Identification of differentially transcribed genes in shrimp Litopenaeus vannamei exposed to osmotic stress and challenged with WSSV virus

Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

Volumn 7, Issue 1, 2012, Pages 73-81

  Gonçalves Soares, Daniela ^a   Seiffert, Walter Quadros ^b   Schlindwein, Aline Daiane ^c   Toledo Silva, Guilherme ^d   Zanette, Juliano ^e   Marques, Maria Risoleta Freire ^a   Bainy, Afonso Celso Dias ^a  

^a Lab Biomarcadores de Contaminação Aquática e Imunoquímica   (Brazil)

^b Depto Aquicultura   (Brazil)

^c FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

^d UNIVERSITY OF CAMPINAS   (Brazil)

^e FEDERAL UNIVERSITY OF RIO GRANDE   (Brazil)

Author keywords

Gill; Litopenaeus vannamei; Osmotic stress; qPCR; SSH; WSSV

Indexed keywords

CELL PROTEIN; CICLOPHILIN; LECTIN; LECTIN C; MALATE DEHYDROGENASE; NUCLEAR PROTEIN; PROTEIN QM; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; TRANSFORMING GROWTH FACTOR BETA INDUCIBLE NUCLEAR PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CELL DIFFERENTIATION; CELL METABOLISM; CELL PROLIFERATION; CYTOSKELETON; DOWN REGULATION; ELECTRON TRANSPORT; GENE CONTROL; GENE IDENTIFICATION; GENETIC TRANSCRIPTION; LITOPENAEUS VANNAMEI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OSMOTIC STRESS; PH MEASUREMENT; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SHRIMP; STRESS; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; SURVIVAL RATE; UPREGULATION; WATER QUALITY; WHITE SPOT SYNDROME VIRUS;

ANIMALIA; DECAPODA (CRUSTACEA); LITOPENAEUS VANNAMEI; SHRIMP WHITE SPOT SYNDROME VIRUS;

EID: 84855250563     PISSN: 1744117X     EISSN: 18780407     Source Type: Journal    
DOI: 10.1016/j.cbd.2011.11.002     Document Type: Article

References (92)

