Feasibility of flowcytometric quantitation of immune effector cell subsets in the sentinel lymph node of the breast after cryopreservation

Journal of Immunological Methods

Volumn 375, Issue 1-2, 2012, Pages 189-195

  van Pul, Kim M ^a   Vuylsteke, Ronald J C L M ^b   Bril, Herman ^a   Stockmann, Hein B A C ^a   de Gruijl, Tanja D ^c  

^a Department of Orthopaedic Surgery   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cryopreservation; Dendritic cells; Immunophenotype; Quantitation; Regulatory T cells; Sentinel lymph node

Indexed keywords

CD14 ANTIGEN; CD83 ANTIGEN; CD86 ANTIGEN; GLYCOPROTEIN P 15095; T6 ANTIGEN; TRANSCRIPTION FACTOR FOXP3;

ANTIGEN EXPRESSION; ARTICLE; BREAST CANCER; CANCER PATIENT; CD4 CD8 RATIO; CD4+ CD25+ T LYMPHOCYTE; CELL COUNT; CLINICAL ARTICLE; CRYOPRESERVATION; DENDRITIC CELL; EFFECTOR CELL; FEASIBILITY STUDY; FLOW CYTOMETRY; FREEZE THAWING; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; INTERMETHOD COMPARISON; PILOT STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE STUDY; SENTINEL LYMPH NODE; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION;

AGED; BREAST NEOPLASMS; CD4-CD8 RATIO; CD8-POSITIVE T-LYMPHOCYTES; CRYOPRESERVATION; DENDRITIC CELLS; FEMALE; FLOW CYTOMETRY; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; LYMPH NODES; MIDDLE AGED; SENTINEL LYMPH NODE BIOPSY; T-LYMPHOCYTE SUBSETS;

EID: 84855348255     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.10.011     Document Type: Article

References (26)

