Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

Cellular and Molecular Biology Letters

Volumn 16, Issue 4, 2011, Pages 638-651

  Khan, Ammad Aslam ^a   Shimokawa, Takashi ^a   Strömblad, Staffan ^a   Zhang, Hongquan ^a,b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

Cell migration; Cis acting elements; CpG island; FERMT2; Gene expression; Gene regulation; Integrin; Kindlin 2; Mig 2; PLEKHC1; Promoter; Transcription factor SP1; Transcription start site

Indexed keywords

TRANSCRIPTION FACTOR SP1; MEMBRANE PROTEIN; MIG2B PROTEIN, HUMAN; RECOMBINANT PROTEIN; TUMOR PROTEIN;

ANIMAL CELL; ARTICLE; BASE PAIRING; BINDING SITE; CELL MIGRATION; COMPUTER MODEL; CONTROLLED STUDY; CPG ISLAND; DNA FLANKING REGION; FEMALE; GENE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE IDENTIFICATION; GENE LOCATION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; KINDLIN 2 GENE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN FUNCTION; TRANSCRIPTION REGULATION; ANIMAL; CELL STRAIN 3T3; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; GENETIC DATABASE; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HELA CELL; METABOLISM; MOLECULAR GENETICS; MOUSE; PHYSIOLOGY; PLASMID; PROTEIN TERTIARY STRUCTURE; REPORTER GENE; TRANSCRIPTION INITIATION SITE;

ANIMALS; BASE SEQUENCE; BINDING SITES; CERCOPITHECUS AETHIOPS; COS CELLS; DATABASES, GENETIC; GENE EXPRESSION REGULATION; GENES, REPORTER; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MICE; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; NIH 3T3 CELLS; PLASMIDS; PROMOTER REGIONS, GENETIC; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SP1 TRANSCRIPTION FACTOR; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 84855727428     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-011-0028-6     Document Type: Article

References (21)