1. S.F. Altschul, T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res. 25 1997 3389 3402 (Pubitemid 27359211)
2. H.L. Atwood, S.P. Young, J.R. Tomasso, and C.L. Browdy Survival and growth of pacific white shrimp Litopenaeus vannamei postlarvae in low-salinity and mixed-salt environments J. World Aquac. Soc. 34 2003 518 523 (Pubitemid 38092886)
3. K. Böttcher, D. Siebers, and W. Becker Carbonic anhydrase in branchial tissues of osmoregulating shore crabs, Carcinus maenas J. Exp. Zool. 255 1990 251 261 (Pubitemid 20305903)
4. C. Boyd Water Quality in Pond for Aquaculture 1990 Auburn University, Alabama, Birmingham, Publishing Co Alabama 482p
5. W.A. Bray, A.L. Lawrence, and J.R. Leung-Trujillo The effect of salinity on growth and survival of Penaeus vannamei, with observations on the interaction of IHHN virus and salinity Aquaculture 122 1994 133 146
6. D. Buranajitpirom, S. Asuvapongpatana, W. Weerachatyanukul, K. Wongprasert, W. Namwong, P. Poltana, and B. Withyachumnarnkul Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland Cell Tissue Res. 340 2010 481 489
7. F.L. Castille Jr., and A.L. Lawrence The effect of salinity on the osmotic, sodium and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus Comp. Biochem. Physiol. A 68 1981 75 80
8. G. Charmantier, N. Bouaricha, M. Charmantier-Daures, P. Thuet, and J.P. Trilles Salinity tolerance and osmoregulatory capacity as indicators of the physiological state of penaeid shrimps Eur. Aquat. Soc. Spec. Publ. 10 1989 65 66
9. J.R. Chávez Parametros quimicos usados en acuacultura para agua dulce y agua salada Industria Acuicola 5 2009 10
10. J.H. Chen, and I.C. Liao The effect of salinity on the osmotic and ionic concentrations in the hemolymph of Penaeus monodon and P. penicillatus I.L. Maclean, L.B. Dizon, L.V. Hosillos, The First Asian Fisheries Forum, Asian Fisheries Society, Manila 1986 633 636
11. A. Conesa, S. Götz, J.M. García-Gómez, J. Terol, M. Talón, and M. Robles Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research Bioinformatics 21 2005 3674 3676 (Pubitemid 41264107)
12. T.A. Coote, P.W. Hone, R. Kenyon, and G.B. Maguire The effect of different combinations of dietary calcium and phosphorus on the growth of juvenile Haliotis laevigata Aquaculture 145 1996 267 279 (Pubitemid 26420355)
13. S.W. Costa, L.R.M. Vicente, T.M. de Souza, E.R. Andreatta, and M.R.F. Marques Cultivation parameters and the white spot disease in shrimp farms in Santa Catarina, Brazil [Parâmetros de cultivo e a enfermidade da mancha-branca em fazendas de camarões de Santa Catarina] Pesqui. Agropec. Bras. 45 2010 1521 1530
14. F.H.F. Costa, N.S.M.S. Valença, A.R.B.P. Silva, G.A. Bezerra, B.S. Cavada, and G. Rádis-Baptista Cloning and molecular modeling of Litopenaeus vannamei (Penaeidae) C-type lectin homologs with mutated mannose binding domain-2 Genet. Mol. Res. 10 2011 650 664
15. D.A. Davis, and D.M. Gatlin Dietary mineral requirements of fish and marine crustaceans Rev. Fish. Sci. 4 1996 75 99 (Pubitemid 126512365)
16. D.A. Davis, and A.L. Lawrence Minerals L.R. D'Abramo, D.E. Conklin, D.M. Akiyama, Crustacean Nutrition 6 Adv. World Aquacult. 1997 150 163
17. D.A. Davis, C.E. Boyd, D.B. Rouse, and I.P. Saoud Effects of potassium, magnesium and age on growth and survival of Litopenaeus vannamei post-larvae reared in inland low salinity well waters in West Alabama J. World Aquac. Soc. 36 2005 416 419 (Pubitemid 41576720)
18. E. De La Vega, B.M. Degnan, M.R. Hall, and K.J. Wilson Differential expression of immune-related genes and transposable elements in black tiger shrimp (Penaeus monodon) exposed to a range of environmental stressors Fish Shellfish Immunol. 23 2007 1072 1088 (Pubitemid 47374525)
