A conserved glutamate residue, Glu-257, is important for substrate binding and transport by the Escherichia coli mannitol permease

Journal of Bacteriology

Volumn 179, Issue 4, 1997, Pages 1135-1142

  Saraceni Richards, Cynthia A ^a,b   Jacobson, Gary R ^a  

^a BOSTON UNIVERSITY   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MANNITOL; PERMEASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CELL MEMBRANE TRANSPORT; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0031046661     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.4.1135-1142.1997     Document Type: Article

References (44)

1. Begley, G. S., D. E. Hansen, G. R. Jacobson, and J. R. Knowles. 1982. Stereo-chemical course of the reactions catalyzed by the bacterial phosphocnolpyruvate:glucose phosphotransferase system. Biochemistry 21:5552-5556.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Briggs, C. E., S. S. Khandekar, and G. R. Jacobson. 1992. Structure/function relationships in the Escherichia coli mannitol permease: identification of regions important for membrane insertion, substrate binding, and oligomorization. Res. Microbiol. 143:139-149.
4. Chen, Z. J., L. Parent, and T. Maniatis. 1996. Site-specific phosphorylation of IκBα by a novel ubiquitination-dependent protein kinase activity. Cell 84:853-862.
5. Deng, W. P., and J. A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
6. Elferink, M. G. L., A. J. M. Driessen, and G. T. Robillard. 1990. Functional reconstitution of the purified phosphoenolpyruvate-dependent mannitol-specific transport system of Escherichia coli in phospholipid vesicles: coupling between transport and phosphorylation. J. Bacteriol. 172:7119-7125.
7. Grisafi, P. L., A. Scholle, J. Sugiyama, C. Briggs, G. R. Jacobson, and J. W. Lengeler. 1989. Deletion mutants of the Escherichia coli K-12 mannitol permease: dissection of transport, phosphorylation, phospho-exchange, and mannitol-binding activities. J. Bacteriol. 171:2719-2727.
8. 7a. Heuel, H., and J. Lengeler. Personal communication.
9. Jacobson, G. R., C. A. Lee, and M. H. Saier, Jr. 1979. Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system. J. Biol. Chem. 254:249-252.
10. Jacobson, G. R. 1992. Interrelationships between protein phosphorylation and oligomerization in transport and chemotaxis via the Escherichia coli mannitol phosphotransferase system. Res. Microhiol. 143:113-116.
11. Jacobson, G. R., and C. Saraceni-Richards. 1993. The Escherichia coli mannitol permease as a model for transport via the bacterial phosphotransferase system. J. Bioenerget. Biomembr. 25:621-626.
12. Khandekar, S. S., and G. R. Jacobson. 1989. Evidence for two distinct conformations of the Escherichia coli mannitol permease that are important for its transport and phosphorylation functions. J. Cell. Biochem. 39:207-216.
13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
14. Lee, C. A., and M. H. Saier, Jr. 1983. Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. Nucleotide sequence of the permease gene. J. Biol. Chem. 258:10761-10767.
15. Lengeler, J. W. 1990. Molecular analysis of the enzyme II-complexes of the bacterial phosphotransferase system (PTS) as carbohydrate transport systems. Biochim. Biophys. Acta 1018:155-159.
16. Lengeler, J. W., F. Titgemeyer, A. P. Vogler, and B. M. Wöhrl. 1990. Structure and homnlogies of carbohydrate:phosphotransferase system (PTS) proteins. Philos. Trans. R. Soc. Lond. B 326:489-504.
17. Lengeler, J. W., and A. P. Vogler. 1989. Molecular mechanisms of bacterial chemotaxis towards PTS-carbohydrates. FEMS Microbiol. Rev. 63:81-92.
18. Mtl of Escherichia coli constitutes a mannitol translocation unit. Biochemistry 29:10659-10663.
19. Mtl of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. Biochemistry 30:6716-6721.
20. Mtl of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. Biochemistry 30:6721-6726.
21. Mtl of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. Biochemistry 31:5514-5521.