1. Khan, A. A., Janke, A., Shimokawa, T. and Zhang, H. Phylogenetic analysis of kindlins suggests subfunctionalization of an ancestral unduplicated kindlin into three paralogs in vertebrates. Evol. Bioinform. Online7 (2011) 7-19.
2. Siegel, D. H., Ashton, G. H., Penagos, H. G., Lee, J. V., Feiler, H. S., Wilhelmsen, K. C., South, A. P., Smith, F. J., Prescott, A. R., Wessagowit, V., Oyama, N., Akiyama, M., Al Aboud, D., Al Aboud, K., Al Githami, A., Al Hawsawi, K., Al Ismaily, A., Al-Suwaid, R., Atherton, D. J., Caputo, R., Fine, J. D., Frieden, I. J., Fuchs, E., Haber, R. M., Harada, T., Kitajima, Y., Mallory, S. B., Ogawa, H., Sahin, S., Shimizu, H., Suga, Y., Tadini, G., Tsuchiya, K., Wiebe, C. B., Wojnarowska, F., Zaghloul, A. B., Hamada, T., Mallipeddi, R., Eady, R. A., McLean, W. H., McGrath, J. A. and Epstein, E. H. Loss of kindlin-1, a human homolog of the Caenorhabditis elegans actinextracellular-matrix linker protein UNC-112, causes Kindler syndrome. Am. J. Hum. Genet. 73 (2003) 174-187.
3. Rogalski, T. M., Mullen, G. P., Gilbert, M. M., Williams, B. D. and Moerman, D. G. The UNC-112 gene in Caenorhabditis elegans encodes a novel component of cell-matrix adhesion structures required for integrin localization in the muscle cell membrane. J. Cell Biol. 150 (2000) 253-264.
4. Tu, Y., Wu, W., Wu, T., Cao, Z., Wilkins, R., Toh, B. H., Cooper, M. E. and Chai, Z. Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways. J. Biol. Chem. 282 (2007) 11722-11731.
5. Mackinnon, A. C., Qadota, H., Norman, K. R., Moerman, D. G. and Williams, B. D. C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes. Curr. Biol. 12 (2002) 787-797.
6. Shi, X., Ma, Y. Q., Tu, Y., Chen, K., Wu, S., Fukuda, K., Qin, J., Plow, E. F. and Wu, C. The MIG-2/integrin interaction strengthens cell-matrix adhesion and modulates cell motility. J. Biol. Chem. 282 (2007) 20455-20466.
7. Dowling, J. J., Gibbs, E., Russell, M., Goldman, D., Minarcik, J., Golden, J. A. and Feldman, E. L. Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function. Circ. Res. 102 (2008) 423-431.
8. An, Z., Dobra, K., Lock, J. G., Stromblad, S., Hjerpe, A. and Zhang, H. Kindlin-2 is expressed in malignant mesothelioma and is required for tumor cell adhesion and migration. Int. J. Cancer127 (2010) 1999-2008.
9. Ussar, S., Wang, H. V., Linder, S., Fassler, R. and Moser, M. The Kindlins: subcellular localization and expression during murine development. Exp. Cell Res. 312 (2006) 3142-3151.
10. Montanez, E., Ussar, S., Schifferer, M., Bosl, M., Zent, R., Moser, M. and Fassler, R. Kindlin-2 controls bidirectional signaling of integrins. Genes Dev. 22 (2008) 1325-1330.
11. Kruger, M., Moser, M., Ussar, S., Thievessen, I., Luber, C. A., Forner, F., Schmidt, S., Zanivan, S., Fassler, R. and Mann, M. SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell134 (2008) 353-364.
12. Moser, M., Nieswandt, B., Ussar, S., Pozgajova, M. and Fassler, R. Kindlin-3 is essential for integrin activation and platelet aggregation. Nat. Med. 14 (2008) 325-330.
13. Malinin, N. L., Zhang, L., Choi, J., Ciocea, A., Razorenova, O., Ma, Y. Q., Podrez, E. A., Tosi, M., Lennon, D. P., Caplan, A. I., Shurin, S. B., Plow, E. F. and Byzova, T. V. A point mutation in KINDLIN3 ablates activation of three integrin subfamilies in humans. Nat. Med. 15 (2009) 313-318.
14. Suzuki, Y., Yamashita, R., Nakai, K. and Sugano, S. DBTSS: DataBase of human Transcriptional Start Sites and full-length cDNAs. Nucleic Acids Res. 30 (2002) 328-331.
15. Sandelin, A., Wasserman, W. W. and Lenhard, B. ConSite: web-based prediction of regulatory elements using cross-species comparison. Nucleic Acids Res. 32 (2004) W249-252.
