Trichoderma reesei RUT-C30 - Thirty years of strain improvement

Microbiology

Volumn 158, Issue 1, 2012, Pages 58-68

  Peterson, Robyn ^a   Nevalainen, Helena ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; MESSENGER RNA; RECOMBINANT PROTEIN;

CATABOLITE DEREPRESSION; CATABOLITE REPRESSION; CELL VIABILITY; CELLULOLYTIC ACTIVITY; CHEMICAL ANALYSIS; ENZYME MECHANISM; ENZYME SYNTHESIS; FUNGAL METABOLISM; FUNGAL STRAIN; GENE DELETION; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC STRAIN; GENOME ANALYSIS; GENOMICS; GLYCOBIOLOGY; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; HYPOCREA JECORINA; MICROSCOPY; MOULD; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEOMICS; REVIEW; STRAIN IMPROVEMENT; TRANSCRIPTOMICS; WILD TYPE;

FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENETIC ENGINEERING; GENOMICS; HISTORY, 20TH CENTURY; HISTORY, 21ST CENTURY; TRICHODERMA;

FUNGI; HYPOCREA JECORINA;

EID: 84855228527     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.054031-0     Document Type: Review

References (98)

1. Arvas, M., Pakula, T., Lanthaler, K., Saloheimo, M., Valkonen, M., Suortti, T., Robson, G. & Penttilä, M. (2006). Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae. BMC Genomics 7, 32.
2. Asadollahi, M. A., Maury, J., Patil, K. R., Schalk, M., Clark, A. & Nielsen, J. (2009). Enhancing sesquiterpene production in Saccharomyces cerevisiae through in silico driven metabolic engineering. Metab Eng 11, 328-334.
3. Bailey, M. J. &Tähtiharju, J. (2003). Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy. Appl Microbiol Biotechnol 62,156-162.
4. Bergquist, P., Téo, V., Gibbs, M., Cziferszky, A., de Faria, F. P., Azevedo, M. & Nevalainen, H. (2002). Expression of xylanase enzymes from thermophilic microorganisms in fungal hosts. Extremophiles 6, 177-184.
5. Bisaria, V. S. & Ghose, T. K. (1981). Biodegradation of cellulosic materials: substrates, microorganisms, enzymes and products. Enzyme Microb Technol 3, 90-104.
6. Boghigian, B. A., Seth, G., Kiss, R. & Pfeifer, B. A. (2010). Metabolic flux analysis and pharmaceutical production. Metab Eng 12, 81- 95.
7. Brody, H. & Maiyuran, S. (2009). RNAi-mediated gene silencing of highly expressed genes in the industrial fungi Trichoderma reesei and Aspergillus niger. Ind Biotechnol 5, 53-60.
8. Carberry, S. & Doyle, S. (2007). Proteomic studies in biomedically and industrially relevant fungi. Cytotechnology 53, 95-100.
9. Carter, G. L., Allison, D., Rey, M. W. & Dunn-Coleman, N. S. (1992). Chromosomal and genetic analysis of the electrophoretic karyotype of Trichoderma reesei: mapping of the cellulase and xylanase genes. Mol Microbiol 6, 2167-2174.
10. Cherry, J. R. & Fidantsef, A. L. (2003). Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 14, 438-443.
11. Choy, V., Patel, N. & Thibault, J. (2011). Application of image analysis in the fungal fermentation of Trichoderma reesei RUT-C30. Biotechnol Prog doi:10.1002/btpr.667.
12. Collén, A., Saloheimo, M., Bailey, M., Penttilä, M. & Pakula, T. M. (2005). Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag. Biotechnol Bioeng 89, 335-344.
13. Datema, R. & Schwarz, R. T. (1978). Formation of 2-deoxyglucosecontaining lipid-linked oligosaccharides. Interference with glycosylation of glycoproteins. Eur J Biochem 90, 505-516.
14. De Bruyn, A., Maras, M., Schraml, J., Herdewijn, P. & Contreras, R. (1997). NMR evidence for a novel asparagine-linked oligosaccharide on cellobiohydrolase I from Trichoderma reesei RUTC 30. FEBS Lett 405, 111-113.
15. de Faria, F. P., Téo, V. S. J., Bergquist, P. L., Azevedo, M. O. & Nevalainen, K. M. H. (2002). Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei. Lett Appl Microbiol 34, 119-123.
16. Eveleigh, D. E. (1982). Reducing the cost of cellulase production - selection of the hypercellulolytic Trichoderma reesei RUT-C30 mutant. New Brunswick: Rutgers University.
17. Fernandes, L., Araújo, M. A., Amaral, A., Reis, V. C. B., Martins, N. F. & Felipe, M. S. (2005). Cell signaling pathways in Paracoccidioides brasiliensis - inferred from comparisons with other fungi. Genet Mol Res 4, 216-231.