19. J. De Lorgeril, D. Saulnier, M.G. Janech, Y. Gueguen, and E. Bachère Identification of genes that are differentially expressed in hemocytes of the Pacific blue shrimp (Litopenaeus stylirostris) surviving an infection with Vibrio penaeicida Physiol. Genomics 21 2005 174 183 (Pubitemid 40898477)
20. O. Decamp, J. Cody, L. Conquest, G. Delanoy, and A.G.J. Tacon Effect of salinity on natural community and production of Litopenaeus vannamei (Boone), within experimental zero-water exchange culture systems Aquac. Res. 34 2003 345 355 (Pubitemid 36463383)
21. O. Deshimaru, and Y. Yone Requirement of prawn for dietary minerals Bull. Jpn. Soc. Sci. Fish. 44 1978 907 910
22. S.F. Dowdy, K.-M. Lai, B.E. Weissman, Y. Matsui, B.L.M. Hogan, and E.J. Stanbridge The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells Nucleic Acids Res. 19 1991 5763 5769 (Pubitemid 21912986)
23. K. Drickamer, and M.E. Taylor Biology of animal lectins Annu. Rev. Cell Biol. 9 1993 237 264
24. EPAGRI Síntese Anual da Agricultura de Santa Catarina 2010 Epagri/Cepa Florianópolis 311 pp.
25. B. Ewing, and P. Green Base-calling of automated sequencer traces using phred. II. Error probabilities Genome Res. 8 1998 186 194 (Pubitemid 28177230)
26. R.P. Ferraris, F.D. Parado-Estepa, J.M. Ladja, and E.G. de Jesus Effect of salinity on the osmotic, chloride, total protein and calcium concentrations in the hemolymph of the prawn Peneaus monodon (Fabricius) Comp. Biochem. Physiol. A 83 1986 701 708
27. N.C. Ferreira, C. Bonetti, and W.Q. Seiffert Hydrological and water quality indices as management tools in marine shrimp culture Aquaculture 318 2011 425 433
28. L.A. Fieber, and P.L. Lutz Calcium requirements for molting in Macrobrachium rosenbergii J. World Maric. Soc. 13 1982 21 27
29. D.S. Fielder, W.J. Bardsley, and G.L. Allan Survival and growth of Australian snapper, Pagrus auratus, in saline groundwater from inland New South Wales, Australia Aquaculture 201 2001 73 90 (Pubitemid 32901226)
30. C.A. Freire, H. Onken, and J.C. McNamara A structure-function analysis of ion transport in crustacean gills and excretory organs Comp. Biochem. Physiol. A 151 2008 272 304
31. J.C. García, A. Reyes, M. Salazar, and C.B. Granja Differential gene expression in White Spot Syndrome Virus (WSSV)-infected naïve and previously challenged Pacific white shrimp Penaeus (Litopenaeus) vannamei Aquaculture 289 2009 253 258
32. H. Green, A.E. Canfield, M.C. Hillarby, M.E. Grant, R.P. Boot-Handford, A.J. Freemont, and G.A. Wallis The ribosomal protein QM is expressed differentially during vertebrate endochondral bone development J. Bone Miner. Res. 5 2000 1066 1075 (Pubitemid 30350069)
33. Y. Guan, Z. Yu, and C. Li The effects of temperature on white spot syndorme infections in Marsupenaeus japonicus J. Invertebr. Pathol. 83 2003 257 260 (Pubitemid 36953228)
34. N. He, H. Liu, and X. Xu Identification of genes involved in the response of haemocytes of Penaeus japonicus by suppression subtractive hybridization (SSH) following microbial challenge Fish Shellfish Immunol. 17 2004 121 128 (Pubitemid 38847823)
35. R.P. Henry The role of carbonic anhydrase in blood ion and acid-base regulation Integr. Comp. Biol. 24 1984 241 251
36. R.P. Henry, and S.A. Watts Early carbonic anhydrase induction in the gills of the blue crab, Callinectes sapidus, during low salinity acclimation is independent of ornithine decarboxylase activity J. Exp. Zool. 289 2001 350 358 (Pubitemid 32437220)
37. P. Jiravanichpaisal, K. Söderhäll, and I. Söderhäll Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish Fish Shellfish Immunol. 17 2004 265 275 (Pubitemid 38991660)
38. P. Jiravanichpaisal, K. Söderhäll, and I. Söderhäll Characterization of white spot sundrome virus replication in vitro-cultured haematopoietic stem cells of freshwater crayfish, Pacifastacus leniusculus J. Gen. Virol. 87 2006 847 854
