Novel Multicolor Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species

Methods in Molecular Biology

Volumn 717, Issue , 2011, Pages 233-244

  Frisch, Jillian ^a   Houchins, J P ^a   Grahek, Michael ^a   Schoephoerster, Jordan ^a   Hagen, Jodi ^a   Sweet, Joseph ^a   Mendoza, Leopoldo ^b   Schwartz, David ^b   Kalyuzhny, Alexander E ^a  

^a R and D Systems Inc   (United States)

^b Aesthetics Inc   (United States)

Author keywords

Antibody conjugation, Biotinylation, Digoxigenin; Immunofluorescence, Immunohistochemistry; Phospho specific antibodies; Triple labeling

Indexed keywords

ANTIBODY; ANTIBODY CONJUGATE; DIGOXIGENIN; PHOSPHOSPECIFIC ANTIBODY;

ANIMAL; ARTICLE; BIOTINYLATION; CHEMISTRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; METHODOLOGY; RAT; SPRAGUE DAWLEY RAT;

ANIMALS; ANTIBODIES; ANTIBODIES, PHOSPHO-SPECIFIC; BIOTINYLATION; DIGOXIGENIN; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNOCONJUGATES; RATS; RATS, SPRAGUE-DAWLEY;

CAPRA HIRCUS; ORYCTOLAGUS CUNICULUS;

EID: 84855491213     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-024-9_13     Document Type: Chapter

References (11)

1. Kalyuzhny, A.E. (2009) The dark side of the immunohistochemical moon: industry. J. Histochem. Cytochem. 57, 1099–1101.
2. Wessendorf, M.W., Appel, N.M., Molitor, T.W., and Elde, R.P. (1990) A method for immunofluorescent demonstration of three coexisting neurotransmitters in rat brain and spinal cord, using the fluorophores fluores-cein, lissamine rhodamine, and 7-amino-4-methylcoumarin-3-acetic acid. J. Histochem. Cytochem. 38, 1859–1877.
3. Staines, W.A., Meister, B., Melander, T., Nagy, J.I., and Hökfelt, T. (1988) Three-color immunofluorescence histochemistry allowing triple labeling within a single section. J. Histochem. Cytochem. 36,145–151.
4. Wang, B.L., and Larsson, L.I. (1985) Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species. Histochemistry. 83, 47–56.
5. Van der Loos, C.M., Das, P.K., Van den Oord, J.J., and Houthoff, H.J. (1989) Multiple immu-noenzyme staining techniques. Use of fluores-ceinated, biotinylated and unlabelled monoclonal antibodies. J. Immunol. Methods. 117, 45–52.
6. Cavet, M.E., Lehoux, S., and Berk, B.C. (2003) 14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity. J. Biol. Chem. 278, 18376–1883.
7. Casar, B., Sanz-Moreno, V., Yazicioglu, M.N., Rodríguez, J., Berciano, M.T., Lafarga, M., et al., (2007) Mxi2 promotes stimulus-inde-pendent ERK nuclear translocation. EMBO J. 26, 635–646.
8. Takahashi, M., Buma, Y., Iwamoto, T., Inaguma, Y., Ikeda, H., and Hiai, H. (1988) Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene. 3, 571–578.
9. Brizzee, K.R., Ordy, J.M., and Kaack. B. (1974) Early appearance and regional differences in intraneuronal and extraneuronal lipofuscin accumulation with age in the brain of a nonhuman primate (Macaca mulatta). J. Gerontol. 29, 366–381.
10. Dowson, J.H., Armstrong, D., Koppang, N., Lake, B.D., and Jolly, R.D. (1982) Autofluorescence emission spectra of neuronal lipopigment in animal and human ceroidoses (ceroid-lipofuscinoses). Acta Neuropathol. 58, 152–156.
11. Schnell, S.A., Staines, W.A., and Wessendorf, M.W. (1999) Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J. Histochem. Cytochem. 47, 719–730.