Encapsulation-dehydration method elevates embryogenic abilities of Gentiana kurroo cell suspension and carrying on genetic stability of its regenerants after cryopreservation

Acta Horticulturae

Volumn 908, Issue , 2011, Pages 143-154

  Mikula, A ^a   Tomiczak, K ^a   Wojcik A ^a   Rybczynski, J J ^a  

^a POLISH ACADEMY OF SCIENCES   (Poland)

Author keywords

Cryopreservation; Flow cytometry; Gentian; metAFLP; Nuclear DNA content; Regenerants; Somatic embryogenesis

Indexed keywords

GENTIANA; GENTIANA KURROO;

EID: 84855696223     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2011.908.16     Document Type: Conference Paper

References (35)

1. Aguilar, M.E., Engelmann, F. and Michaux-Ferrière, N. 1993. Cryopreservation of cell suspensions of Citrus deliciosa Tan. and histological study. CryoLetters 14:217-228.
2. Bachiri, Y., Gazeau, C., Hansz J., Morisset, C. and Dereuddre, J. 1995. Successful cryopreservation of suspension cells by encapsulation-dehydration. Plant Cell Tiss. Org. Cult. 43:241-248. (Pubitemid 2614994)
3. Bednarek, P., Orłowska, R., Koebner, R.M.D. and Zimny, J. 2007. Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.). BMC Plant Biol. 7:10.
4. Dixit, S., Mandal, B.B., Ahuja, S. and Srivastava, P.S. 2003. Genetic stability assessment of plant regenerated from cryopreserved embryogenic tissue of Discorea bulbifera L. using RAPD, Biochemical and morphological analysis. CryoLetters 24:77-84. (Pubitemid 36647137)
5. Fiuk, A. and Rybczyński, J.J. 2007. The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle). Plant Cell Tiss. Org. Cult. 91:263-271. (Pubitemid 47632213)
6. Fiuk, A. and Rybczyński, J.J. 2008a. Genotype and plant growth regulator-dependent response of somatic embryogenesis from Gentiana sp. leaf explant. In Vitro Cell. Dev. Biol. Planta 44:90-99.
7. Fiuk, A. and Rybczyński, J.J. 2008b. Morphogenic capability of Gentiana kurroo Royle seedling and leaf explants. Acta Physiol. Plant. 30:157-166. (Pubitemid 351199095)
8. Fiuk, A. and Rybczyński, J.J. 2008c. Factor influencing efficiency of somatic embryogenesis of Gentiana kurroo (Royle) cell suspension. Plant Biotechnol. Rep. 2:33-39.
9. Fiuk, A., Bednarek, P. and Rybczyński, J.J. 2010. Flow cytometry, HPLC-RP and metALFP analyses to assess genetic variability in somatic embryo-derived plantlets of Gentiana pannonica Scop. Plant Mol. Biol. Rep. 28:413-420.
10. Hao, Y.J., Liu, Q.L. and Deng, X.X. 2001. Effect of cryopreservation on apple genetic resources at morphological, chromosomal and molecular levels. Cryobiol. 43:64-53.
11. Harding, K. 2004. Genetic integrity of cyopreseved plant cells. A review. CryoLetters 25:2-22.
12. Helliot, B., Madur, D., Dirlewancer, E. and De Boucaud, M.T. 2002. Evaluation of genetic stability in cryopreserved Prunus. In Vitro Cell. Dev. Biol.-Plant. 38:493-500. (Pubitemid 35293930)
13. Hosokawa, K., Matsuki, R., Oikawa, Y. and Yamamura, S. 1997. Genetic transformation of Gentiana using wild-type Agrobacterium rhizogenes. Plant Cell Tiss. Org. Cult. 51:137-140. (Pubitemid 28107225)
14. Kaity, A., Ashmore, S.E., Drew, R.A. and Dulloo, M.E. 2008. Assessment of genetic and epigenetic changes following cryopreservation in papaya. Plant Cell Rep. 27:1529-1539.
15. Martin, C. and Gonzales-Benito, M.E. 2005. Survival and genetic stability of Dendranthema grandiflora Tzvelv shoot apices after cryopreservation by vitrification and encapsulation-dehydration. Cryobiology 51:281-289. (Pubitemid 41758537)
16. Mikuła, A. 2006. Comparison of three techniques for cryopreservation and reestablishment of long-term Gentiana tibetica suspension culture. CryoLetters 27:269-282. (Pubitemid 44951669)