16. Mayor, C., Brudno, M., Schwartz, J. R., Poliakov, A., Rubin, E. M., Frazer, K. A., Pachter, L. S. and Dubchak, I. VISTA: visualizing global DNA sequence alignments of arbitrary length. Bioinformatics16 (2000) 1046-1047.
17. Zhu, J., He, F., Hu, S. and Yu, J. On the nature of human housekeeping genes. Trends Genet. 24 (2008) 481-484.
18. Fisher, S., Grice, E. A., Vinton, R. M., Bessling, S. L. and McCallion, A. S. Conservation of RET regulatory function from human to zebrafish without sequence similarity. Science312 (2006) 276-279.
19. Waterston, R. H., Lindblad-Toh, K., Birney, E., Rogers, J., Abril, J. F., Agarwal, P., Agarwala, R., Ainscough, R., Alexandersson, M., An, P., Antonarakis, S. E., Attwood, J., Baertsch, R., Bailey, J., Barlow, K., Beck, S., Berry, E., Birren, B., Bloom, T., Bork, P., Botcherby, M., Bray, N., Brent, M. R., Brown, D. G., Brown, S. D., Bult, C., Burton, J., Butler, J., Campbell, R. D., Carninci, P., Cawley, S., Chiaromonte, F., Chinwalla, A. T., Church, D. M., Clamp, M., Clee, C., Collins, F. S., Cook, L. L., Copley, R. R., Coulson, A., Couronne, O., Cuff, J., Curwen, V., Cutts, T., Daly, M., David, R., Davies, J., Delehaunty, K. D., Deri, J., Dermitzakis, E. T., Dewey, C., Dickens, N. J., Diekhans, M., Dodge, S., Dubchak, I., Dunn, D. M., Eddy, S. R., Elnitski, L., Emes, R. D., Eswara, P., Eyras, E., Felsenfeld, A., Fewell, G. A., Flicek, P., Foley, K., Frankel, W. N., Fulton, L. A., Fulton, R. S., Furey, T. S., Gage, D., Gibbs, R. A., Glusman, G., Gnerre, S., Goldman, N., Goodstadt, L., Grafham, D., Graves, T. A., Green, E. D., Gregory, S., Guigo, R., Guyer, M., Hardison, R. C., Haussler, D., Hayashizaki, Y., Hillier, L. W., Hinrichs, A., Hlavina, W., Holzer, T., Hsu, F., Hua, A., Hubbard, T., Hunt, A., Jackson, I., Jaffe, D. B., Johnson, L. S., Jones, M., Jones, T. A., Joy, A., Kamal, M., Karlsson, E. K., Karolchik, D., Kasprzyk, A., Kawai, J., Keibler, E., Kells, C., Kent, W. J., Kirby, A., Kolbe, D. L., Korf, I., Kucherlapati, R. S., Kulbokas, E. J., Kulp, D., Landers, T., Leger, J. P., Leonard, S., Letunic, I., Levine, R., Li, J., Li, M., Lloyd, C., Lucas, S., Ma, B., Maglott, D. R., Mardis, E. R., Matthews, L., Mauceli, E., Mayer, J. H., McCarthy, M., McCombie, W. R., McLaren, S., McLay, K., McPherson, J. D., Meldrim, J., Meredith, B., Mesirov, J. P., Miller, W., Miner, T. L., Mongin, E., Montgomery, K. T., Morgan, M., Mott, R., Mullikin, J. C., Muzny, D. M., Nash, W. E., Nelson, J. O., Nhan, M. N., Nicol, R., Ning, Z., Nusbaum, C., O'Connor, M. J., Okazaki, Y., Oliver, K., Overton-Larty, E., Pachter, L., Parra, G., Pepin, K. H., Peterson, J., Pevzner, P., Plumb, R., Pohl, C. S., Poliakov, A., Ponce, T. C., Ponting, C. P., Potter, S., Quail, M., Reymond, A., Roe, B. A., Roskin, K. M., Rubin, E. M., Rust, A. G., Santos, R., Sapojnikov, V., Schultz, B., Schultz, J., Schwartz, M. S., Schwartz, S., Scott, C., Seaman, S., Searle, S., Sharpe, T., Sheridan, A., Shownkeen, R., Sims, S., Singer, J. B., Slater, G., Smit, A., Smith, D. R., Spencer, B., Stabenau, A., Stange-Thomann, N., Sugnet, C., Suyama, M., Tesler, G., Thompson, J., Torrents, D., Trevaskis, E., Tromp, J., Ucla, C., Ureta-Vidal, A., Vinson, J. P., Von Niederhausern, A. C., Wade, C. M., Wall, M., Weber, R. J., Weiss, R. B., Wendl, M. C., West, A. P., Wetterstrand, K., Wheeler, R., Whelan, S., Wierzbowski, J., Willey, D., Williams, S., Wilson, R. K., Winter, E., Worley, K. C., Wyman, D., Yang, S., Yang, S. P., Zdobnov, E. M., Zody, M. C. and Lander, E. S. Initial sequencing and comparative analysis of the mouse genome. Nature420 (2002) 520-562.
20. Smale, S. T. and Kadonaga, J. T. The RNA polymerase II core promoter. Annu. Rev. Biochem. 72 (2003) 449-479.
21. Kadonaga, J. T., Carner, K. R., Masiarz, F. R. and Tjian, R. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell51 (1987) 1079-1090.