Branched polyethylene glycol for protein precipitation

Biotechnology and Bioengineering

Volumn 109, Issue 3, 2012, Pages 736-746

  Sim, Siow Leng ^a,b   He, Tao ^c   Tscheliessnig, Anne ^a,d   Mueller, Monika ^a   Tan, Reginald B H ^b,c   Jungbauer, Alois ^d  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c INSTITUTE OF CHEMICAL AND ENGINEERING SCIENCES   (Singapore)

^d UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Branched; Hydrodynamic radius; Polyethylene glycol; Precipitation; Protein; Viscosity

Indexed keywords

BRANCHED; BRANCHED POLYETHYLENES; BRANCHED STRUCTURES; DYNAMIC VISCOSITIES; HIGH VISCOSITIES; HYDRODYNAMIC RADIUS; IGG RECOVERY; PRECIPITATION KINETICS; PROTEIN PRECIPITATION; PURIFIED PROTEIN; REDUCED DYNAMICS; STERIC MECHANISM; VOLUME EXCLUSION;

CELL CULTURE; GLYCOLS; HYDRODYNAMICS; MOLECULAR BIOLOGY; MOLECULAR WEIGHT; POLYETHYLENES; PRECIPITATION (CHEMICAL); PROTEINS; STARS; THERMOPLASTICS; VISCOSITY;

POLYETHYLENE GLYCOLS;

IMMUNOGLOBULIN G; MACROGOL;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHEMICAL STRUCTURE; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; HYDRODYNAMICS; IMMUNOPRECIPITATION; MOLECULAR WEIGHT; NONHUMAN; POLYMERIZATION; PRECIPITATION; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN PURIFICATION; SOLUBILITY; SUPERNATANT; SYNTHESIS; VISCOSITY;

ANIMALS; BIOTECHNOLOGY; CHEMICAL PRECIPITATION; CHO CELLS; CRICETINAE; CRICETULUS; IMMUNOGLOBULIN G; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; RECOMBINANT PROTEINS;

EID: 84855683807     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24343     Document Type: Article

References (21)

1. Asakura S, Oosawa F. 1958. Interaction between particles suspended in solutions of macromolecule. J Polym Sci 33: 183-192.
2. Atha DH, Ingham KC. 1981. Mechanism of precipitation of proteins by polyethylene glycols. Analysis in terms of excluded volume. J Biol Chem 256(23): 12108-12117.
3. Bell DJ, Hoare M, Dunhill P. 1983. The formation of protein precipitates and their centrifugal recovery. In: Fitcher A, editor. Advances in biochemical engineering/biotechnology 26. New York: Springer-Verlag. p 1-72.
4. Bergstrom K, Osterberg E, Holmberg K, Hoffman AS, Schuman TP, Kozlowski A, Harris JM. 1994. Effect of branching and molecular weight of surface-bound poly(ethylene oxide) on protein rejection. J Biomater Sci Polym Ed 6: 123-132.
5. Bulte JW, Douglas T, Mann S, Frankel RB, Moskowitz BM, Brooks RA, Baumgarner CD, Vymazal J, Strub MP, Frank JA. 1994. Magnetoferritin: Characterization of a novel superparamagnetic MR contrast agent. J Magn Reson Imaging 4: 497-505.
6. Choo AB, Tan HL, Ang SN, Fong WJ, Chin A, Lo J, Zheng L, Hentze H, Philip RJ, Oh SK, Yap M. 2008. Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1. Stem Cells 26: 1454-1463.
7. Dohmen MPJ, Pereira AM, Timmer JMK, Benes NE, Keurentjes JTF. 2008. Hydrodynamic radii of polyethylene glycols in different solvents determined from viscosity measurements. J Chem Eng Data 53: 63-65.
8. Flory PJ. 1953. Principles of polymer chemistry. New York: Cornell University Press. 309 p.
9. Gnanou Y, Lutz P, Rempp P. 1988. Synthesis of star-shaped poly(ethy1ene oxide). Makromol Chem 189: 2885-2892.
10. Harrison RG, Todd P, Rudge SR, Petrides DP. 2003. Bioseparations science and engineering. New York: Oxford University Press. 250-251, 255 p.
11. Iverius PH, Laurent TC. 1967. Precipitation of some plasma proteins by the addition of dextran or polyethylene glycol. Biochim Biophys Acta 133: 371-373.
12. Juckes IR. 1971. Fractionation of proteins and viruses with polyethylene glycol. Biochim Biophys Acta 229(3): 535-546.
13. Meier MAR, Schubert US. 2003. Evaluation of a new multiple-layer spotting technique for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of synthetic polymers. Rapid Commun Mass Spectrom 17: 713-716.
14. Polson A. 1977. A theory for the displacement of proteins and viruses with polyethylene glycol. Prep Biochem 7(2): 129-159.
15. Polson A, Potgieter GM, Largier JF, Mears GE, Joubert FJ. 1964. The fractionation of protein mixtures by linear polymers of high molecular weight. Biochim Biophys Acta 82: 463-475.
16. Scopes RK. 1994. Protein purification: Principles and practice. 3rd edition. New York: Springer. 93 p.
17. Sim SL, He T, Tscheliessnig A, Mueller M, Tan R, Jungbauer A. 2011. Protein precipitation by polyethylene glycol: A generalized model based on hydrodynamic radius. J Biotechnol in press.
18. Stocking CR. 1956. Precipitation of enzymes during isolation of chloroplasts in Carbowax. Science 123: 1032-1033.
19. Tscheliessnig A, Jungbauer A. 2009. High-performance monolith affinity chromatography for fast quantitation of immunoglobulin G. J Chromatogr A 1216(13): 2676-2682.
20. Tscheliessnig A, Ong D, Lee J, Pan S, Satianegara G, Schriebl K, Choo A, Jungbauer A. 2009. Engineering of a two-step purification strategy for a panel of monoclonal immunoglobulin M directed against undifferentiated human embryonic stem cells. J Chromatogr A 1216: 7851-7864.
21. Wallevik K. 1973. Isoelectric focusing of bovine serum albumin: Influence of binding of carrier ampholytes. Biochim Biophys Acta 322(1): 75-87.