Connexin-based channels in astrocytes: How to study their properties

Methods in Molecular Biology

Volumn 814, Issue , 2012, Pages 283-303

  Giaume, Christian ^a   Orellana, Juan A ^b   Abudara, Verónica ^a,c   Sáez, Juan C ^d  

^a l'alimentation et l'Environnement   (France)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^c UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^d Pontificia Universidad Católica de Chile   (Chile)

Author keywords

Double voltage clamp technique; Dye coupling; Dye uptake; Gap junctions; Hemichannels

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; FLUORESCENT DYE; GAP JUNCTION PROTEIN;

ANIMAL; ARTICLE; ASTROCYTE; CELL COMMUNICATION; CELL MEMBRANE POTENTIAL; ELECTROPHYSIOLOGY; FLUORESCENCE MICROSCOPY; HISTOLOGY; METABOLISM; MOUSE; PATCH CLAMP; PHYSIOLOGY;

ANIMALS; ASTROCYTES; CELL COMMUNICATION; CONNEXINS; ELECTROPHYSIOLOGY; FLUORESCENT DYES; HEPES; HISTOLOGICAL TECHNIQUES; MEMBRANE POTENTIALS; MICE; MICROSCOPY, FLUORESCENCE; PATCH-CLAMP TECHNIQUES;

EID: 84855955656     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-452-0_19     Document Type: Article

References (55)

