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Volumn 717, Issue , 2011, Pages 55-67

An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry

Author keywords

Nitrocellulose; SDS PAGE; Western blotting

Indexed keywords

ANTIBODY; PROTEIN;

EID: 84855500163     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-024-9_3     Document Type: Chapter
Times cited : (47)

References (51)
  • 1
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to NC sheets: Procedure and applications. Proc
    • Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to NC sheets: procedure and applications. Proc Natl Acad Sci USA. 76, 4350–4354.
    • (1979) Natl Acad Sci USA , vol.76 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3
  • 2
    • 0025572674 scopus 로고
    • Immobilon-P transfer membrane: Applications and utility in protein biochemical analysis
    • Review
    • LeGendre, N. (1990). Immobilon-P transfer membrane: applications and utility in protein biochemical analysis. Biotechniques 9(6 Suppl), 788–805. Review.
    • (1990) Biotechniques , vol.9 , pp. 788-805
    • Legendre, N.1
  • 3
    • 0016700864 scopus 로고
    • Detection of specific sequences among DNA fragments separated by gel electrophoresis
    • Southern, E.M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 98, 503–517.
    • (1975) J Mol Biol , vol.98 , pp. 503-517
    • Southern, E.M.1
  • 4
    • 0017690216 scopus 로고
    • Method for detection of specific RNAs in agar gels by transfer to diazobenzyloxym-ethyl-paper and hybridization with DNA probes
    • Alwine, J.C., and Kemp, D.J., and Stark, G.R. (1977) Method for detection of specific RNAs in agar gels by transfer to diazobenzyloxym-ethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA. 74, 5350–5354.
    • (1977) Proc Natl Acad Sci USA , vol.74 , pp. 5350-5354
    • Alwine, J.C.1    Kemp, D.J.2    Stark, G.R.3
  • 5
    • 0019551730 scopus 로고
    • “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified NC and radiographic detection with antibody and radioiodinated protein A
    • Burnette, W.N. (1981) “Western Blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified NC and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 112, 195–203.
    • (1981) Anal Biochem , vol.112 , pp. 195-203
    • Burnette, W.N.1
  • 6
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during assembly of the head of bacte-riophage T4
    • Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacte-riophage T4. Nature 227, 680–685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 8
    • 0020170968 scopus 로고
    • Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter
    • Gershoni, J.M., and Palade, G.E. (1982) Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal Biochem. 124, 396–405.
    • (1982) Anal Biochem , vol.124 , pp. 396-405
    • Gershoni, J.M.1    Palade, G.E.2
  • 9
    • 0023739598 scopus 로고
    • Protein blotting: A manual
    • Review
    • Gershoni, J.M. (1988) Protein blotting: a manual. Methods Biochem Anal. 33, 1–58. Review.
    • (1988) Methods Biochem Anal , vol.33 , pp. 1-58
    • Gershoni, J.M.1
  • 11
    • 0024498601 scopus 로고
    • Glutaraldehyde fixation increases retention of low molecular weight proteins (growth fac-tors) transferred to nylon membranes for Western blot analysis
    • Karey, K.P., and Sirbasku, D.A. (1989) Glutaraldehyde fixation increases retention of low molecular weight proteins (growth fac-tors) transferred to nylon membranes for Western blot analysis. Anal Biochem. 178, 255–259.
    • (1989) Anal Biochem , vol.178 , pp. 255-259
    • Karey, K.P.1    Sirbasku, D.A.2
  • 13
    • 0010254593 scopus 로고
    • Transfer of proteins from gels to diazobenzy-loxymethyl paper and detection with anti-sera: A method for studying antibody specificity and antigen structure
    • Renart, J., Reiser, J. and Stark, G.R. (1979) Transfer of proteins from gels to diazobenzy-loxymethyl paper and detection with anti-sera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci USA. 76, 3116–3120.