18. Gallo, B. J., Andreotti, R., Roche, C., Ryu, D. & Mandels, M. (1978). Cellulase production by a new mutant strain of Trichoderma reesei MCG 77. Biotechnol Bioeng Symp 8, 89-101.
19. Garía, R., Cremata, J. A., Quintero, O., Montesino, R., Benkestock, K.Stålhlberg, J. (2001). Characterization of protein glycoforms with Nlinked neutral and phosphorylated oligosaccharides: studies on the glycosylation of endoglucanase 1 (Cel7B) from Trichoderma reesei. Biotechnol Appl Biochem 33, 141-152.
20. Gerngross, T. U. (2004). Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat Biotechnol 22, 1409-1414.
21. Geysens, S., Pakula, T., Uusitalo, J., Dewerte, I., Penttilä, M. & Contreras, R. (2005). Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30. Appl Environ Microbiol 71, 2910-2924.
22. Ghosh, A., Al-Rabiai, S., Ghosh, B. K., Trimiño-Vazquez, H., Eveleigh, D. E. & Montenecourt, B. S. (1982). Increased endoplasmic reticulum content of a mutant of Trichoderma reesei (RUT-C30) in relation to cellulase synthesis. Enzyme Microb Technol 4, 110-113.
23. Ghosh, A., Ghosh, B. K., Trimino-Vazquez, H., Eveleigh, D. E. & Montenecourt, B. S. (1984). Cellulase secretion from a hypercellulolytic mutant of Trichoderma reesei Rut-C30. Arch Microbiol 140, 126-133.
24. Glenn, M., Ghosh, A. & Ghosh, B. K. (1985). Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction. Appl Environ Microbiol 50, 1137-1143.
25. González-Fernández, R., Prats, E. & Jorrín-Novo, J. V. (2010). Proteomics of plant pathogenic fungi. J Biomed Biotechnol 2010, 932527.
26. Guangtao, Z., Hartl, L., Schuster, A., Polak, S., Schmoll, M., Wang, T., Seidl, V. & Seiboth, B. (2009). Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina. J Biotechnol 139, 146-151.
27. Gyalai-Korpos, M., Mangel, R., Alvira, P., Dienes, D., Ballesteros, M. & Re ́ czey, K. (2011). Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes. J Ind Microbiol Biotechnol 38, 791-802.
28. Harkki, A., Uusitalo, J., Bailey, M., Penttilä, M. & Knowles, J. K. C. (1989). A novel fungal expression system: secretion of active calf chymosin from the filamentous fungus Trichoderma reesei. Nat Biotechnol 7, 596-603.
29. Harrison, M. J., Wathugala, I. M., Tenkanen, M., Packer, N. H. & Nevalainen, K. M. H. (2002). Glycosylation of acetylxylan esterase from Trichoderma reesei. Glycobiology 12, 291-298.
30. Hartl, L. & Seiboth, B. (2005). Sequential gene deletions in Hypocrea jecorina using a single blaster cassette. Curr Genet 48, 204-211.
31. Hazell, B. W., Téo, V. S. J., Bradner, J. R., Bergquist, P. L. & Nevalainen, K. M. H. (2000). Rapid transformation of high cellulaseproducing mutant strains of Trichoderma reesei by microprojectile bombardment. Lett Appl Microbiol 30, 282-286.
32. Herpoël-Gimbert, I., Margeot, A., Dolla, A., Jan, G., Mollé, D., Lignon, S., Mathis, H., Sigoillot, J.-C., Monot, F. & Asther, M. (2008). Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains. Biotechnol Biofuels 1, 18.
33. Hubbard, S. C. & Ivatt, R. J. (1981). Synthesis and processing of asparagine-linked oligosaccharides. Annu Rev Biochem 50, 555-583.
34. Hui, J. P. M., Lanthier, P., White, T. C., McHugh, S. G., Yaguchi, M., Roy, R. & Thibault, P. (2001). Characterization of cellobiohydrolase I (Cel7A) glycoforms from extracts of Trichoderma reesei using capillary isoelectric focusing and electrospray mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 752, 349-368.
35. Ilmén, M., Onnela, M. L., Klemsdal, S., Keränen, S. & Penttilä, M. (1996). Functional analysis of the cellobiohydrolase I promoter of the filamentous fungus Trichoderma reesei. Mol Gen Genet 253, 303-314.
36. Jouhten, P., Pitkänen, E., Pakula, T., Saloheimo, M., Penttilä, M. & Maaheimo, H. (2009). 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose. BMC Syst Biol 3, 104.
37. Joutsjoki, V. V., Torkkeli, T. K. & Nevalainen, K. M. H. (1993a). Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei. Curr Genet 24, 223-228.
38. Joutsjoki, V. V., Kuittinen, M., Torkkeli, T. K. & Suominen, P. L. (1993b). Secretion of the Hormoconis resinae glucoamylase P enzyme from Trichoderma reesei directed by the natural and the cbh1 gene secretion signal. FEMS Microbiol Lett 112, 281-286.