1. Volterra, A., Bezzi, P. (2002) Release of transmitters from glial cells, in The tripartite synapse: glial in synaptic transmission (Volterra A, M., P. and Haydon, P.G., Ed.), pp p 164- 182, Oxford University Press, Oxford.
2. Araque, A., Parpura, V., Sanzgiri, R. P., and Haydon, P. G. (1999) Tripartite synapses: glia, the unacknowledged partner, Trends Neurosci 22, 208-215. (Pubitemid 29202082)
3. Roerig, B., and Feller, M. B. (2000) Neurotransmitters and gap junctions in developing neural circuits, Brain Res Brain Res Rev 32, 86-114. (Pubitemid 30177312)
4. Harris, A. L. (2007) Connexin channel permeability to cytoplasmic molecules, Prog Biophys Mol Biol 94, 120-143. (Pubitemid 46782979)
5. Spray, D. C., Ye, Z. C., and Ransom, B. R. (2006) Functional connexin "hemichannels": A critical appraisal, Glia 54, 758-773. (Pubitemid 44629638)
6. Scemes, E., Suadicani, S. O., Dahl, G., and Spray, D. C. (2007) Connexin and pannexin mediated cell-cell communication, Neuron Glia Biol 3, 199-208. (Pubitemid 351594228)
7. Baranova, A., Ivanov, D., Petrash, N., Pestova, A., Skoblov, M., Kelmanson, I., Shagin, D., Nazarenko, S., Geraymovych, E., Litvin, O., Tiunova, A., Born, T. L., Usman, N., Staroverov, D., Lukyanov, S., and Panchin, Y. (2004) The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins, Genomics 83, 706-716. (Pubitemid 38340811)
8. Panchin, Y., Kelmanson, I., Matz, M., Lukyanov, K., Usman, N., and Lukyanov, S. (2000) A ubiquitous family of putative gap junction molecules, Curr Biol 10, R473-474. (Pubitemid 30466927)
9. Bruzzone, R., Hormuzdi, S. G., Barbe, M. T., Herb, A., and Monyer, H. (2003) Pannexins, a family of gap junction proteins expressed in brain, Proc Natl Acad Sci USA 100, 13644-13649. (Pubitemid 37444793)
10. Vanden Abeele, F., Bidaux, G., Gordienko, D., Beck, B., Panchin, Y. V., Baranova, A. V., Ivanov, D. V., Skryma, R., and Prevarskaya, N. (2006) Functional implications of calcium permeability of the channel formed by pannexin 1, J Cell Biol 174, 535-546. (Pubitemid 44231865)
11. Lai, C. P., Bechberger, J. F., Thompson, R. J., MacVicar, B. A., Bruzzone, R., and Naus, C. C. (2007) Tumor-suppressive effects of pannexin 1 in C6 glioma cells, Cancer Res 67, 1545-1554. (Pubitemid 46383378)
12. Houades, V., Koulakoff, A., Ezan, P., Seif, I., and Giaume, C. (2008) Gap junction-mediated astrocytic networks in the mouse barrel cortex, J Neurosci 28, 5207-5217.
13. Rouach, N., Koulakoff, A., Abudara, V., Willecke, K., and Giaume, C. (2008) Astroglial metabolic networks sustain hippocampal synaptic transmission, Science 322, 1551-1555.
14. Giaume, C., Koulakoff, A., Roux, L., Holcman, D., and Rouach, N. (2010) Astroglial networks: A step further in neuroglial and gliovascular interactions, Nat Rev Neurosci 11, 87-99.
15. Kunze, A., Congreso, M. R., Hartmann, C., Wallraff-Beck, A., Huttmann, K., Bedner, P., Requardt, R., Seifert, G., Redecker, C., Willecke, K., Hofmann, A., Pfeifer, A., Theis, M., and Steinhauser, C. (2009) Connexin expression by radial glia-like cells is required for neurogenesis in the adult dentate gyrus, Proc Natl Acad Sci USA 106, 11336-11341.
16. Wallraff, A., Kohling, R., Heinemann, U., Theis, M., Willecke, K., and Steinhauser, C. (2006) The impact of astrocytic gap junctional coupling on potassium buffering in the hippocampus, J Neurosci 26, 5438-5447. (Pubitemid 44318396)
17. Orellana, J. A., Sáez, P. J., Shoji, K. F., Schalper, K. A., Palacios-Prado, N., Velarde, V., Giaume, C., Bennett, M. V., and Sáez, J. C. (2009) Modulation of brain hemichannels and gap junction channels by pro-infl ammatory agents and their possible role in neurodegeneration, Antioxid Redox Signal 11, 369-399.
18. Giaume, C., and Theis, M. 2009) Pharmacological and genetic approaches to study connexin-mediated channels in glial cells of the central nervous system Brain Res Rev
19. Kang, J., Kang, N., Lovatt, D., Torres, A., Zhao, Z., Lin, J., and Nedergaard, M. (2008) Connexin 43 hemichannels are permeable to ATP, J Neurosci 28, 4702-4711.
20. Neyton, J., and Trautmann, A. (1985) Singlechannel currents of an intercellular junction, Nature 317, 331-335.
21. Spray, D. C., Harris, A. L., and Bennett, M. V. (1979) Voltage dependence of junctional conductance in early amphibian embryos, Science 204, 432-434. (Pubitemid 9157312)
22. Giaume, C., Fromaget, C., el Aoumari, A., Cordier, J., Glowinski, J., and Gros, D. (1991) Gap junctions in cultured astrocytes: singlechannel currents and characterization of channel- forming protein, Neuron 6, 133-143.
23. Dermietzel, R., Hertberg, E. L., Kessler, J. A., and Spray, D. C. (1991) Gap junctions between cultured astrocytes: immunocytochemical, molecular, and electrophysiological analysis, J Neurosci 11, 1421-1432.
24. Même, W., Ezan, P., Venance, L., Glowinski, J., and Giaume, C. (2004) ATP-induced inhibition of gap junctional communication is enhanced by interleukin-1 beta treatment in cultured astrocytes, Neuroscience 126, 95-104. (Pubitemid 38609109)
25. Rouach, N., Pebay, A., Meme, W., Cordier, J., Ezan, P., Etienne, E., Giaume, C., and Tence, M. (2006) S1P inhibits gap junctions in astrocytes: involvement of G and Rho GTPase/ROCK, Eur J Neurosci 23, 1453-1464.
26. Venance, L., Piomelli, D., Glowinski, J., and Giaume, C. (1995) Inhibition by anandamide of gap junctions and intercellular calcium signalling in striatal astrocytes, Nature 376, 590-594.
27. Meme, W., Vandecasteele, M., Giaume, C., and Venance, L. (2009) Electrical coupling between hippocampal astrocytes in rat brain slices, Neurosci Res 63, 236-243.
28. Xu, G., Wang, W., Kimelberg, H. K., and Zhou, M. Electrical coupling of astrocytes in rat hippocampal slices under physiological and simulated ischemic conditions, Glia 58, 481-493.
