Flow cytometry, HPLC-RP, and metAFLP analyses to assess genetic variability in somatic embryo-derived plantlets of Gentiana pannonica Scop

Plant Molecular Biology Reporter

Volumn 28, Issue 3, 2010, Pages 413-420

  Fiuk, Agnieszka ^a   Bednarek, Piotr T ^b   Rybczyński, Jan J ^a  

^a POLISH ACADEMY OF SCIENCES   (Poland)

^b PLANT BREEDING AND ACCLIMATIZATION INSTITUTE NATIONAL RESEARCH INSTITUTE   (Poland)

Author keywords

DNA methylation; Genetic stability; Gentiana; Somatic embryogenesis

Indexed keywords

GENTIANA; GENTIANA PANNONICA;

EID: 77952092141     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-009-0167-3     Document Type: Article

References (41)

1. Baurens FC, Bonnot F, Bienvenu S, Causse S, Lagavre T (2003) Using SD-AFLP and MSAP to assess CCGG methylation in banana genome. Plant Mol Biol Rep 21: 339-348.
2. Bednarek PT, Orłowska R, Koebner RMD, Zimny J (2007) Quantification of tissue-culture induced variation in barley (Hordeum vulgare L.). BMC Plant Biology 7: 10.
3. Cervera M, Ruiz-Garcia L, Martinez-Zapater J (2002) Analysis of DNA methylation in Arabidopsis thaliana based on methylation-sensitive AFLP markers. Mol Genet Genomics 268: 543-552.
4. Chowdhury MKU, Vasil IK (1992) Molecular analysis of plants regenerated from embryogenic cultures of hybrid sugarcane cultivars (Saccharum spp.). Theor Appl Genet 86: 181-188.
5. Fiuk A, Rajkiewicz M, Rybczyński JJ (2003) Gentiana kurroo (Royle) in in vitro culture. Biotechnology 3: 267-274.
6. Fraś A, Małuszyńska J (2004) The correlation between the chromosome variation in callus and genotype of explants of Arabidopsis thaliana. Genetica 121: 145-154.
7. Grayburn WS, Selker EU (1989) A natural case of RIP: degeneration of the DNA sequence in an ancestral tandem duplication. Mol Cell Biol 9(10): 4416-4421.
8. Guo WL, Gong L, Ding ZF, Li YD, Li FX, Zhao SP, Liu B (2006) Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f. as revealed by ISSR and RAPD markers. Plant Cell Rep 25(9): 896-906.
9. Guzy-Wróblewska J, Szarejko I (2003) Molecular and agronomic evaluation of wheat double haploid lines obtained through maize pollination and anther methods. Plant Breeding 122: 305-313.
10. Hornero J, Martínez I, Celestyno C, Gallego FJ, Torres V, Toribio M (2001) Early checking of genetic stability of cork oak somatic embryos by AFLP analysis. Int J Plant Sci 162: 827-833.
11. Jaligot E, Rival A, Beulé T, Dussert S, Verdeil JL (2000) Somaclonal variation in oil palm (Elaeis guineensis Jacq.): the DNA methylation hypothesis. Plant Cell Rep 19: 684-690.
12. Jaligot E, Beulé T, Rival A (2002) Methylation-sensitive RFLPs: characterisation of two oil palm markers showing somaclonal variation-associated polymorphism. Theor Appl Genet 104: 1263-1269.
13. Johnston JW, Harding K, Bremner DH, Souch G, Green J, Lynch PT, Grout B, Benson EE (2005) HPLC analysis of plant DNA methylation: a study of critical methodological factors. Plant Physiol Bioch 43: 844-853.
14. Kaeppler SM, Phillips RL (1993) Tissue culture-induced DNA methylation variation in maize. Proc Natl Acad Sci USA 90: 8773-8776.
15. Kaeppler SM, Phillips RL, Olhoft P (1998) Molecular basis of heritable tissue culture-induced variation in plants. In: Jain SM Brar DS Ahloowalia BS (eds.) Somaclonal variation and Induced Mutations in Crop Improvement. Kluwer Academic Publishers Dordrecht Printed in Great Britain pp 465-484.
16. Kononowicz H (1990) In vitro plant embryogenesis. Biotechnology 2(3): 52-59.
17. Kumar PS, Mathur VL (2004) Chromosomal instability in callus culture of Pisum sativum. Plant Cell Tiss Org Cult 78: 267-271.
18. Larkin PJ, Scowcroft WR (1981) Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197-214.
19. Linacero R, Freitas AE, Vázquez AM (2000) Hot spots of DNA instability revealed through the study of somaclonal variation in rye. Theor Appl Genet 3(4): 506-511.
