Using non-homologous end-joining-deficient strains for functional gene analyses in filamentous fungi

Methods in Molecular Biology

Volumn 835, Issue , 2012, Pages 133-150

  Arentshorst, Mark ^a   Ram, Arthur F J ^a,b   Meyer, Vera ^a,c  

^a LEIDEN UNIVERSITY   (Netherlands)

^b KLUYVER CENTRE FOR GENOMICS OF INDUSTRIAL FERMENTATION   (Netherlands)

^c TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

AMA1; Aspergillus; Complementation; Heterokaryon rescue; Ku70; NHEJ; Protoplast mediated transformation

Indexed keywords

ARTICLE; ASPERGILLUS; DNA END JOINING REPAIR; FUNGAL GENE; GENE DELETION; GENE SILENCING; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PROTOPLAST;

ASPERGILLUS; DNA END-JOINING REPAIR; GENE DELETION; GENE SILENCING; GENES, FUNGAL; GENETIC VECTORS; PROTOPLASTS; TRANSFORMATION, GENETIC;

ASPERGILLUS; FUNGI;

EID: 84855887130     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-501-5_9     Document Type: Article

References (21)

1. Ninomiya, Y., Suzuki, K., Ishii, C., and Inoue, H. (2004) Highly effi cient gene replacements in Neurospora strains defi cient for nonhomologous end-joining, Proc Natl Acad Sci USA 101, 12248-12253. (Pubitemid 39145429)
2. Kuck, U., and Hoff, B. (2010) New tools for the genetic manipulation of fi lamentous fungi, Appl Microbiol Biotechnol 86, 51-62.
3. Meyer, V. (2008) Genetic engineering of fi lamentous fungi - progress, obstacles and future trends, Biotechnol Adv 26, 177-185.
4. Dudasova, Z., Dudas, A., and Chovanec, M. (2004) Non-homologous end-joining factors of Saccharomyces cerevisiae, FEMS Microbiol Rev 28, 581-601. (Pubitemid 39469921)
5. Krogh, B. O., and Symington, L. S. (2004) Recombination proteins in yeast, Annu Rev Genet 38, 233-271. (Pubitemid 40013729)
6. Shrivastav, M., De Haro, L. P., and Nickoloff, J. A. (2008) Regulation of DNA double-strand break repair pathway choice, Cell Res 18, 134-147.
7. Nayak, T., Szewczyk, E., Oakley, C. E., Osmani, A., Ukil, L., Murray, S. L., Hynes, M. J., Osmani, S. A., and Oakley, B. R. (2006) A versatile and effi cient gene-targeting system for Aspergillus nidulans, Genetics 172, 1557-1566.
8. Osmani, A. H., Oakley, B. R., and Osmani, S. A. (2006) Identifi cation and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique, Nat Protoc 1, 2517-2526.
9. Kito, H., Fujikawa, T., Moriwaki, A., Tomono, A., Izawa, M., Kamakura, T., Ohashi, M., Sato, H., Abe, K., and Nishimura, M. (2008) MgLig4, a homolog of Neurospora crassa Mus-53 (DNA ligase IV), is involved in, but not essential for, non-homologous end-joining events in Magnaporthe grisea, Fungal Genet Biol 45, 1543-1551.
10. Malik, M., Nitiss, K. C., Enriquez-Rios, V., and Nitiss, J. L. (2006) Roles of nonhomologous end-joining pathways in surviving topoisomerase II-mediated DNA damage, Mol Cancer Ther 5, 1405-1414. (Pubitemid 44070476)
11. Meyer, V., Arentshorst, M., El-Ghezal, A., Drews, A. C., Kooistra, R., van den Hondel, C. A., and Ram, A. F. (2007) Highly effi cient gene targeting in the Aspergillus niger kusA mutant, J Biotechnol 128, 770-775. (Pubitemid 46341493)
12. Snoek, I. S., van der Krogt, Z. A., Touw, H., Kerkman, R., Pronk, J. T., Bovenberg, R. A., van den Berg, M. A., and Daran, J. M. (2009) Construction of an hdfA Penicillium chrysogenum strain impaired in non-homologous endjoining and analysis of its potential for functional analysis studies, Fungal Genet Biol 46, 418-426.
13. Nielsen, J. B., Nielsen, M. L., and Mortensen, U. H. (2008) Transient disruption of nonhomologous end-joining facilitates targeted genome manipulations in the fi lamentous fungus Aspergillus nidulans, Fungal Genet Biol 45, 165-170. (Pubitemid 351192333)
14. Carvalho, N. D., Arentshorst, M., Jin Kwon, M., Meyer, V., and Ram, A. F. (2010) Expanding the ku70 toolbox for fi lamentous fungi: establishment of complementation vectors and recipient strains for advanced gene analyses, Appl Microbiol Biotechnol 87, 1463-1473.
15. Michielse, C. B., Hooykaas, P. J., van den Hondel, C. A., and Ram, A. F. (2008) Agrobacterium -mediated transformation of the fi lamentous fungus Aspergillus awamori, Nat Protoc 3, 1671-1678.
16. Meyer, V., Mueller, D., Strowig, T., and Stahl, U. (2003) Comparison of different transformation methods for Aspergillus giganteus, Curr Genet 43, 371-377. (Pubitemid 37021064)
17. Michielse, C. B., Hooykaas, P. J., van den Hondel, C. A., and Ram, A. F. (2005) Agrobacterium -mediated transformation as a tool for functional genomics in fungi, Curr Genet 48, 1-17. (Pubitemid 41086352)
18. de Ruiter-Jacobs, Y. M., Broekhuijsen, M., Unkles, S. E., Campbell, E. I., Kinghorn, J. R., Contreras, R., Pouwels, P. H., and van den Hondel, C. A. (1989) A gene transfer system based on the homologous pyrG gene and effi - cient expression of bacterial genes in Aspergillus oryzae, Curr Genet 16, 159-163.
19. Tilburn, J., Scazzocchio, C., Taylor, G. G., Zabicky-Zissman, J. H., Lockington, R. A., and Davies, R. W. (1983) Transformation by integration in Aspergillus nidulans, Gene 26, 205-221. (Pubitemid 14190474)
20. Bennett, J. W., and Lasure, L. (1991) More gene manipulations in fungi, Academic Press, San Diego, pp 441-47.
21. van Hartingsveldt, W., Mattern, I. E., van Zeijl, C. M., Pouwels, P. H., and van den Hondel, C. A. (1987) Development of a homologous transformation system for Aspergillus niger based on the pyrG gene, Mol Gen Genet 206, 71-75. (Pubitemid 17014167)