39. T. Jomori, and S. Natori Function of the lipopolysaccharide-binding protein of Periplaneta americana as an opsonin FEBS Lett. 29 1992 283 286
40. A. Joseph, and R. Philip Acute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infection Aquaculture 272 2007 87 97 (Pubitemid 350046309)
41. R. Kalendar, D. Lee, and A.H. Schulman FastPCR software for PCR primer and probe design and repeat search Genes Genomes Genomics 3 2009 1 14
42. N. Kautsky, P. Rönnbäck, M. Tedengren, and M. Troell Ecosystem perspectives on management of disease in shrimp pond farming Aquaculture 191 2000 145 161
43. N. Koizumi, M. Imamura, T. Kadotani, K. Yaoi, H. Iwahana, and R. Sato The lipopolysaccharide-binding protein participating in hemocyte nodule formation in the silkworm Bombyx mori is a novel member of the C-type lectin superfamily with two different tandem carbohydrate-recognition domains FEBS Lett. 443 1999 139 143 (Pubitemid 29078619)
44. M. Kumlu, O.T. Eroldogan, and M. Aktas The effect of salinity on larval growth, survival and development of Penaeus semisulcatus (Decapoda: Penaeidae) Isr. J. Aquac.Bamid. 51 1999 114 121 (Pubitemid 30203312)
45. M.J. Lieber, and S. Luckhart Transforming growth factor-βs and related gene products in mosquito vectors of human malaria parasites: signaling architecture for immunological crosstalk Mol. Immunol. 41 2004 965 977 (Pubitemid 39070325)
46. S.G. Lillico, J.C. Mottram, N.B. Murphy, and S.C. Welburn Characterisation of the QM gene of Trypanosoma brucei FEMS Microbiol. Lett. 211 2002 123 128 (Pubitemid 34655601)
47. S.-C. Lin, C.-H. Liou, and J.-H. Cheng The role of the antennal glands in ion and body volume regulation of cannulated Penaeus monodon reared in various salinity conditions Comp. Biochem. Physiol. A 127 2000 121 129
48. B. Liu, Z. Yu, X. Song, Y. Guan, X. Jian, and J. He The effect of acute salinity change on white spot syndrome (WSS) outbreaks in Fenneropenaeus chinensis Aquaculture 253 2006 163 170
49. C.F. Lo, J.H. Leu, C.H. Ho, C.H. Chen, S.E. Peng, Y.T. Chen, C.M. Chou, P.Y. Yeh, C.J. Huang, H.Y. Chou, C.H. Wang, and G.H. Kou Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction Dis. Aquat. Org. 25 1996 133 141
50. L.H. Mantel, and L.L. Farmer Osmotic and ionic regulation L.H. Mantel, The Biology of Crustacea 5, Internal Anatomy and Physiological Regulation 1983 Academic Press New York, USA 54 162
51. I. Marty, C. Brugidou, Y. Chartier, and Y. Meyer Growth-related gene expression in Nicotiana tabacum mesophyll protoplasts Plant J. 4 1993 265 278
52. W.J. McGraw, D.A. Davis, D. Teichert-Coddington, and D.B. Rouse Acclimation of Litopenaeus vannamei postlarvae to low salinity: influence of age, salinity endpoint, and rate of salinity reduction J. World Aquac. Soc. 33 2002 78 84 (Pubitemid 34444134)
53. N.M. Morrissey Report on marron in farm dams Fish Report Western Australia 5 1970 1 34
54. Müller, I.C., 2009. Genes diferencialmente expressos em camarões de cultivo Litopenaeus vannamei infectados pelo vírus da Síndrome da Mancha Branca e genotipagem de isolados geográficos brasileiros do vírus., 150p. Tese (Doutorado em Biotecnologia) - Curso de Pós-Graduação em Biotecnologia, Universidade Federal de Santa Catarina, Florianópolis, SC
55. A. Muramoto Endocrine control of water balance in decapod crustaceans H. Laufer, R.G.H. Downer, Endocrinology of Selected Invertebrate Types vol. II 1988 A. R. Liss New York 341 356
56. H.R. Nicholas, and J. Hodgkin Responses to infection and possible recognition strategies in the innate immune system of Caenorhabditis elegans Mol. Immunol. 41 2004 479 493 (Pubitemid 38746658)
57. A.J.P. Nunes, T.C.V. Gesteira, G.G. Oliveira, R.C. Lima, P.T.C. Miranda, and R.M. Madrid Princípios para boas práticas de manejo (BPM) na engorda de camarão marinhos no Estado do Ceará. Instituto de Ciências do Mar (Labomar/UFC) Programa de Zoneamento Ecológico Econômico (ZEE) do Estado do Ceará, Fortaleza, Ceará 2005 109 pp.
