Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water

Applied and Environmental Microbiology

Volumn 78, Issue 1, 2012, Pages 156-162

  Johnson, Anne M ^a   Di Giovanni, George D ^b,c   Rochelle, Paul A ^a  

^a METROPOLITAN WATER DISTRICT OF SOUTHERN CALIFORNIA   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

^c UNIVERSITY OF TEXAS SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

96-WELL PLATE; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM PARVUM OOCYSTS; DETECTION ASSAY; FALSE POSITIVE; IMMUNOFLUORESCENT ANTIBODIES; LOW DOSE; OOCYSTS; PCR ASSAY; REVERSE TRANSCRIPTASES; SENSITIVE DETECTION;

ASSAYS; CELLS; FLOW CYTOMETRY; MONOLAYERS; POLYMERASE CHAIN REACTION; POTABLE WATER; WATER FILTRATION; WATER TREATMENT;

CELL CULTURE;

DRINKING WATER;

BIOASSAY; DRINKING WATER; FILTRATION; FLOW CYTOMETRY; INFECTIVITY; POLYMERASE CHAIN REACTION; PROTOZOAN; REAL TIME; WATER TREATMENT;

ARTICLE; COMPARATIVE STUDY; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; ENVIRONMENTAL MONITORING; EVALUATION; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; OOCYST; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR CELL LINE;

CELL LINE, TUMOR; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; DRINKING WATER; ENVIRONMENTAL MONITORING; FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; OOCYSTS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER MICROBIOLOGY;

CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SP.;

EID: 84855709355     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.06444-11     Document Type: Article

References (29)

1. Aboytes A, et al. 2004. Detection of infectious Cryptosporidium in filtered drinking water. J. Am. Water Works Assoc. 96:88-98.
2. Bukhari Z, Holt DM, Ware MW, Schaefer FW, III. 2007. Blind trials evaluating in vitro infectivity of Cryptosporidium oocysts using cell culture immunofluorescence. Can. J. Microbiol. 53:656-663.
3. Bukhari Z, LeChevallier M. 2003. Assessing UV reactor performance for treatment of finished water. Water Sci. Technol. 47:179-184.
4. Clancy JL, McCuin RM, Hargy TM. 2003. Recovery of Cryptosporidium oocysts from high-volume water samples. American Water Works Association, Denver, CO.
5. Di Giovanni GD, et al. 1999. Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture PCR. Appl. Environ. Microbiol. 65:3427-3432.
6. Di Giovanni GD, LeChevallier MW. 2005. Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection. Appl. Environ. Microbiol. 71:1495-1500.
7. Garvey M, Farrell H, Cormican M, Rowan N. 2010. Investigations of the relationship between use of in vitro culture-quantitative PCR and a mouse-based bioassay for evaluating critical factors affecting the disinfection performance of pulsed UV light for treating Cryptosporidium parvum oocysts in saline. J. Microbiol. Methods 80:267-273.
8. Gennaccaro AL, McLaughlin MR, Wuintero-Betancourt W, Huffman DE, Rose JB. 2003. Infectious Cryptosporidium parvum oocysts in final reclaimed effluent. Appl. Environ. Microbiol. 69:4983-4984.
9. Joachim A, Eckert E, Petry F, Bialek R, Daugschies A. 2003. Comparison of viability assays for Cryptosporidium parvum oocysts after disinfection. Vet. Parasitol. 111:47-57.
10. Johnson AM, et al. 2005. UV inactivation of Cryptosporidium hominis as measured in cell culture. Appl. Environ. Microbiol. 71:2800-2802.
11. Keegan A, Daminato D, Saint CP, Monis PT. 2008. Effect of water treatment processes on Cryptosporidium infectivity. Water Res. 42: 1805-1811.
12. Keegan AR, Fanok S, Monis PT, Saint CP. 2003. Cell culture-TaqMan PCR assay for evaluation of Cryptosporidium parvum disinfection. Appl. Environ. Microbiol. 69:2505-2511.
13. Lalancette C, Di Giovanni GD, Prevost M. 2010. Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay. Appl. Environ. Microbiol. 76:566-577.
14. LeChevallier MW, et al. 2003. Comparison of Method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters. Appl. Environ. Microbiol. 69:971-979.
15. MacKenzie WR, et al. 1994. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. N. Engl. J. Med. 331:161-167.
16. Nichols RAB, Conelly L, Sullivan CB, Smith HV. 2010. Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period. Appl. Environ. Microbiol. 76: 5977-5986.
17. Quintero-Betancourt W, Gennaccaro AL, Scott TM, Rose JB. 2003. Assessment of methods for detection of infectious Cryptosporidium oocysts and Giardia cysts in reclaimed effluents. Appl. Environ. Microbiol. 69:5380-5388.
18. Rochelle PA, et al. 2004. Irreversible UV inactivation of Cryptosporidium spp. despite the presence of UV repair genes. J. Eukaryot. Microbiol. 51: 553-562.
19. Rochelle PA, et al. 1997. An assay combining cell culture with reverse transcriptase-PCR to detect and determine infectivity of waterborne Cryptosporidium parvum. Appl. Environ. Microbiol. 63:2029-2037.
20. Rochelle PA, Ferguson DM, Johnson AM, De Leon R. 2001. Quantitation of Cryptosporidium parvum infection in cell culture using a colorimetric in situ hybridization assay. J. Eukaryot. Microbiol. 48:565-574.
21. Rochelle PA, et al. 2002. Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum. Appl. Environ. Microbiol. 68:3809-3817.
22. Sifuentes LY, Di Giovanni GD. 2007. Aged HCT-8 cell monolayers support Cryptosporidium parvum infection. Appl. Environ. Microbiol. 73: 7548-7551.
23. Slifko TR, Huffman DE, Rose JB. 1999. A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 65:3936-3941.
24. Slifko TR, et al. 2002. Comparison of tissue culture and animal models for assessment of Cryptosporidium parvum infection. Exp. Parasitol. 101: 97-106.
25. Upton SJ, Tilley M, Brillhart DB. 1995. Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT-8 cells. J. Clin. Microbiol. 33:371-375.
26. U.S. Environmental Protection Agency. 2005. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. EPA 815-R-05-002. Office of Research and Development, Government Printing Office, Washington, DC.
27. Woods KM, Nesterenko MV, Upton SJ. 1995. Development of a microtitre ELISA to quantify development of Cryptosporidium parvum in vitro. FEMS Microbiol. Lett. 128:89-94.
28. Xiao L. 2010. Molecular epidemiology of cryptosporidiosis: an update. Exp. Parasitol. 124:80-89.
29. Xiao L, Ryan UM. 2008. Molecular epidemiology, p 119-163. In Fayer R (ed.), Cryptosporidium and cryptosporidiosis. CRC Press, Boca Raton, FL.