1. Badwe R.A., Thorat M.A., Parmar V.V. Sentinel-node biopsy in breast cancer. New Engl J. Med. 2003, 349:1968.
2. Bembenek A., Li J., Loddenkemper C., Kemmner W., Stein H., Wernecke K.D., Schlag P.M. Presence of mature DC-Lamp+ dendritic cells in sentinel and non-sentinel nodes of breast cancer patients. Eur. J. Surg. Oncol. 2008, 34:514.
3. Costantini A., Mancini S., Giuliodoro S., Butini L., Regnery C.M., Silvestri G., Montroni M. Effects of cryopreservation on lymphocyte immunophenotype and function. J. Immunol. Methods 2003, 278:145.
4. Dunn G.P., Old L.J., Schreiber R.D. The immunobiology of cancer immunosurveillance and immunoediting. Immunity 2004, 21:137.
5. Elkord E. Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. J. Immunol. Methods 2009, 347:87.
6. Elliott B., Cook M.G., John R.J., Powell B.W., Pandha H., Dalgleish A.G. Successful live cell harvest from bisected sentinel lymph nodes: research report. J. Immunol. Methods 2004, 291:71.
7. Feuerstein B., Berger T.G., Maczek C., Röder C., Schreiner D., Hirsch U., Haendle I., Leisang W., Glaser A., Kuss O., Diepgen T.L., Schuler G., Schuler-Thurner B. A method for the production of cryopreserved aliquots of antigen-preloaded, mature dendritic cells ready for clinical use. J. Immunol. Methods 2000, 245:15.
8. Hori S., Heike Y., Takei M., Maruyama M., Inoue Y., Lee J., Kim H., Harada Y., Kawai H., Shimosaka A., Kami M., Tanosaki R., Wakasugi H., Saito S., Takaue Y., Kakizoe T. Freeze-thawing procedures have no influence on the phenotypic and functional development of dendritic cells generated from peripheral blood CD14+ monocytes. J. Immunother. 2004, 27:27.
9. Huang R.R., Wen D.R., Guo J., Giuliano A.E., Nguyen M., Offodile R., Stern S., Turner R., Cochran A.J. Selective modulation of paracortical dendritic cells and T-lymphocytes in breast cancer sentinel lymph nodes. Breast J. 2000, 6:225.
10. Kim T., Giuliano A.E., Lyman G.H. Lymphatic mapping and sentinel lymph node biopsy in early-stage breast carcinoma: a meta-analysis. Cancer 2006, 106:4.
11. Kohrt H.E., Nouri N., Nowels K., Johnson D., Holmes S., Lee P.P. Profile of immune cells in axillary lymph nodes predicts disease-free survival in breast cancer. PLoS Med. 2005, 2:904.
12. Krag D., Weaver D., Ashikaga T., et al. The sentinel node in breast cancer - a multicenter validation study. New Engl J. Med. 1998, 339:941.
13. Lewalle P., Rouas R., Lehmann F., Martiat P. Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli. J. Immunol. Methods 2000, 240:69.
14. Makino M., Baba M. Cryopreservation method of human peripheral blood mononuclear cells for efficient production of dendritic cells. Scand. J. Immunol. 1997, 45:618.
15. Mansfield A.S., Heikkila P.S., Vaara A.T., von Smitten K.A.J., Vakkila J.M., Leidenius M.H.K. Simultaneous Foxp3 and IDO expression is associated with sentinel lymph node metastases in breast cancer. BMC Cancer 2009, 9:231.
16. Matsuura K., Yamagushi Y., Ueno H., Osaki A., Arihiro K., Toge T. Maturation of dendritic cells and T-cell responses in sentinel lymph nodes from patients with breast carcinoma. Cancer 2006, 106:1227.
17. Molenkamp B.G., van Leeuwen P.A.M., Meijer S., Sluijter B.J.R., Wijnands P.G.J.T.B., Baars A., van den Eertwegh A.J.M., Scheper R.J., de Gruijl T.D. Intradermal CpG-B activates both plasmacytoid and myeloid dendritic cells in the sentinel lymph node of melanoma patients. Clin. Cancer Res. 2007, 13:2961.
18. Nakamura R., Sakakibara M., Nagashima T., Sangai T., Arai M., Fujimori T., Takano S., Shida T., Nakatani Y., Miyazaki M. Accumulation of regulatory T cells in sentinel lymph nodes is a prognostic predictor in patients with node-negative breast cancer. Eur. J. Cancer 2009, 45:2123.
19. Ostrand-Rosenberg S., Sinha P. Myeloid-derived suppressor cells: linking inflammation and cancer. J. Immunol. 2009, 182:449.
20. Poindexter N.J., Sahin A., Hunt K.K., Grimm E.A. Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer. Breast Cancer Res. 2004, 6:408.
21. Reimann K.A., Chernoff M., Wilkening C.L., Nickerson C.E., Landay A.L. Preservation of lymphocyte immunophenotype and proliferative responses in cryopreserved peripheral blood mononuclear cells from human immunodeficiency virus type 1-infected donors: implications for multicenter clinical trials. Clin. Diagn. Lab. Immunol. 2000, 7:352.
22. Taylor M.J., London N.J.M., Thirdborough S.M., Lake S.P., James R.F.L. The cryobiology of rat and human dendritic cells: preservation and destruction of membrane integrity by freezing. Cryobiology 1990, 27:269.
23. Torrenga H., Meijer S., Fabry H., van der Sijp J. Sentinel node biopsy in breast cancer patients: triple technique as a routine procedure. Ann. Surg. Oncol. 2004, 11:231.
24. van Hemelen D., Oude Elberink J.N.G., Heimweg J., van Oosterhout A.J.M., Nawijn M.C. Cryopreservation does not alter the frequency of regulatory T cells in peripheral blood mononuclear cells. J. Immunol. Methods 2010, 353:138.
25. Vuylsteke R.J.C.L.M., van Leeuwen P.A.M., Meijer S., Wijnands P.G.J.T.B., Statius Muller M.G., Busch D.H., Scheper R.J., de Gruijl T.D. Sampling tumor-draining lymph nodes for phenotypic and functional analysis of dendritic cells and T cells. Am. J. Pathol. 2002, 161:19.
26. Westermann J., Körner I.J., Kopp J., Kurz S., Zenke M., Dörken B., Pezzutto A. Cryopreservation of mature monocyte-derived human dendritic cells for vaccination: influence on phenotype and functional properties. Cancer Immunol. Immunother. 2003, 52:194.