Imaging in vivo astrocyte/endothelial cell interactions at the blood-brain barrier

Methods in Molecular Biology

Volumn 814, Issue , 2012, Pages 515-529

  Willis, Colin L ^a  

^a UNIVERSITY OF NEW ENGLAND   (United States)

Author keywords

Astrocytes; Claudin; Confocal microscopy; GFAP; Glia immunofluorescence; PECAM 1; SDS PAGE; Vascular endothelium; Western blot

Indexed keywords

ALPHA CHLOROHYDRIN;

ANIMAL; ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; CELL COMMUNICATION; CELL HYPOXIA; CONFOCAL MICROSCOPY; CYTOLOGY; ENDOTHELIUM CELL; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; TIGHT JUNCTION; WESTERN BLOTTING;

ALPHA-CHLOROHYDRIN; ANIMALS; ASTROCYTES; BLOOD-BRAIN BARRIER; BLOTTING, WESTERN; CELL COMMUNICATION; CELL HYPOXIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTHELIAL CELLS; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; RATS; TIGHT JUNCTIONS;

EID: 84855917436     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-452-0_34     Document Type: Article

References (14)

1. Wolburg, H, and Lippoldt, A. (2002) Tight junctions of the blood-brain barrier: development, composition and regulation. Vasc. Pharmacol. 38, 323-337 (Pubitemid 36398254)
2. Abbott, N.J. (2002) Astrocyte-endothelial interactions and blood-brain barrier permeability. J. Anat. 200, 629-638. (Pubitemid 34823054)
3. Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, S., and Tsukita, S. (1993) Occludin-a novel integral membraneprotein localizing at tight junctions. J. Cell Biol. 123, 1777-1788. (Pubitemid 24012933)
4. Kniesel, U., and Wolburg, H. (2000) Tight junctions of the blood-brain barrier. Cell Mol. Neurobiol. 20, 57-76. (Pubitemid 30090916)
5. Plumb, J., McQuaid, S., Mirakhur, M., and Kirk, J. (2002) Abnormal endothelial tight junctions in active lesions and normal-appearing white matter in multiple sclerosis. Brain Pathol. 12, 154-169. (Pubitemid 34259917)
6. Abbott, N.J., Ronnback, L., Hansson, E. (2006) Astrocyte-endothelial interactions at the blood-brain barrier. Nature Rev. 7, 41-53. (Pubitemid 41828930)
7. Rubin, L.L., Barbu, K., Bard, F., Cannon, C., Hall, D.E., Horner, H., Janatpour, M., Liaw, C., Manning, K., Morales, J., Porter, S., Tanner, L., Tomaselli, K., and Yednock, T. (1991) Differentiation of brain endothelial-cells in cell culture. Ann. NY Acad. Sci. 633, 420-425.
8. Sobue, K., Yamamoto, N., Yoneda, K., Hodgson, M.E., Yamashiro, K., Tsuruoka, N., Tsuda, T., Katsuya, H., Miura, Y., Asai, K., and Kato, T. (1999) Induction of blood-brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors. Neurosci. Res. 35, 155- 164. (Pubitemid 29518406)
9. Prat, A., Biernacki, K., Wosik, K., Antel, J.P. (2001) Glial cell infl uence on the human bloodbrain barrier. Glia 36, 145-155. (Pubitemid 33055903)
10. Willis, C.L., Leach, L., Clarke, G.J., Nolan, C.C., and Ray, D.E. (2004) Reversible disruption of tight junction complexes in the rat blood-brain barrier, following transitory focal astrocyte loss. Glia 48, 1-13. (Pubitemid 39390843)
11. Willis, C.L., Nolan, C.C., Reith, S.N., Lister, T., Guerin, C.J., Mavroudis, G., Prior M.J.W., and Ray, D.E. (2004) Focal astrocyte loss is followed by microvascular damage, with subsequent repair of the blood-brain barrier in the apparent absence of direct astrocytic contact. Glia 45, 325-337. (Pubitemid 38332433)
12. Willis, C.L., Meske, D.S., and Davis, T.P. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. JCBFM 30 :1847-1859.
13. Witt, K.A., Mark, K.S., Hom, S., Davis, T.P. (2003) Effects of hypoxia-reoxygenation on rat blood-brain barrier permeability and junctional protein expression. Am. J. Physiol. Heart Circ. Physiol. 285 :H2820-2831. (Pubitemid 37443103)
14. Rigor, R.R., Hawkins, B.T., Miller, D.S. (2010) Activation of PKC isoform I at the blood- brain barrier rapidly decreases P-glycoprotein activity and enhances drug delivery to the brain. JCBFM 30 :1373-1383.