    • (1979) Proc Natl Acad Sci USA , vol.76 , pp. 3116-3120
    • Renart, J.1    Reiser, J.2    Stark, G.R.3
  • 14
    • 0021459963 scopus 로고
    • Blotting intact immunoglobulins and other high-molecular-weight proteins after composite agarose-polyacrylamide gel electrophoresis
    • Elkon, K.B., Jankowski, P.W., and Chu, J.L. (1984) Blotting intact immunoglobulins and other high-molecular-weight proteins after composite agarose-polyacrylamide gel electrophoresis. Anal Biochem. 140, 208–213.
    • (1984) Anal Biochem , vol.140 , pp. 208-213
    • Elkon, K.B.1    Jankowski, P.W.2    Chu, J.L.3
  • 15
    • 0019882091 scopus 로고
    • Protease-facilitated transfer of high-molecular-weight proteins during electrotransfer to NC
    • Gibson, W. (1981). Protease-facilitated transfer of high-molecular-weight proteins during electrotransfer to NC. Anal Biochem. 118, 1–3.
    • (1981) Anal Biochem , vol.118 , pp. 1-3
    • Gibson, W.1
  • 16
    • 0031149725 scopus 로고    scopus 로고
    • High efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    • Bolt, M.W., and Mahoney, P.A. (1997) High efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 247, 185–192.
    • (1997) Anal Biochem , vol.247 , pp. 185-192
    • Bolt, M.W.1    Mahoney, P.A.2
  • 17
    • 0036679941 scopus 로고    scopus 로고
    • Heat mediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to NC membranes
    • Kurien, B.T., and Scofield, R.H. (2002) Heat mediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to NC membranes. J Immunol Methods 266, 127–133.
    • (2002) J Immunol Methods , vol.266 , pp. 127-133
    • Kurien, B.T.1    Scofield, R.H.2
  • 18
    • 0020579390 scopus 로고
    • Protein blotting: Principles and applications
    • Gershoni, J.M., and Palade, G.E. (1983) Protein blotting: principles and applications. Anal Biochem. 131, 1–15.
    • (1983) Anal Biochem , vol.131 , pp. 1-15
    • Gershoni, J.M.1    Palade, G.E.2
  • 19
    • 0030122080 scopus 로고    scopus 로고
    • Identification of glycoproteins on nitrocellulose membranes and gels
    • Thornton, D.J., Carlstedt, I., and Sheehan, J.K. (1996) Identification of glycoproteins on nitrocellulose membranes and gels. Mol Biotechnol. 5, 171–176.
    • (1996) Mol Biotechnol , vol.5 , pp. 171-176
    • Thornton, D.J.1    Carlstedt, I.2    Sheehan, J.K.3
  • 20
    • 0036357175 scopus 로고    scopus 로고
    • NC: A tried and true polymer finds utility as a post-genomic substrate
    • Review
    • Tonkinson, J.L., and Stillman, B. (2002) NC: a tried and true polymer finds utility as a post-genomic substrate. Front Biosci. 7, c1–c12. Review.
    • (2002) Front Biosci , vol.7 , pp. c1-c12
    • Tonkinson, J.L.1    Stillman, B.2
  • 21
    • 0027368709 scopus 로고
    • Peptide dot immunoassay and immunoblotting: Electroblotting from aluminum thin-layer chromatography plates and isoelectric focusing gels to activated NC
    • Lauritzen, E., Masson, M., Rubin, I., Bjerrum, O.J., and Holm, A. (1993) Peptide dot immunoassay and immunoblotting: electroblotting from aluminum thin-layer chromatography plates and isoelectric focusing gels to activated NC. Electrophoresis 14, 852–859.
    • (1993) Electrophoresis , vol.14 , pp. 852-859
    • Lauritzen, E.1    Masson, M.2    Rubin, I.3    Bjerrum, O.J.4    Holm, A.5
  • 22
    • 0027368710 scopus 로고
    • Chemical activation of NC membranes for peptide antigen-antibody binding studies: Direct substitution of the nitrate group with diaminoalkane
    • Masson, M., Lauritzen, E., and Holm, A. (1993) Chemical activation of NC membranes for peptide antigen-antibody binding studies: direct substitution of the nitrate group with diaminoalkane. Electrophoresis 14, 860–865.
    • (1993) Electrophoresis , vol.14 , pp. 860-865
    • Masson, M.1    Lauritzen, E.2    Holm, A.3
  • 23
    • 0028241551 scopus 로고
    • Western blotting of formaldehyde-fixed neuropeptides as small as 400 daltons on gelatin-coated NC paper
