The isolation of high quality RNA from the fruit of avocado (Persea americana Mill.)

South African Journal of Botany

Volumn 78, Issue , 2012, Pages 44-46

  Djami Tchatchou, A T ^a   Straker, C J ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

Avocado fruit; CDNA; CTAB; Flesh; Polyphenols; Polysaccharides; Proteins; RNA extraction; Skin

Indexed keywords

BROMIDE; ELECTROKINESIS; FRUIT; NUCLEIC ACID; PHENOL; POLYSACCHARIDE; PROTEIN; RNA; SKIN; SPECTROPHOTOMETRY;

PERSEA AMERICANA;

EID: 84855929088     PISSN: 02546299     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.sajb.2011.04.009     Document Type: Article

References (12)

1. Bergh B. Nutritious Value of Avocado. California Avocado Society Yearbook 1992, 76:123-135. California.
2. Chang S., Puryear J., Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter 1993, 11:113-116.
3. Fang G., Hammar S., Grumet R. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. BioTechniques 1992, 13:52-55.
4. Gasic K., Hernandez A., Korban S.S. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Molecular Biology Reporter 2004, 22:437a-437g.
5. Hu G.C., Honda C.H., Kita M.Y., Zhang Z., Tsuda T., Moriguchi T. A simple protocol RNA isolation from fruit trees containing high levels of polysaccharides and polyphenol compounds. Plant Molecular Biology Reporter 2002, 20:69a-69g.
6. Logemann J., Schell J., Willmitzer L. Improved method for the isolation of RNA from plant tissues. Analytical Biochemistry 1987, 163:16-20.
7. López-Gómez V.M. Some biochemical properties of polyphenol oxidase from two varieties of avocado. Food Chemistry 2002, 77:163-169.
8. López-Gómez R., Gómez-Lim M.A. A method for extracting intact RNA from fruits rich in polysaccharides using ripe mango mesocarp. HortScience 1992, 27:440-442.
9. Manning K. Isolation of nucleic acids from plants by differential solvent precipitation. Analytical Biochemistry 1991, 195:45-50.
10. Naveh E., Werman M.J., Sabo E., Neeman I. Defatted avocado pulp reduces body weight and total hepatic fat but increases plasma cholesterol in male rats fed diets with cholesterol. The Journal of Nutrition 2002, 132:2015-2018.
11. Pandit S.S., Mitra S.S., Giri A.P., Gupta V.S. A quick method for isolating RNA from raw and ripe fleshy fruits as well as for co-isolating DNA and RNA from polysaccharide and polyphenoI-rich leaf tissues. Journal of Plant Biology 2007, 50:60-64.
12. Valderrama-Cháirez M.L., Paredes-López O., Cruz-Hernández A. Isolation of functional RNA from cactus fruit. Plant Molecular Biology Reporter 2002, 20:279-286.