20. Littell R, Stroup W, Freund R (2002) SAS for linear models fourth edition, 4th edn. SAS Publishing SAS Institute Inc, Cary.
21. LoSchiavo F, Pitto L, Giuliano G, Tori G, Nuti-Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M (1989) DNA methylation of embryonic carrot cell cultures and its variations as caused by mutation differentiation hormones and hypomethylating drugs. Theor Appl Genet 77: 325-331.
22. Matthes M, Singh R, Cheah SC, Karp A (2001) Variation in oil palm (Elaeis guineensis Jacq) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes. Theor Appl Genet 102: 971-979.
23. Mikuła A, Tykarska T, Kuraś M, Iwanowska A, Rybczyński JJ (1996) Studies on the morphogenic potential of gentians in cell suspension. Symposium Breeding Research on Medicinal and Aromatic Plants. Springer Quedlinburg Germany pp 290-295.
24. Mikuła A, Rybczyński JJ (2001) Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.) G. pannonica (Scop.) and G. tibetica (King). Acta Physiol Plant 23: 15-25.
25. Mukhopadhyay MJ, Sengupta P, Mukhopadhyay S, Sen S (2005) In vitro stable regeneration of onion and garlic from suspension culture and chromosomal instability in solid callus culture. Sci Hort 104: 1-9.
26. Müller E, Brown PTH, Hartke S, Lörz H (1990) DNA variation in tissue culture derived rice plants. Theor Appl Genet 80: 673-679.
27. Munthali MT, Newbury HJ, Ford-Lloyd BV (1996) The detection of somaclonal variants of beet using RAPD. Plant Cell Rep 15: 474-478.
28. Oleszczuk S, Zimny J, Bednarek PT (2002) The application of the AFLP method to determine the purity of homozygous lines of barley (Hordeum vulgare L.). Cell Mol Biol Lett 7: 777-783.
29. Patnaik J, Sahoo S, Debata BK (1997) Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa Grass (Cymbopogon martinii). Plant Cell Rep 16: 430-434.
30. Peraza-Echeverria S, Herrera-Valencia VA, James-Kay A (2001) Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP). Plant Sci 161: 359-367.
31. Pontaroli AC, Camardo EL (2005) Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures. Genet Mol Biol 28(3): 423-430.
32. Portis E, Acquadro A, Comino C, Lanteri S (2003) Analysis of DNA methylation during germination of pepper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP). Plant Sci 166: 169-178.
33. Rani V, Singh KP, Shiran B, Nandy S, Devarumath GS, RM SHL, Raina SN (2000) Evidence for new nuclear and mitochondrial genome organizations among high-frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae). Plant Cell Rep 19: 1013-1020.
34. Rodriguez Lopez CM, Wetten AC, Wilkinson MJ (2004) Detection and quantification of in vitro-culture induced chimerism using simple sequence repeat (SSR) analysis in Theobroma cacao (L.). Theor Appl Genet 110(1): 157-166.
35. Sabała I, Orlikowska T (1993) Possibility of use of somaclonal variation in ornamental plant breeding. Scient Lett of Research Institute of Pomology and Floriculture 1: 41-57.
36. Sabir A, Newbury HJ, Todd G, Catty G, Ford-Lloyd BV (1992) Determination of genetic stability using isoenzymes and RFLPs in plants regenerated in vitro. Theor Appl Genet 84: 113-117.
37. Shen X, Chen J, Kane M, Henny RJ (2007) Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell Tiss Org Cult 91: 21-27.
38. Soniya EV, Banerjee NS, Das MR (2001) Genetic analysis of somaclonal variation among callus-derived plants of tomato. Curr Sci 80(9): 1213-1215.
39. Thomas J, Vijayan D, Joshi SD, Joseph Lopez S, Raj Kumar R (2006) Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats. J Biotechnol 123(2): 149-154.
40. Vendrame WA, Kochert G, Wetzstein HY (1999) AFLP analysis of variation in pecan somatic embryos. Plant Cell Rep 18: 853-857.
41. Xiong LZ, Xu CG (1999) MA, Zhang Q. Patterns of cytosine methylation in an elite rice hybrid and its parental lines detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet 261: 439-446.