Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus

Journal of Bacteriology

Volumn 193, Issue 23, 2011, Pages 6498-6504

  Bridger, Stephanie L ^a   Clarkson, Sonya M ^a   Stirrett, Karen ^a,b   Debarry, Megan B ^a   Lipscomb, Gina L ^a   Schut, Gerrit J ^a   Westpheling, Janet ^a   Scott, Robert A ^a   Adams, Michael W W ^a  

^a University of Georgia ^*  (United States)

^b Southeastern Community College   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEN SULFIDE; MALTOSE; OXIDOREDUCTASE; URACIL; ARCHAEAL PROTEIN; MEMBRANE PROTEIN; SIPA PROTEIN, PYROCOCCUS FURIOSUS; SULFUR;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; DETOXIFICATION; ENERGY CONSERVATION; GENE DELETION; GROWTH CURVE; HOMOLOGOUS RECOMBINATION; IRON METABOLISM; NONHUMAN; PRIORITY JOURNAL; PYROCOCCUS FURIOSUS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION;

PYROCOCCUS FURIOSUS;

ARCHAEAL PROTEINS; GENE EXPRESSION REGULATION, ARCHAEAL; MEMBRANE PROTEINS; OXIDATION-REDUCTION; OXIDOREDUCTASES; PYROCOCCUS FURIOSUS; SEQUENCE DELETION; SULFUR;

EID: 84855412663     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.05445-11     Document Type: Article

References (27)

1. Adams, M. W., et al. 2001. Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus. J. Bacteriol. 183:716-724.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Chen, J. S., and L. E. Mortenson. 1977. Inhibition of methylene blue formation during determination of the acid-labile sulfide of iron-sulfur protein samples containing dithionite. Anal. Biochem. 79:157-165.
4. Clarkson, S. M., E. C. Newcomer, E. G. Young, and M. W. Adams. 2010. The elemental sulfur-responsive protein (SipA) from the hyperthermophilic archaeon Pyrococcus furiosus is regulated by sulfide in an iron-dependent manner. J. Bacteriol. 192:5841-5843.
5. Fiala, G. a, K. O. S. 1986. Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C. Arch. Microbiol. 145:56-61.
6. Hagen, W. R., et al. 2000. Novel structure and redox chemistry of the prosthetic groups of the iron-sulfur flavoprotein sulfide dehydrogenase from Pyrococcus furiosus; evidence for a [2Fe-2S] cluster with Asp(Cys)3 ligands. J. Biol. Inorg. Chem. 5:527-534.
7. Hatton, B., and D. Rickard. 2008. Nucleic acids bind to nanoparticulate iron (II) monosulphide in aqueous solutions. Orig. Life Evol. Biosph. 38:257-270.
8. Jang, S., and J. A. Imlay. 2007. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. J. Biol. Chem. 282: 929-937.
9. Kanai, T., et al. 2011. Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis. J. Bacteriol. 193:3109-3116.
10. Kleinjan, W. E., A. de Keizer, and A. J. Janssen. 2005. Kinetics of the chemical oxidation of polysulfide anions in aqueous solution. Water Res. 39:4093-4100.
11. Kleinjan, W. E., A. de Keizer, and J. H. Janssen. 2003. Biologically produced sulfur. Top. Curr. Chem. 230:167-188.
12. Lipscomb, G. L., et al. 2009. SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus. Mol. Microbiol. 71:332-349.
13. Lipscomb, G. L., et al. 2011. Natural competence in the hyperthermophilic archaeon Pyrococcus furiosus facilitates genetic manipulation: construction of multiple markerless deletions of genes encoding the two cytoplasmic hydrogenases. Appl. Environ. Microbiol. 77:2232-2238.
14. Liu, Y., M. Sieprawska-Lupa, W. B. Whitman, and R. H. White. 2010. Cysteine is not the sulfur source for iron-sulfur cluster and methionine biosynthesis in the methanogenic archaeon Methanococcus maripaludis. J. Biol. Chem. 285:31923-31929.
15. Ma, K., and M. W. Adams. 2001. Ferredoxin:NADP oxidoreductase from Pyrococcus furiosus. Methods Enzymol. 334:40-45.
16. Ma, K., and M. W. Adams. 1994. Sulfide dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus: a new multifunctional enzyme involved in the reduction of elemental sulfur. J. Bacteriol. 176:6509-6517.
17. Ma, K., R. N. Schicho, R. M. Kelly, and M. W. Adams. 1993. Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor. Proc. Natl. Acad. Sci. U. S. A. 90:5341-5344.
18. Miller, R. E., and E. R. Stadtman. 1972. Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. J. Biol. Chem. 247:7407-7419.
19. Santangelo, T. J., L. Cubonova, and J. N. Reeve. 2011. Deletion of alternative pathways for reductant recycling in Thermococcus kodakarensis increases hydrogen production. Mol. Microbiol. 81:897-911.
20. Sapra, R., K. Bagramyan, and M. W. Adams. 2003. A simple energy-conserving system: proton reduction coupled to proton translocation. Proc. Natl. Acad. Sci. U. S. A. 100:7545-7550.
21. Schut, G. J., S. D. Brehm, S. Datta, and M. W. Adams. 2003. Whole-genome DNA microarray analysis of a hyperthermophile and an archaeon: Pyrococcus furiosus grown on carbohydrates or peptides. J. Bacteriol. 185:3935-3947.
22. Schut, G. J., S. L. Bridger, and M. W. Adams. 2007. Insights into the metabolism of elemental sulfur by the hyperthermophilic archaeon Pyrococcus furiosus: characterization of a coenzyme A-dependent NAD(P)H sulfur oxidoreductase. J. Bacteriol. 189:4431-4441.
23. Schut, G. J., J. Zhou, and M. W. Adams. 2001. DNA microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus: evidence for a new type of sulfur-reducing enzyme complex. J. Bacteriol. 183:7027-7036.
24. Silva, P. J., et al. 2000. Enzymes of hydrogen metabolism in Pyrococcus furiosus. Eur. J. Biochem. 267:6541-6551.
25. Strand, K. R., et al. 2010. Oxidative stress protection and the repair response to hydrogen peroxide in the hyperthermophilic archaeon Pyrococcus furiosus and in related species. Arch. Microbiol. 192:447-459.
26. Williams, E., T. M. Lowe, J. Savas, and J. DiRuggiero. 2007. Microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus exposed to gamma irradiation. Extremophiles 11:19-29.
27. Yang, H., et al. 2010. SurR regulates hydrogen production in Pyrococcus furiosus by a sulfur-dependent redox switch. Mol. Microbiol. 77:1111-1122.