The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer

Biosensors and Bioelectronics

Volumn 32, Issue 1, 2012, Pages 148-154

  Zou, Mingjian ^a   Chen, Yang ^a   Xu, Xiao ^a   Huang, Hongduan ^a   Liu, Feng ^a   Li, Na ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

6 Carboxyfluorescein (FAM); DNA aptamer; Dynamic fluorescence anisotropy; Fluorescence anisotropy; Lifetime; Lysozyme

Indexed keywords

6-CARBOXYFLUORESCEIN (FAM); ANALYTES; ANTIBODY-ANTIGEN; APTAMERS; BIOLOGICAL SAMPLES; DETECTING SYSTEMS; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ANISOTROPY; FLUORESCENCE ANISOTROPY METHODS; GLOBAL ROTATION; HIGH ANISOTROPY; HIGH SENSITIVITY; LIFETIME; LIMIT OF DETECTION; LINEAR DYNAMIC RANGES; LYSOZYME; MOLECULAR VOLUME; ROTATIONAL DYNAMICS; ROTATIONAL MOVEMENT; STANDARD ADDITION; TARGET MOLECULE; TIME RESOLVED MEASUREMENT;

ANISOTROPY; DNA; ENZYMES; FLUORESCENCE;

FLUOROPHORES;

6 CARBOXYFLUORESCEIN; AMYLASE; APTAMER; BOVINE SERUM ALBUMIN; CYTOCHROME C; HUMAN SERUM ALBUMIN; IMMUNOGLOBULIN; LYSOZYME; MAGNESIUM CHLORIDE; TRIACYLGLYCEROL LIPASE;

ANALYTIC METHOD; ANISOTROPY; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION CONSTANT; EMISSION ANISOTROPY; ENZYME ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; HUMAN; LIMIT OF DETECTION; PH; PROTEIN DNA BINDING; QUANTUM YIELD; ROTATION; SALIVA ANALYSIS; SENSITIVITY ANALYSIS; STEADY STATE; TEMPERATURE SENSITIVITY;

APTAMERS, NUCLEOTIDE; BASE SEQUENCE; FLUORESCEINS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HUMANS; LIMIT OF DETECTION; MURAMIDASE; RECOMBINANT PROTEINS; SALIVA;

EID: 84855759454     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2011.11.052     Document Type: Article

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