Antimicrob Agents Chemother 2005; 49: 2070-83.
14. Johnson AP, Pearson A, Duckworth G. Surveillance and epidemiology of MRSA bacteraemia in the UK. J Antimicrob Chemother 2005; 56: 455-62.
15. Okuma K, Iwakawa K, Tumidge JD et al. Dissemination of new mathicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 2002; 40: 4289-94.
16. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2002; 46: 2155-61.
17. Zhang K, McClure JA, Elsayed S et al. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2005; 43: 5026-33.
18. Oliveira DC, Tomasz A, de Lencastre H. The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: Identification of two ancestral genetic backgrounds and the associated mec elements. Microb Drug Resist 2001; 7: 349-61.
19. Kondo Y, Ito T, Ma XX et al. Combination of multiplex PCRs for SCCmec type assignment: Rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 2007; 51: 264-74.
20. Oliveira DC, Milheirico C, Vinga S et al. Assessment of allelic variation in the ccrAB locus in methicillin-resistant Staphylococcus aureus clones. J Antimicrob Chemother 2006; 58: 23-30.
21. Baba T, Takeuchi F, Kuroda M et al. Genome and virulence determinants of high virulence community-acquired MRSA. Lancet 2002; 359: 1819-27.
22. Adcock PM, Pastor P, Medley F et al. Methicillin-resistant Staphylococcus aureus in two child care centers. J Infect Dis 1998; 178: 577-80.
23. Harmony Project. http://www.harmony-microbe.net/ (27 April 2006, date last accessed).
24. de Lencastre H, Severina EP, Roberts RB et al. Testing the efficacy of a molecular surveillance network: Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF) genotypes in six hospitals in the metropolitan New York City area. Microb Drug Resist 1996; 2: 343-51.
25. Sa-Leao R, Santos Sanches I, Dias D et al. Detection of an archaic clone of Staphylococcus aureus with low-level resistance to methicillin in a pediatric hospital in Portugal and in international samples: Relics of a formerly widely disseminated strain? J Clin Microbiol 1999; 37: 1913-20.
26. Faria NA, Oliveira DC, Westh H et al. Epidemiology of emerging methicillin-resistant Staphylococcus aureus (MRSA) in Denmark: A nationwide study in a country with low prevalence of MRSA infection. J Clin Microbiol 2005; 43: 1836-42.
27. Harmsen D, Claus H, Witte W et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003; 41: 5442-8.