Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples

FEMS Immunology and Medical Microbiology

Volumn 64, Issue 1, 2012, Pages 92-97

  Giulieri, Stefano ^a   Jaton, Katia ^a   Cometta, Alain ^b   Trellu, Laurence T ^c   Greub, Gilbert ^a  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^b Centre Hospitalier Yverdon Chamblon   (Switzerland)

^c UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

Polymerase chain reaction; Rickettsia; Spotted fever; Typhus

Indexed keywords

CITRATE SYNTHASE; RNA 16S;

ARTICLE; CONTROLLED STUDY; HUMAN; NONHUMAN; PETECHIA; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RICKETTSIA PROWAZEKII; RICKETTSIA TYPHI; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEROCONVERSION; SKIN BIOPSY; TYPHUS;

ADULT; BACTERIAL PROTEINS; CITRATE (SI)-SYNTHASE; DNA PRIMERS; FEMALE; HUMANS; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; RICKETTSIA; RICKETTSIA INFECTIONS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 84855692370     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2011.00910.x     Document Type: Article

References (16)

1. Bechah Y, Capo C, Mege JL & Raoult D (2008) Epidemic typhus. Lancet Infect Dis 8: 417-426.
2. Boillat N, Genton B, D'Acremont V, Raoult D & Greub G (2008) Fatal case of Israeli spotted fever after Mediterranean cruise. Emerg Infect Dis 14: 1944-1946.
3. Brouqui P, Bacellar F, Baranton G et al. (2004) Guidelines for the diagnosis of tick-borne bacterial diseases in Europe. Clin Microbiol Infect 10: 1108-1132.
4. Choi YJ, Lee SH, Park KH et al. (2005) Evaluation of PCR-based assay for diagnosis of spotted fever group rickettsiosis in human serum samples. Clin Diagn Lab Immunol 12: 759-763.
5. Dumler JS, Taylor JP & Walker DH (1991) Clinical and laboratory features of murine typhus in south Texas, 1980 through 1987. JAMA 266: 1365-1370.
6. Fournier PE & Raoult D (2004) Suicide PCR on skin biopsy specimens for diagnosis of rickettsioses. J Clin Microbiol 42: 3428-3434.
7. Fournier PE, Jensenius M, Laferl H, Vene S & Raoult D (2002) Kinetics of antibody responses in Rickettsia africae and Rickettsia conorii infections. Clin Diagn Lab Immunol 9: 324-328.
8. Fournier PE, Dumler JS, Greub G, Zhang J, Wu Y & Raoult D (2003) Gene sequence-based criteria for identification of new rickettsia isolates and description of Rickettsia heilongjiangensis sp. nov. J Clin Microbiol 41: 5456-5465.
9. Karpathy SE, Hayes EK, Williams AM et al. (2009) Detection of Rickettsia felis and Rickettsia typhi in an area of California endemic for murine typhus. Clin Microbiol Infect 15(Suppl 2): 218-219.
10. Leitner M, Yitzhaki S, Rzotkiewicz S & Keysary A (2002) Polymerase chain reaction-based diagnosis of Mediterranean spotted fever in serum and tissue samples. Am J Trop Med Hyg 67: 166-169.
11. Paris DH, Blacksell SD, Stenos J et al. (2008) Real-time multiplex PCR assay for detection and differentiation of rickettsiae and orientiae. Trans R Soc Trop Med Hyg 102: 186-193.
12. Parola P, Paddock CD & Raoult D (2005) Tick-borne rickettsioses around the world: emerging diseases challenging old concepts. Clin Microbiol Rev 18: 719-756.
13. Roux V, Rydkina E, Eremeeva M & Raoult D (1997) Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. Int J Syst Bacteriol 47: 252-261.
14. Rozen S & Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365-386.
15. Tzianabos T, Anderson BE & McDade JE (1989) Detection of Rickettsia rickettsii DNA in clinical specimens by using polymerase chain reaction technology. J Clin Microbiol 27: 2866-2868.
16. Walker DH, Valbuena GA & Olano JP (2003) Pathogenic mechanisms of diseases caused by Rickettsia. Ann NY Acad Sci 990: 1-11.