    • Too, C.K., Murphy, P.R., and Croll, R.P. (1994) Western blotting of formaldehyde-fixed neuropeptides as small as 400 daltons on gelatin-coated NC paper. Anal Biochem. 219, 341–348.
    • (1994) Anal Biochem , vol.219 , pp. 341-348
    • Too, C.K.1    Murphy, P.R.2    Croll, R.P.3
  • 25
    • 0023664635 scopus 로고
    • Sequence from pico-mole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
    • Matsudaira, P. (1987) Sequence from pico-mole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 262,10035–10038.
    • (1987) J Biol Chem , vol.262 , pp. 10035-10038
    • Matsudaira, P.1
  • 29
    • 0023777627 scopus 로고
    • India ink staining of proteins on nylon and hydrophobic membranes
    • Hughes, J.H., and Mack, K., and Hamparian, V.V. (1988) India ink staining of proteins on nylon and hydrophobic membranes. Anal Biochem. 173, 18–25.
    • (1988) Anal Biochem , vol.173 , pp. 18-25
    • Hughes, J.H.1    Mack, K.2    Hamparian, V.V.3
  • 30
    • 0024460754 scopus 로고
    • Protein binding to NC, nylon and PVDF membranes in immunoassays and electroblotting
    • Tovey, E.R., and Baldo, B.A. (1989) Protein binding to NC, nylon and PVDF membranes in immunoassays and electroblotting. J Biochem Biophys Methods 19, 169–183.
    • (1989) J Biochem Biophys Methods , vol.19 , pp. 169-183
    • Tovey, E.R.1    Baldo, B.A.2
  • 32
    • 0030853836 scopus 로고    scopus 로고
    • Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens
    • Kurien, B.T., and Scofield, R.H. (1997) Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens. J Immunol Methods 205, 91–94.
    • (1997) J Immunol Methods , vol.205 , pp. 91-94
    • Kurien, B.T.1    Scofield, R.H.2
  • 33
    • 0021678736 scopus 로고
    • Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
    • Kyhse-Andersen, J. (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10, 203–209.
    • (1984) J Biochem Biophys Methods , vol.10 , pp. 203-209
    • Kyhse-Andersen, J.1
  • 34
    • 0023198755 scopus 로고
    • A two-step procedure for efficient electro transfer of both high-molecular weight (greater than 400,000) and low-molecular weight (less than 20,000) proteins
    • Otter, T., King, S.M., and Witman, G.B. (1987) A two-step procedure for efficient electro transfer of both high-molecular weight (greater than 400,000) and low-molecular weight (less than 20,000) proteins. Anal Biochem. 162, 370–377.
    • (1987) Anal Biochem , vol.162 , pp. 370-377
    • Otter, T.1    King, S.M.2    Witman, G.B.3
  • 35
    • 0025013641 scopus 로고
    • Protein blotting: Ten years on
    • Review
    • Harper, D.R., Kit, M.L., and Kangro, H.O. (1990) Protein blotting: ten years on. J Virol Methods 30, 25–39. Review.
    • (1990) J Virol Methods , vol.30 , pp. 25-39
    • Harper, D.R.1    Kit, M.L.2    Kangro, H.O.3
  • 36
    • 0028457742 scopus 로고
    • Protein (west-ern) blotting
    • Egger, D., and Bienz, K. (1994) Protein (west-ern) blotting. Mol Biotechnol. 1, 289–305.
    • (1994) Mol Biotechnol , vol.1 , pp. 289-305
    • Egger, D.1    Bienz, K.2
  • 38
    • 0037374225 scopus 로고    scopus 로고
    • Protein blotting: A review
    • Review
    • Kurien, B.T., and Scofield, R.H. (2003) Protein blotting: a review. J Immunol Methods 274, 1–15. Review.
    • (2003) J Immunol Methods , vol.274 , pp. 1-15
    • Kurien, B.T.1    Scofield, R.H.2
  • 39
    • 85069302395 scopus 로고    scopus 로고
    • Blotting techniques
    • P.J. Worsfold, A. Townshend, and C.F. Poole (Eds.), Second edition. Elsevier, Oxford
    • Kurien, B.T., and Scofield, R.H. (2005) Blotting techniques. In: P.J. Worsfold, A. Townshend, and C.F. Poole (Eds.) Encyclopedia of analytical science, Second edition. Elsevier, Oxford, p 425.
    • (2005) Encyclopedia of Analytical Science , pp. 425
    • Kurien, B.T.1    Scofield, R.H.2
  • 40
    • 0020163542 scopus 로고
    • Protein transfer from isoelectric focusing gels: The native blot
