1H, 13C and 15N resonance assignment of a 114-residue fragment of Engrailed 2 homeoprotein, a partially disordered protein

Biomolecular NMR Assignments

Volumn 5, Issue 2, 2011, Pages 229-231

  Augustyniak, Rafal ^a,b,c   Balayssac, Stéphane ^d   Ferrage, Fabien ^a,b,c   Bodenhausen, Geoffrey ^a,b,c,e   Lequin, Olivier ^a,b,c  

^a SORBONNE UNIVERSITÉ   (France)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c CNRS   (France)

^d UPS)   (France)

^e INSTITUTE OF CHEMICAL SCIENCES AND ENGINEERING   (Switzerland)

Author keywords

Disordered proteins; Engrailed 2; Homeodomain; NMR; Transcription factor

Indexed keywords

ENGRAILED 2 PROTEIN; HOMEODOMAIN PROTEIN; ISOTOPE; NERVE PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; CHICKEN; NUCLEAR MAGNETIC RESONANCE; PROTEIN CONFORMATION;

ANIMALS; CHICKENS; HOMEODOMAIN PROTEINS; ISOTOPES; NERVE TISSUE PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 84855391140     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-011-9306-5     Document Type: Article

References (16)

1. Alberi L, Sgado P, Simon HH (2004) Engrailed genes are cellautonomously required to prevent apoptosis in mesencephalic dopaminergic neurons. Development 131: 3229-3236
2. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6: 277-293
3. Foucher I, Montesinos ML, Volovitch M, Prochiantz A, Trembleau A (2003) Joint regulation of the MAP1B promoter by HNF3beta/ Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors. Development 130: 1867-1876
4. Fraenkel E, Rould MA, Chambers KA, Pabo CO (1998) Engrailed homeodomain-DNA complex at 2.2 A ° resolution: a detailed view of the interface and comparison with other engrailed structures. J Mol Biol 284: 351-361
5. Gehring WJ (1987) Homeo boxes in the study of development. Science 236: 1245-1252
6. Gehring WJ, Affolter M, Buerglin T (1994) Homeodomain proteins. Ann Rev Biochem 63: 487-526
7. Joyner AL (1996) Engrailed, Wnt and Pax genes regulate midbrainhindbrain development. Trends Genet 12: 15-20
8. Kazimierczuk K, Zawadzka A, Kozminski W (2009) Narrow peaks and high dimensionalities: exploiting the advantages of random sampling. J Magn Reson 197: 210-228
9. Maizel A, Tassetto M, Filhol O, Cochet C, Prochiantz A, Joliot A (2002) Engrailed homeoprotein secretion is a regulated process. Development 129: 3545-3553
10. Marley J, Lu M, Bracken C (2001) A method for efficient isotopic labelling of recombinant proteins. J Biomol NMR 20: 71-75
11. McGinnis W, Levine MS, Hafen E, Kuroiwa A, Gehring WJ (1984) A conserved DNA sequence in homeotic genes of the Drosophila Antennapedia and bithorax complex. Nature 308: 428-433
12. Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C (1999) Structure of the HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and the fourth homeodomain helix in complex formation. Cell 96: 587-597
13. Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Magn Reson Spectrosc 34: 93-158
14. Simon HH, Saueressig H, Wurst W, Goulding MD, O'Leary DD (2001) Fate of midbrain dopaminergic neurons controlled by the engrailed genes. J Neurosci 21: 3126-3134
15. Sonnier L, Le Pen G, Hartmann A, Bizot JC, Trovero F, Krebs MO, Prochiantz A (2007) Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1. J Neurosci 27: 1063-1071
16. Vranken WF, Boucher W, Stevens TJ et al (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59: 687-696