The presence of three repeats in the 5′ UTR region of thymidylate synthase (TS) is associated with increased TS mRNA expression in cultured human cancer cell lines in vitro

Oncology Reports

Volumn 27, Issue 1, 2012, Pages 246-249

  Hammad, Hiba ^a   Sarkar, Malancha ^a   Gupta, Nikhil ^a   Ardalan, Bach ^a   Subbarayan, Pochi R ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

3 UTR insertion; Genotypic analysis; Real time PCR; Thymidylate synthase mRNA level; Translation regulation; TS enhancer region

Indexed keywords

MESSENGER RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ALLELE; ARTICLE; CANCER CELL CULTURE; COLORECTAL CANCER; CONTROLLED STUDY; FEMALE; GENE; GENE EXPRESSION; GENETIC POLYMORPHISM; GENOTYPE; HOMOZYGOSITY; HUMAN; HUMAN CELL; IN VITRO STUDY; LUNG CANCER; OSTEOSARCOMA CELL; PANCREAS CANCER; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TANDEM REPEAT; THYMIDYLATE SYNTHASE GENE; UTERINE CERVIX CANCER;

5' UNTRANSLATED REGIONS; BASE SEQUENCE; CELL LINE, TUMOR; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENOTYPE; HUMANS; MOLECULAR SEQUENCE DATA; NEOPLASMS; POLYMORPHISM, GENETIC; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER; THYMIDYLATE SYNTHASE;

EID: 84855269908     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2011.1469     Document Type: Article

References (19)

1. Heidelberger C, Danenberg PV and Moran RG: Fluorinated pyrimidines and their nucleosides. Adv Enzymol Relat Areas Mol Biol 54: 58-119, 1983.
2. Peters GJ, Backus HH, Freemantle S, et al: Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim Biophys Acta 1587: 194-205, 2002. (Pubitemid 34655788)
3. Danenberg PV: Thymidylate synthetase - a target enzyme in cancer chemotherapy. Biochim Biophys Acta 473: 73-92, 1977.
4. Kaneda S, Takeishi K, Ayusawa D, Shimizu K, Seno T and Altman S: Role in translation of a triple tandemly repeated sequence in the 5'-untranslated region of human thymidylate synthase mRNA. Nucleic Acids Res 15: 1259-1270, 1987. (Pubitemid 17012904)
5. Mandola MV, Stoehlmacher J, Zhang W, et al: A 6-bp polymorphism in the thymidylate synthase gene causes message instability and is associated with decreased intratumoral TS mRNA levels. Pharmacogenetics 14: 319-327, 2004. (Pubitemid 38638919)
6. Marsh S, Ameyaw MM, Githang'a J, Indalo A, Ofori-Adjei D and McLeod HL: Novel thymidylate synthase enhancer region alleles in African populations. Hum Mutat 16: 528, 2000.
7. Pullarkat ST, Stoehlmacher J, Ghaderi V, et al: Thymidylate synthase gene polymorphism determines response and toxicity of 5-FU chemotherapy. Pharmacogenomics J 1: 65-70, 2001. (Pubitemid 33757985)
8. Johnston PG, Lenz HJ, Leichman CG, Danenberg KD, Allegra CJ, Danenberg PV and Leichman L: Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors. Cancer Res 55: 1407-1412, 1995.
9. Mandola MV, Stoehlmacher J, Muller-Weeks S, Cesarone G, Yu MC, Lenz HJ and Ladner RD: A novel single nucleotide polymorphism within the 5′ tandem repeat polymorphism of the thymidylate synthase gene abolishes USF-1 binding and alters transcriptional activity. Cancer Res 63: 2898-2904, 2003. (Pubitemid 36667162)
10. Gusella M and Padrini R: G>C SNP of thymidylate synthase with respect to colorectal cancer. Pharmacogenomics 8: 985-996, 2007. (Pubitemid 47423688)
11. Ulrich CM, Bigler J, Velicer CM, Greene EA, Farin FM and Potter JD: Searching expressed sequence tag databases: discovery and confirmation of a common polymorphism in the thymidylate synthase gene. Cancer Epidemiol Biomarkers Prev 9: 1381-1385, 2000. (Pubitemid 32044809)
12. Grzybowska EA, Wilczynska A and Siedlecki JA: Regulatory Functions of 3'UTRs. Biochem Biophys Res Commun 288: 291-295, 2001.
13. Sambrook J and Russell DW: Molecular Cloning: A Laboratory Manual. 3rd edition. Cold Spring Harbor Laboratory Press, New York, 2001.
14. Villafranca E, Okruzhnov Y, Dominguez MA, et al: Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer. J Clin Oncol 19: 1779-1786, 2001.
15. Subbarayan PR, Sarkar M and Ardalan B: Isolation of genomic DNA from human whole blood. Biotechniques 33: 1231-1234, 2002. (Pubitemid 36008176)
16. Subbarayan PR, Lee K and Ardalan B: Arsenic trioxide suppresses thymidylate synthase in 5-FU-resistant colorectal cancer cell line HT29 in vitro re-sensitizing cells to 5-FU. Anticancer Res 30: 1157-1162, 2010.
17. Kaneda S, Nalbantoglu J, Takeishi K, Shimizu K, Gotoh O, Seno T and Ayusawa D: Structural and functional analysis of the human thymidylate synthase gene. J Biol Chem 265: 20277-20284, 1990. (Pubitemid 120014007)
18. Mauritz R, Giovannetti E, Beumer IJ, Smid K, van Groeningen CJ, Pinedo HM and Peters GJ: Polymorphisms in the enhancer region of the thymidylate synthase gene are associated with thymidylate synthase levels in normal tissues but not in malignant tissues of patients with colorectal cancer. Clin Colorectal Cancer 8: 146-154, 2009.
19. Subbarayan PR, Lima M and Ardalan B: Arsenic trioxide/ascorbic acid therapy in patients with refractory metastatic colorectal carcinoma: a clinical experience. Acta Oncol 46: 557-561, 2007. (Pubitemid 46752248)