    • Reinhart, M.P., and Malamud, D. (1982). Protein transfer from isoelectric focusing gels: the native blot. Anal Biochem. 123, 229–235.
    • (1982) Anal Biochem , vol.123 , pp. 229-235
    • Reinhart, M.P.1    Malamud, D.2
  • 41
    • 0024103310 scopus 로고
    • Blotting from PhastGel media after horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    • Jagersten, C., Edstrom, A., Olsson, B., and Jacobson, G. (1988) Blotting from PhastGel media after horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Electrophoresis 9, 662–665.
    • (1988) Electrophoresis , vol.9 , pp. 662-665
    • Jagersten, C.1    Edstrom, A.2    Olsson, B.3    Jacobson, G.4
  • 42
    • 0025268387 scopus 로고
    • Checkerboard immunoblotting (CBIB): An efficient, rapid, and sensitive method of assaying multiple antigen/antibody cross-reactivities
    • Kazemi, M., and Finkelstein R.A. (1990) Checkerboard immunoblotting (CBIB): an efficient, rapid, and sensitive method of assaying multiple antigen/antibody cross-reactivities. J Immunol Methods 128, 143–146.
    • (1990) J Immunol Methods , vol.128 , pp. 143-146
    • Kazemi, M.1    Finkelstein, R.A.2
  • 43
    • 0029041689 scopus 로고
    • Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix
    • Heukeshoven, J., and Dernick, R. (1995). Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix. Electrophoresis 16, 748–756.
    • (1995) Electrophoresis , vol.16 , pp. 748-756
    • Heukeshoven, J.1    Dernick, R.2
  • 44
    • 0031788025 scopus 로고    scopus 로고
    • Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support
    • Olsen, I., and Wiker, H.G. (1998) Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. J Immunol Methods 220, 77–84.
    • (1998) J Immunol Methods , vol.220 , pp. 77-84
    • Olsen, I.1    Wiker, H.G.2
  • 45
    • 0034938437 scopus 로고    scopus 로고
    • Simultaneous immunoblotting analysis with activity gel electrophoresis in a single polyacrylamide gel
    • Chen, H., and Chang, G.D. (2001) Simultaneous immunoblotting analysis with activity gel electrophoresis in a single polyacrylamide gel. Electrophoresis 22, 1894–1899.
    • (2001) Electrophoresis , vol.22 , pp. 1894-1899
    • Chen, H.1    Chang, G.D.2
  • 47
    • 0034069044 scopus 로고    scopus 로고
    • Association of neutropenia in systemic lupus erythematosus with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigen
    • Kurien, B.T., and Scofield, R.H. (2000) Association of neutropenia in systemic lupus erythematosus with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigen. Clin Exp Immunol. 120, 209–217.
    • (2000) Clin Exp Immunol , vol.120 , pp. 209-217
    • Kurien, B.T.1    Scofield, R.H.2
  • 48
    • 0035432908 scopus 로고    scopus 로고
    • Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane
    • Kurien, B.T., Matsumoto, H., and Scofield, R.H. (2001) Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane. Indian J Biochem Biophys. 38, 274–276.
    • (2001) Indian J Biochem Biophys , vol.38 , pp. 274-276
    • Kurien, B.T.1    Matsumoto, H.2    Scofield, R.H.3
  • 49
    • 0032526292 scopus 로고    scopus 로고
    • The detection of enzyme activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    • Review
    • Bischoff, K.M., Shi, L., and Kennelly, P.J. (1998) The detection of enzyme activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 260, 1–17. Review.
    • (1998) Anal Biochem , vol.260 , pp. 1-17
    • Bischoff, K.M.1    Shi, L.2    Kennelly, P.J.3
  • 51
    • 70350316065 scopus 로고    scopus 로고
    • A simpler and faster version of two-dimensional gel electrophoresis using vertical, mini SDS-PAGE apparatus
    • Dorri, Y., Kurien, B.T., and Scofield, R.H. (2009) A simpler and faster version of two-dimensional gel electrophoresis using vertical, mini SDS-PAGE apparatus. Iran J Chem Chem Eng. 28, 51–56.
    • (2009) Iran J Chem Chem Eng , vol.28 , pp. 51-56
    • Dorri, Y.1    Kurien, B.T.2    Scofield, R.H.3


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