A reversed-phase HPLC fluorimetric method for simultaneous determination of homocysteine-related thiols in different body fluids

Laboratory Medicine

Volumn 42, Issue 11, 2011, Pages 657-662

  Persichilli, Silvia ^a   Gervasoni, Jacopo ^a   Castagnola, Massimo ^a,b   Zuppi, Cecilia ^a   Zappacosta, Bruno ^c  

^a CATHOLIC UNIVERSITY   (Italy)

^b CNR   (Italy)

^c CATHOLIC UNIVERSITY   (Italy)

Author keywords

CSF; Glutathione; Homocysteine; HPLC; Saliva; Thiols; Urine

Indexed keywords

CYSTEINE; CYSTEINYLGLYCINE; GLUTATHIONE; HOMOCYSTEINE; SULFUR;

ADULT; ARTICLE; BLOOD ANALYSIS; BODY FLUID; CAPILLARY ELECTROPHORESIS; CEREBROSPINAL FLUID; CONTROLLED STUDY; FEMALE; FLUOROMETRY; HUMAN; LIQUID CHROMATOGRAPHY; MALE; MASS SPECTROMETRY; NORMAL HUMAN; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SALIVA ANALYSIS; URINALYSIS;

EID: 84855554802     PISSN: 00075027     EISSN: 19437730     Source Type: Journal    
DOI: 10.1309/LMOIAH19RG5BKBIQ     Document Type: Article

References (23)

1. Mansoor MA, Svardal AM, Ueland PM. Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma. Anal Biochem. 1992;200:218-229.
2. Brosnan JT, Brosnan ME. The sulfur-containing amino acids: An overview. J Nutr. 2006;136:1636S-16340S.
3. Pastore A, Federici G, Bertini E, et al. Analysis of glutathione: Implication in redox and detoxification. Clin Chim Acta. 2003;333:19-39.
4. Mudd SH, Levy HL, Skovby F. Disorders of transsulfuration. In: Scriver CR, Beaudet AL, Sly WS, et al, eds. The Metabolic and Molecular Bases of Inherited Disease. 7th ed. Vol. 1. New York: McGraw-Hill, 1995:1279-327.
5. Araki A, Sako Y. Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection. J Chromatogr. 1987;422:43-52.
6. Zappacosta B, Manni A, Persichilli S, et al. HPLC analysis of some sulphur compounds in saliva: Comparison between healthy subjects and periodontopathic patients. Clin Chim Acta. 2003;338:57-60.
7. Fiskerstrand T, Refsum H, Kvalheim G, et al. Homocysteine and other thiols in plasma and urine: Automated determination and sample stability. Clin Chem. 1993;39:263-271.
8. Pastore A, Massoud R, Motti C, et al. Fully automated assay for total homocysteine, cysteine, cysteinylglycine, glutathione, cysteamine, and 2-mercaptopropionylglycine in plasma and urine. Clin Chem. 1998;44:825-832.
9. Bergmark C, Mansoor MA, Svardal A, et al. Redox status of plasma homocysteine and related aminothiols in smoking and nonsmoking young adults. Clin Chem. 1997;43:1997-1999.
10. Jacobsen DW, Gatautis VJ, Green R, et al. Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: Sex differences and correlation with cobalamin and folate concentrations in healthy subjects. Clin Chem. 1994;40:873-881.
11. Andersson A, Isaksson A, Brattström L, et al. Homocysteine and other thiols determined in plasma by HPLC and thiol-specific postcolumn derivatization. Clin Chem. 1993;39:1590-1597.
12. Kuśmierek K, Głowacki R, Bald E. Analysis of urine for cysteine, cysteinylglycine, and homocysteine by high-performance liquid chromatography. Anal Bioanal Chem. 2006;385:855-860.
13. Lochman P, Adam T, Friedecký D, et al. High-throughput capillary electrophoretic method for determination of total aminothiols in plasma and urine. Electrophoresis. 2003;24:1200-1207.
14. Bald E, Głowacki R. Analysis of saliva for glutathione and metabolically related thiols by liquid chromatography with ultraviolet detection. Amino Acids. 2005;28:431-433.
15. Iwasaki Y, Hoshi M, Ito R, et al. Analysis of glutathione and glutathione disulfide in human saliva using hydrophilic interaction chromatography with mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;839:74-79.
16. Bayir H, Kagan VE, Tyurina YY, et al. Assessment of antioxidant reserves and oxidative stress in cerebrospinal fluid after severe traumatic brain injury in infants and children. Pediatr Res. 2002;51:571-578.
17. Obeid R, Kostopoulos P, Knapp JP, et al. Biomarkers of folate and vitamin B12 are related in blood and cerebrospinal fluid. Clin Chem. 2007;53(2):326-33.
18. Kawakami Y, Monobe M, Kuwabara K, et al. A comparative study of nitric oxide, glutathione, and glutathione peroxidase activities in cerebrospinal fluid from children with convulsive diseases/children with aseptic meningitis. Brain Dev. 2006;28:243-246.
19. Van Hove JL, Lazeyras F, Zeisel SH, et al. One-methyl group metabolism in non-ketotic hyperglycinaemia: Mildly elevated cerebrospinal fluid homocysteine levels. J Inherit Metab Dis. 1998;21:799-811.
20. Hernanz A, Plaza A, Martín-Mola E, et al. Increased plasma levels of homocysteine and other thiol compounds in rheumatoid arthritis women. Clin Biochem. 1999;32:65-70.
21. Zappacosta B, Manni A, Persichilli S, et al. Salivary thiols and enzyme markers of cell damage in periodontal disease. Clin Biochem. 2007;40:661-665.
22. Calabrese V, Scapagnini G, Ravagna A, et al. Disruption of thiol homeostasis and nitrosative stress in the cerebrospinal fluid of patients with active multiple sclerosis: Evidence for a protective role of acetylcarnitine. Neurochem Res. 2003;28:1321-1328.
23. Bayle C, Caussé E, Couderc F. Determination of aminothiols in body fluids, cells, and tissues by capillary electrophoresis. Electrophoresis. 2004;25:1457-1472.