22. Manayan, R., G. Tenn, H. B. Yee, J. D. Desai, M. Yamada, and M. H. Saier, Jr. 1988. Genetic analysis of the mannitol permease of Escherichia coli: isolation and characterization of a transport-deficient mutant which retains phosphorylating activity. J. Baeteriol. 170:1290-1296.
23. Mandel, M., and A. Higa. 1970. Calcium dependent bacteriophage DNA infection. J. Mol. Biol. 53:159-162.
24. Meadow, N. D., D. K. Fox, and S. Roseman. 1990. The bacterial phosphoenolpyruvate:glycose phosphotransferase system. Annu. Rev. Biochem. 59:497-542.
25. Mtl. Biochemistry 27:5835-5839.
26. Pas, H. H., R. H. ten Hoeve-Duurkens, and G. T. Robillard. 1988. Bacterial phosphoenolpyruvate-dependent phosphotransferase system: mannitol-specific EII contains two phosphoryl binding sites per monomer and one high-affinity mannitol binding site per dimer. Biochemistry 27:5520-5525.
27. Mtl. J. Biol. Chem. 266:6690-6692.
28. Postma, P. W., J. W. Lengeler, and G. R. Jacobson. 1993. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria, Microbiol. Rev. 57:543-594.
29. Glc of Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. J. Bacteriol. 174:2843-2850.
30. Saier, M. H., Jr., and J. Reizer. 1992. Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J. Baeteriol. 174:1433-1438.
31. Saier, M. H., Jr., A. Reizer, G. M. Pao, L.-F. Wu, J. Reizer, and A. H. Romano. 1992. Evolution of the bacterial phosphoenolpyruvate:sugar phosphotransferase system. Section II. Molecular considerations, p. 171-204. In R. P. Mortlock (ed.), Evolution of metabolic function. CRC Press, Inc., Boca Raton. Fla.
32. Saier, M. H., Jr., D. R. Simoni, and S. Roseman. 1976. Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate:sugar phosphotransferase system. J. Biol. Chem. 251:6584-6597.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
34. Sayers, J. R., and F. Eckstein. 1989. Site-directed mutagenesis, based on the phosphorothioate approach, p. 279-296. In T. S. Creighton (ed.), Protein function. IRL Press, New York, N.Y.
35. Scatchard, G. 1949. The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sc. 51:660-672.
36. Stephan, M. M., and G. R. Jacobson. 1986. Membrane disposition of the Escherichia coli mannitol permease: identification of membrane-bound and cytoplasmic domains. Biochemistry 25:4046-4051.
37. Stephan, M. M., and G. R. Jacobson. 1986. Subunit interactions of the Escherichia coli mannitol permease: correlations with enzyme activities. Biochemistry 25:8230-8234.
38. Sugiyama, J. E., S. Mahmoodian, and G. R. Jacobson. 1991. Membrane topology analysis of the Escherichia coli mannitol permease by using a nested deletion method to create mtlA-phoA fusions. Proc. Natl. Acad. Sci. USA 88:9603-9607.
39. Mtl. Biochemistry 30:1774-1779.
40. van Weeghel, R. P., G. H. Meyer, H. H. Pas, W. Keck, and G. T. Robillard. 1991. Cytoplasmic phosphorylating domain of the mannitol-specific transport proteins of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli: overexpression, purification and functional complementation with the mannitol binding domain. Biochemistry 30:9478-9485.
41. van Weeghel, R. P., Y. Y. van der Hoek, H. H. Pas, M. Elferink, W. Keck, and G. T. Robillard. 1991. Details of mannitol transport in Escherichia coli elucidated by site-specific mutagenesis and complementation of phosphorylation site mutants of the phosphoenolpyruvate-dependent mannitol-specific phosphotransferase system. Biochemistry 30:1768-1773.
42. Weng, Q.-P., J. Elder, and G. R. Jacobson. 1992. Site-specific mutagenesis of residues in the Escherichia coli mannitol permease that have been suggested to be important for its phosphorylation and chemoreception functions. J. Biol. Chem. 267:19529-19535.
43. Weng, Q.-P., and G. R. Jacobson. 1993. Role of a conserved histidine residue, His-195, in the activities of the Escherichia coli mannitol permease. Biochemistry 32:11211-11216.
44. White, D. W., and G. R. Jacobson. 1990. Molecular cloning of the C-terminal domain of Escherichia coli D-mannitol permease: expression, phosphorylation, and complementation with C-terminal permease deletion mutants. J. Bacteriol. 172:1509-1515.