A quantitative comet infection assay for influenza virus

Journal of Virological Methods

Volumn 179, Issue 2, 2012, Pages 351-358

  Lindsay, Stephen M ^a   Timm, Andrea ^a   Yin, John ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Antiviral assay; Comet; Evaporation; Image analysis; Influenza; Plaque

Indexed keywords

ALPHA2 6 SIALIC ACID VIRUS RECEPTOR; INFLUENZA VIRUS RECEPTOR; RIBAVIRIN; UNCLASSIFIED DRUG; VIRUS RECEPTOR;

ANIMAL CELL; ANTIVIRAL SUSCEPTIBILITY; ARTICLE; CELL SIZE; CELL STRAIN; CELL SURFACE; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG SCREENING; EVAPORATION; FLUID FLOW; HOST CELL; HUMIDITY; INFLUENZA VIRUS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIBODIES, VIRAL; ANTIVIRAL AGENTS; CELL LINE; DOGS; HUMIDITY; ORTHOMYXOVIRIDAE; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; VIRAL PLAQUE ASSAY;

ORTHOMYXOVIRIDAE;

EID: 84855476144     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.11.022     Document Type: Article

References (20)

1. Anekal S.G., Zhu Y., Graham M.D., Yin J. Dynamics of virus spread in the presence of fluid flow. Integr. Biol. 2009, 1:664-671.
2. Barefoot B., Thornburg N.J., Barouch D.H., Yu J., Sample C., Johnston R.E., Liao H.X., Kepler T.B., Haynes B.F., Ramsburg E. Comparison of multiple vaccine vectors in a single heterologous prime-boost trial. Vaccine 2008, 26:6108-6118.
3. Brown M.B., Forsythe A.B. Robust tests for the equality of variances. J. Am. Stat. Assoc. 1974, 69:364-367.
4. Dulbecco R. Production of plaques in monolayer tissue cultures by single particles of an animal virus. Proc. Natl. Acad. Sci. U. S. A. 1952, 38:747-752.
5. Fenteany G., Janmey P.A., Stossel T.P. Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets. Curr. Biol. 2000, 10:831-838.
6. Gambaryan A.S., Matrosovich M.N., Bender C.A., Kilbourne E.D. Differences in the biological phenotype of low-yielding (L) and high-yielding (H) variants of swine influenza virus A/NJ/11/76 are associated with their different receptor-binding activity. Virology 1998, 247:223-231.
7. Graci J.D., Cameron C.E. Mechanisms of action of ribavirin against distinct viruses. Rev. Med. Virol. 2006, 16:37-48.
8. Hatakeyama S., Sakai-Tagawa Y., Kiso M., Goto H., Kawakami C., Mitamura K., Sugaya N., Suzuki Y., Kawaoka Y. Enhanced expression of an alpha2,6-linked sialic acid on MDCK cells improves isolation of human influenza viruses and evaluation of their sensitivity to a neuraminidase inhibitor. J. Clin. Microbiol. 2005, 43:4139-4146.
9. Law M., Hollinshead R., Smith G.L. Antibody-sensitive and antibody-resistant cell-to-cell spread by vaccinia virus: role of the A33R protein in antibody-resistant spread. J. Gen. Virol. 2002, 83:209-222.
10. Li S., Schulman J., Itamura S., Palese P. Glycosylation of neuraminidase determines the neurovirulence of influenza A/WSN/33 virus. J. Virol. 1993, 67:6667-6673.
11. Matrosovich M., Matrosovich T., Carr J., Roberts N.A., Klenk H.D. Overexpression of the alpha-2,6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors. J. Virol. 2003, 77:8418-8425.
12. Matsubayashi Y., Ebisuya M., Honjoh S., Nishida E. ERK activation propagates in epithelial cell sheets and regulates their migration during wound healing. Curr. Biol. 2004, 14:731-735.
13. Nikolic D.L., Boettiger A.N., Bar-Sagi D., Carbeck J.D., Shvartsman S.Y. Role of boundary conditions in an experimental model of epithelial wound healing. Am. J. Physiol. Cell Physiol. 2006, 291:C68-C75.
14. Olson V.A., Karem K.L., Smith S.K., Hughes C.M., Damon I.K. Smallpox virus plaque phenotypes: genetic, geographical and case fatality relationships. J. Gen. Virol. 2009, 90:792-798.
15. Poujade M., Grasland-Mongrain E., Hertzog A., Jouanneau J., Chavrier P., Ladoux B., Buguin A., Silberzan P. Collective migration of an epithelial monolayer in response to a model wound. Proc. Natl. Acad. Sci. U. S. A. 2007, 104:15988-15993.
16. Rasband, W.S., 1997-2011. ImageJ. U.S. National Institutes of Health, Bethesda, MD, USA. http://imagej.nih.gov/ij/.
17. San Biagio P.L., Bulone D., Emanuele A., Palma-Vittorelli M.B., Palma M.U. Spontaneous symmetry-breaking pathways: time-resolved study of agarose gelation. Food Hydrocoll. 1996, 10:91-97.
18. Smith S.K., Olson V.A., Karem K.L., Jordan R., Hruby D.E., Damon I.K. In vitro efficacy of ST246 against smallpox and monkeypox. Antimicrob. Agents Chemother. 2009, 53:1007-1012.
19. Wilck M.B., Seaman M.S., Baden L.R., Walsh S.R., Grandpre L.E., Devoy C., Giri A., Kleinjan J.A., Noble L.C., Stevenson K.E., Kim H.T., Dolin R. Safety and immunogenicity of modified vaccinia ankara (ACAM3000): effect of dose and route of administration. J. Infect. Dis. 2010, 201:1361-1370.
20. Zhu Y., Yin J. A quantitative comet assay: imaging and analysis of virus plaques formed with a liquid overlay. J. Virol. Methods 2007, 139:100-102.