58. C. Oh, M. Zoysa, C. Nikapitiya, I. Whang, Y.C. Kim, D.H. Kang, S.J. Heo, Y.U. Choi, C.Y. Choi, J.S. Lee, and J. Lee Tumor suppressor QM-like gene from disk abalone (Haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge Fish Shellfish Immunol. 29 2010 494 500
59. D. Pan, N. He, Z. Yang, H. Liu, and X. Xu Difeferential gene expression profile in hepatopancreas of WSSV-resistant shrimp (Penaeus japonicus) by supression substrative hybridization Dev. Comp. Immunol. 29 2005 103 112 (Pubitemid 39265091)
60. L.M. Passano Moulting and its control T.H. Waterman, Physiology of Crustacea I 1960 Academic Press New York 473 536
61. L.I. Peinado-Guevara, and M. LoÍpez-Meyer Detailed monitoring of white spot syndrome virus (WSSV) in shrimp commercial ponds in Sinaloa, Mexico by nested PCR Aquaculture 251 2006 33 45 (Pubitemid 41820942)
62. A. Péqueux Osmotic regulation in crustaceans J. Crust. Biol. 15 1995 1 60
63. M.W. Pfaffl, G.W. Horgan, and L. Dempfle Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR Nucleic Acids Res. 30 2002 e36
64. S. Pongsomboon, S. Udomlertpreecha, P. Amparyup, S. Wuthisuthimethavee, and A. Tassanakajon Gene expression and activity of carbonic anhydrase in salinity stressed Penaeus monodon Comp. Biochem. Physiol. 152 2009 225 233
65. A. Prapavorarat, S. Pongsomboon, and A. Tassanakajon Identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, Penaeus monodon, by suppression subtractive hybridization Dev. Comp. Immunol. 34 2010 611 617
66. S. Prior, C.L. Browdy, E.F. Shepard, R. Laramore, and P.G. Parnell Controlled bioassay systems for determination of lethal infective doses of tissue homogenates containing Taura syndrome or white spot syndrome virus Dis. Aquat. Organ. 54 2003 89 96 (Pubitemid 36521745)
67. M.M. Rahman, C.M. Escobedo-Bonilla, M. Corteel, J.J. Dantas-Lima, M. Wille, V.A. Sanz, M.B. Pensaert, P. Sorgeloos, and H.J. Nauwynck Effect of high water temperature (33 C) on the clinical and virological outcome of experimental infections with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Litopenaeus vannamei Aquaculture 261 2006 842 849 (Pubitemid 44666965)
68. A. Reyes, M. Salazar, and C. Granja Temperature modifies gene expression in subcuticular epithelial cells of white spot syndrome virus-infected Litopenaeus vannamei Dev. Comp. Immunol. 31 2007 23 29 (Pubitemid 44436566)
69. L. Robertson, W. Bray, J.L. Trujillo, and A. Lawrence Practical molt staging of Penaeus setiferus and Penaeus stylirostris J. World Aquac. Soc. 18 1987 180 185
70. S. Rozen, and H.J. Skaletsky Primer3 on the WWW for general users and for biologist programmers S. Krawetz, S. Misener, Bioinformatics Methods and Protocols: Methods in Molecular Biology 2000 Humana Press Totowa, NJ 365 386
71. J.M.J. Ruiz-Velazco, A. Hernández-Llamas, V.M. Gomez-Muñoz, and F.J. Magallon Dynamics of intensive production of shrimp Litopenaeus vannamei affected by white spot disease Aquaculture 300 2010 113 119
72. T.M. Samocha, H. Guajardo, A.L. Lawrence, F.L. Castille, M. Speed, D.A. McKee, and K.I. Page A simple stress test for Penaeus vannamei postlarvae Aquaculture 165 1998 233 242 (Pubitemid 28395340)
73. T.M. Samocha, L. Hamper, C.R. Emberson, A.D. Davis, D. McIntosh, A.L. Lawrence, and P.M. Van Wyk Review of some recent developments in sustainable shrimp farming practices in Texas, Arizona, and Florida J. Appl. Aquac. 12 2002 1 42 (Pubitemid 34268070)
74. I.P. Saoud, D.A. Davis, and D.B. Rouse Suitability studies of inland well waters for Litopenaeus vannamei culture Aquaculture 217 2003 373 383 (Pubitemid 36219204)
75. W.Q. Seiffert A Mancha branca em Santa Catarina Panorama da Aquicultura 15 2005 51 53