29. Blomstrand, F., Venance, L., Siren, A. L., Ezan, P., Hanse, E., Glowinski, J., Ehrenreich, H., and Giaume, C. (2004) Endothelins regulate astrocyte gap junctions in rat hippocampal slices, Eur J Neurosci 19, 1005-1015. (Pubitemid 38297038)
30. Maglione, M., Tress, O., Haas, B., Karram, K., Trotter, J., Willecke, K., and Kettenmann, H. Oligodendrocytes in mouse corpus callosum are coupled via gap junction channels formed by connexin47 and connexin32, Glia 58, 1104-1117.
31. Venance, L., Cordier, J., Monge, M., Zalc, B., Glowinski, J., and Giaume, C. (1995) Homotypic and heterotypic coupling mediated by gap junctions during glial cell differentiation in vitro, Eur J Neurosci 7, 451-461.
32. Chvatal, A., Pastor, A., Mauch, M., Sykova, E., and Kettenmann, H. (1995) Distinct populations of identifi ed glial cells in the developing rat spinal cord slice: ion channel properties and cell morphology, Eur J Neurosci 7, 129-142.
33. Zhou, M., Schools, G. P., and Kimelberg, H. K. (2006) Development of GLAST(+) astrocytes and NG2(+) glia in rat hippocampus CA1: mature astrocytes are electrophysiologically passive, J Neurophysiol 95, 134-143. (Pubitemid 44698923)
34. Bennett, M. V. (1977) Electrical transmission: A functional analysis and comparison with chemical transmission, in Cellular Biology of Neurons, vol. I, sec. I, Handbook of Physiology. The Nervous System (Kandel, E. R., Ed.), Williams and Wilkins, Baltimore.
35. Venance, L., Glowinski, J., and Giaume, C. (2004) Electrical and chemical transmission between striatal GABAergic output neurones in rat brain slices, J Physiol 559, 215-230. (Pubitemid 39206964)
36. Neher, E., and Sakmann, B. (1976) Singlechannel currents recorded from membrane of denervated frog muscle fi bres, Nature 260, 799-802.
37. Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981) Improved patchclamp techniques for high-resolution current recording from cells and cell-free membrane patches, Pfl ugers Arch 391, 85-100. (Pubitemid 11012976)
38. Contreras, J. E., Sáez, J. C., Bukauskas, F. F., and Bennett, M. V. (2003) Gating and regulation of connexin 43 (Cx43) hemichannels, Proc Natl Acad Sci USA 100, 11388-11393. (Pubitemid 37205945)
39. Retamal, M. A., Froger, N., Palacios-Prado, N., Ezan, P., Sáez, P. J., Sáez, J. C., and Giaume, C. (2007) Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinfl ammatory cytokines released from activated microglia, J Neurosci 27, 13781-13792. (Pubitemid 350278255)
40. Schalper, K. A., Palacios-Prado, N., Retamal, M.A., Shoji, K.F., Martínez, A.D., Sáez, J.C. (2008) Connexin hemichannels composition determines the FGF-1-induced membrane permeability and free [Ca 2+ ] i responses, Mol Biol Cell, 19, 3501-3513.
41. Sánchez, H. A., Orellana, J. A., Verselis, V. K., and Sáez, J. C. (2009) Metabolic inhibition increases activity of connexin-32 hemichannels permeable to Ca2+ in transfected HeLa cells, Am J Physiol Cell Physiol 297, C665-678.
42. Neher, E. (1992) Correction for liquid junction potentials in patch clamp experiments, Methods Enzymol 207, 123-131.
43. Bao, X., Lee, S. C., Reuss, L., and Altenberg, G. A. (2007) Change in permeant size selectivity by phosphorylation of connexin 43 gapjunctional hemichannels by PKC, Proc Natl Acad Sci USA 104, 4919-4924. (Pubitemid 47186152)
44. Retamal, M. A., Cortés, C. J., Reuss, L., Bennett, M. V., and Sáez, J. C. (2006) S-nitrosylation and permeation through connexin 43 hemichannels in astrocytes: induction by oxidant stress and reversal by reducing agents, Proc Natl Acad Sci USA 103, 4475-4480.
45. D'Ambrosio, R., Wenzel, J., Schwartzkroin, P. A., McKhann, G. M., 2nd, and Janigro, D. (1998) Functional specialization and topographic segregation of hippocampal astrocytes, J Neurosci 18, 4425-4438. (Pubitemid 28280154)
46. Giaume, C. (1991) Application of the patch-clamp technique to the study of junctional conductance, in Biophysiscs of gap junctions (C, P., Ed.), CRC press.
47. Cotrina, M. L., Gao, Q., Lin, J. H., and Nedergaard, M. (2001) Expression and function of astrocytic gap junctions in aging, Brain Res 901, 55-61. (Pubitemid 32718805)
48. Soroceanu, L., Manning, T. J., Jr., and Sontheimer, H. (2001) Reduced expression of connexin-43 and functional gap junction coupling in human gliomas, Glia 33, 107-117. (Pubitemid 32160974)
49. Goldberg, G. S., Bechberger, J. F., and Naus, C. C. (1995) A pre-loading method of evaluating gap junctional communication by fl uorescent dye transfer, Biotechniques 18, 490-497.
50. Koulakoff, A., Ezan, P., and Giaume, C. (2008) Neurons control the expression of connexin 30 and connexin 43 in mouse cortical astrocytes, Glia 56, 1299-1311.
51. Siller-Jackson, A. J., Burra, S., Gu, S., Xia, X., Bonewald, L. F., Sprague, E., and Jiang, J. X. (2008) Adaptation of connexin 43-hemichannel prostaglandin release to mechanical loading, J Biol Chem 283, 26374-26382.
52. Muller, T., Moller, T., Neuhaus, J., and Kettenmann, H. (1996) Electrical coupling among Bergmann glial cells and its modulation by glutamate receptor activation, Glia 17, 274-284. (Pubitemid 26309420)
53. Houades V, Rouach, N., Ezan, P., Kirchhoff, F., Koulakoff, A., Giaume, C. (2006) Shapes of astrocyte networks in the juvenile brain, in Neuron Glia Biology, pp 3-14. (Pubitemid 43248059)
54. Nolte, C., Matyash, M., Pivneva, T., Schipke, C. G., Ohlemeyer, C., Hanisch, U. K., Kirchhoff, F., and Kettenmann, H. (2001) GFAP promoter-controlled EGFP-expressing transgenic mice: A tool to visualize astrocytes and astrogliosis in living brain tissue, Glia 33, 72-86. (Pubitemid 32102818)
55. Barres, B. A. (2008) The mystery and magic of glia: A perspective on their roles in health and disease, Neuron 60, 430-440.