17. Mikuła, A., Olas, M., Sliwińska, E. and Rybczyński, J.J. 2008. Cryopreservation by encapsulation of Gentiana spp. cell suspensions maintains regrowth, embryogenic competence and DNA content. CryoLetters 29:409-418.
18. Mikuła, A., Skierski, J. and Rybczyński, J.J. 2002. Somatic embryogenesis of Gentiana genus. III. Characterization of three-years-old embryogenic suspensions of G. pannonica originated from various seedling explants. Acta Physiol. Plant. 24:311-322. (Pubitemid 35211968)
19. Mikuła, A., Tykarska, T. and Kuraś, M. 2005. Ultrustructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters 26:376-378.
20. Momcilovic, I., Grubisić, D., Kojić, M. and Nesković, M. 1997. Agrobacterium rhozogenes - mediated transformation and plant regeneration of four Gentiana species. Plant Cell Tiss. Org. Cult. 50:1-6. (Pubitemid 27506547)
21. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473-479.
22. Rybczyński, J.J., Borkowska, B., Fiuk, A., Gawrońska, H., Śliwińska, E. and Mikuła, A. 2007. Effect of sucrose concentration on photosynthetic activity of in vitro culture Gentiana kurroo (Royle) germlings. Acta Physiol. Plant. 29:445-453. (Pubitemid 47383223)
23. Scocchi, A., Faloci, M., Medina, R., Olmos, S. and Mroginski, L. 2004. Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydratation and assessment of their genetic stability. Euphytica 135:29-38. (Pubitemid 38154269)
24. Suzuki, M., Akihama, T. and Ishikawa, M. 2005. Cryopreservation of encapsulated Gentiana axillary buds following two step-preculture with sucrose and desiccation. Plant Cell Tiss. Org. Cult. 83:115-121. (Pubitemid 41187473)
25. Suzuki, M., Ishikawa, M. and Akihama, T. 1998. A novel preculture metod for the induction of desiccation tolerance in gentian axillary buds for cryopreservation. Plant Sci. 135:69-76. (Pubitemid 28340498)
26. Tanaka, D., Niino, T., Isuzugawa, K., Hikage, T. and Lemura, M. 2004. Cryopreservation of shoot apices of in vitro grown gentian plants: comparison of vitrification and encapsulation-vitrification protocols. CryoLetters 25:167-176. (Pubitemid 38852045)
27. Turner, S., Krauss, S.L., Bunn, E., Senaratna, T., Dixon, K., Tan, B. and Touchell, D. 2001. Genetic fidelity and variability of Anigozantos viridis following tissue culture, cold storage and cryopreservation. Plant Sci. 161:1099-1106. (Pubitemid 34003145)
28. Wang, Z.Y., Legris, G., Nagel, J., Potrykus, I. and Spangenberg, G. 1994. Cryopreservation of embryogenic cell suspensions in Festuca and Lolium species. Plant Scie. 103:93-106.
29. Winkelmann, T., Mußmann, V. and Serek, M. 2004. Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill. Plant Cell Rep. 23:1-8. (Pubitemid 39123138)
30. Vinterhalter, B., Orbovic, V. and Vinterhalter, D. 1999. Transgenic root cultures of Gentiana punctata L. Acta Soc. Bot. Pol. 68:275-280. (Pubitemid 129483313)
31. Wang, Q., Gafny, R., Sahar, N., Sela, I., Mawassi, M., Tanne, E. and Perl, A. 2002. Cyopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Sci. 162:551-558. (Pubitemid 34546682)
32. Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23:4407-4414.
33. Xue, S.H., Luo, X.J., Wu, Z.H., Hang, H.L. and Wang, X.Y. 2008. Cold storage and cryopreservation of hairy roots cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall. Plant Cell Tiss. Org. Cult. 92:251-260.
34. Zarghami, R., Pirseyedi, M., Hasrak, S. and Sardrood, B.P. 2008. Evaluation of genetic stability in cryopreserved Solanum tuberosum. African Journal of Biotechnology. 7:2798-2802. http://academicjournals.org/AJB.
35. Zhai, Z., Engelmann, F., Chen, R. and Zhao, Y. 2003. Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. Cryoletters 24:315-322. (Pubitemid 37387902)