76. U. Tantulo, and R. Fotedar Osmo and ionic regulation of black tiger prawn (Penaeus monodon Fabricius 1798) juveniles exposed to K + deficient inland saline water at different salinities Comp. Biochem. Physiol. A 146 2007 208 214 (Pubitemid 46187115)
77. L. Tapay, C. Nadala, and P. Loh A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus J. Virol. Methods 82 1999 39 43 (Pubitemid 29415710)
78. E.A. Tendencia, R.H. Bosma, and J.A.J. Verreth WSSV risk factors related to water physico-chemical properties and microflora in semi-intensive Penaeus monodon culture ponds in the Philippines Aquaculture 302 2010 164 168
79. D.W. Towle, R.P. Henry, and N.B. Terwilliger Microarray-detected changes in gene expression in gills of green crabs (Carcinus maenas) upon dilution of environmental salinity Comp. Biochem. Physiol. D 6 2011 115 125
80. M.F. Tsai, G.H. Kou, H.C. Liu, K.F. Liu, C.F. Chang, S.E. Peng, H.C. Hsu, C.H. Wang, and C.F. Lo Long-term presence of white spot syndrome virus (WSSV) in a cultivated shrimp population without disease outbreaks Dis. Aquat. Organ. 38 1993 107 114
81. O.M. Vidal, C. Granja, F. Aranguren, J. Brock, and M. Salazar A profound effect of hyperthermia on survival of Litopenaeus vannamei juveniles infected with white spot syndrome virus J. World Aquac. Soc. 32 2001 364 372 (Pubitemid 34137625)
82. K.K. Vijayan, and A.D. Diwan Fluctuations in Ca, Mg and P levels in the hemolymph, muscle, midgut gland and exoskeleton during the moult cycle of the Indian white prawn, Penaeus indicus (Decapoda: Penaeidae) Comp. Biochem. Physiol. A 114 1996 91 97 (Pubitemid 26188016)
83. F.-I. Wang, and J.-C. Chen The immune response of tiger shrimp Penaeus monodon and its susceptibility to Photobacterium damselae subsp. damselae under temperature stress Aquaculture 258 2006 34 41 (Pubitemid 44205231)
84. Q. Wang, B. White, R. Redman, and D. Lightner Per os challenge of Litopenaeus vannamei postlarvae and Farfantepenaeus duodorum juveniles with six geographic isolares of white spot syndrome virus Aquaculture 170 1999 179 194 (Pubitemid 29032254)
85. D. Weihrauch, A. Ziegler, D. Siebers, and D.W. Towle Molecular characterization of V-type H(+)-ATPase (B-subunit) in gills of euryhaline crabs and its physiological role in osmoregulatory ion uptake J. Exp. Biol. 204 2001 25 37 (Pubitemid 32106341)
86. V.P. Wyk Farming marine shrimp in freshwater systems: an economic development strategy for Florida Final Report 1999 Harbor Branch Oceonographic Institution
87. J. Xu, S. Wu, and X. Zhang Novel function of QM protein of shrimp (Penaeus japonicus) in regulation of phenol oxidase activity by interaction with hemocyanin Cell. Physiol. Biochem. 21 2008 473 480 (Pubitemid 351620301)
88. X.-Q. Yu, and M.R. Kanost Immulectin-2, a lipopolysaccharide-specific lectin from an insect, Manduca sexta, is induced in response to Gram-negative bacteria J. Biol. Chem. 275 2000 37373 37381 (Pubitemid 32004840)
89. X.-Q. Yu, and M.R. Kanost Manduca sexta lipopolysaccharide-specific immulectin-2 protects larvae from bacterial infection Dev. Comp. Immunol. 27 2003 189 196 (Pubitemid 36184619)
90. Z. Yu, C. Li, and Y. Guan Effect of salinity on the immune responses and outbreak of white spot syndrome in the shrimp Marsupenaeus japonicus Ophelia 57 2003 99 106 (Pubitemid 37089904)
91. Z.-Y. Zhao, Z.-X. Yin, S.-P. Weng, H.-J. Guan, S.-D. Li, K. Xing, S.-M. Chan, and J.-G. He Profiling of differentially expressed genes in hepatopancreas of white spot syndrome virus-resistant shrimp (Litopenaeus vannamei) by suppression subtractive hybridisation Fish Shellfish Immunol. 22 2007 520 534 (Pubitemid 46178487)
92. Z.-Y. Zhao, Z.-X. Yin, X.-P. Xu, S.-P. Weng, X.-Y. Rao, Z.-X. Dai, Y.-W. Luo, G. Yang, Z.-Z. Li, H.-J. Guan, S.-D. Li, S.-M. Chan, X.-Q. Yu, and J.-G. He A novel C-type lectin from the shrimp Litopenaeus vannamei possesses anti-white spot syndrome virus activity J. Virol. 83 2009 347 356