39. Juhász, T., Szengyel, Z., Szijárto ́, N. & Réczey, K. (2004). Effect of pH on cellulase production of Trichoderma reesei RUT C30. Appl Biochem Biotechnol 113-116, 201-211.
40. Kautto, L. K. (2009). Trichoderma reesei proteasome and genome-wide effects of the expression of mutant cellobiohydrolase I. PhD thesis, Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia. http://minerva.mq.edu.au:8080/vital/ access/manager/Repository/mq:9273
41. Kiiskinen, L.-L., Kruus, K., Bailey, M., Ylösmäki, E., Siika-Aho, M. & Saloheimo, M. (2004). Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme. Microbiology 150, 3065-3074.
42. Kim, Y., Nandakumar, M. P. & Marten, M. R. (2007). Proteomics of filamentous fungi. Trends Biotechnol 25, 395-400.
43. Kubicek, C. P., Mikus, M., Schuster, A., Schmoll, M. & Seiboth, B. (2009). Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina. Biotechnol Biofuels 2, 19.
44. Le Crom, S., Schackwitz, W., Pennacchio, L., Magnuson, J. K., Culley, D. E., Collett, J. R., Martin, J., Druzhinina, I. S., Mathis, H. & other authors (2009). Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing. Proc Natl Acad Sci U S A 106, 16151-16156.
45. Li, B. & Warner, J. R. (1996). Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J Biol Chem 271, 16813-16819.
46. Limón, M. C., &/x&&namegr type=editor&&name&&sname&Pakula&/sname&&x&, &/x&&fname&T.&/fname&&/name&&x&, &/x&&name&&sname&Saloheimo&/sname&&x&, &/x&&fname&M.&/fname&&/name&&x& & &/x&&name&&sname&Penttilä&/sname&&x&, &/x&&fname&M&/fname&&/name&&x&. (2011). The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30. Microb Cell Fact 10, 40.
47. Lubertozzi, D. & Keasling, J. D. (2009). Developing Aspergillus as a host for heterologous expression. Biotechnol Adv 27, 53-75.
48. Manczinger, L. & Ferenczy, L. (1985). Somatic cell fusion of Trichoderma reesei resulting in new genetic combinations. Appl Microbiol Biotechnol 22, 72-76.
49. Mandels, M. & Reese, E. T. (1957). Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals. J Bacteriol 73, 269-278.
50. Mandels, M., Weber, J. & Parizek, R. (1971). Enhanced cellulase production by a mutant of Trichoderma viride. Appl Microbiol 21, 152-154.
51. Mäntylä, A. L., Rossi, K. H., Vanhanen, S. A., Penttilä, M. E., Suominen, P. L. & Nevalainen, K. M. H. (1992). Electrophoretic karyotyping of wild-type and mutant Trichoderma longibrachiatum (reesei) strains. Curr Genet 21, 471-477.
52. Maras, M., De Bruyn, A., Schraml, J., Herdewijn, P., Claeyssens, M., Fiers, W. & Contreras, R. (1997a). Structural characterization of Nlinked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. Eur J Biochem 245, 617-625.
53. Maras, M., Saelens, X., Laroy, W., Piens, K., Claeyssens, M., Fiers, W. & Contreras, R. (1997b). In vitro conversion of the carbohydrate moiety of fungal glycoproteins to mammalian-type oligosaccharides - evidence for N-acetylglucosaminyltransferase-I-accepting glycans from Trichoderma reesei. Eur J Biochem 249, 701-707.
54. Maras, M., De Bruyn, A., Vervecken, W., Uusitalo, J., Penttilä, M., Busson, R., Herdewijn, P. & Contreras, R. (1999). In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei. FEBS Lett 452, 365-370.
55. Margolles-Clark, E., Hayes, C. K., Harman, G. E. & Penttilä, M. (1996). Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei. Appl Environ Microbiol 62, 2145-2151.
56. Margolles-Clark, E., Ihnen, M. & Penttilä, M. (1997). Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources. J Biotechnol 57, 167- 179.
57. Martinez, D., Berka, R. M., Henrissat, B., Saloheimo, M., Arvas, M., Baker, S. E., Chapman, J., Chertkov, O., Coutinho, P. M. & other authors (2008). Genome sequencing and analysis of the biomassdegrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat Biotechnol 26, 553-560.
58. Matsuoka, Y. & Shimizu, K. (2010). Current status of 13C-metabolic flux analysis and future perspectives. Process Biochem 45, 1873-1881.
59. Melzer, G., Esfandabadi, M. E., Franco-Lara, E. & Wittmann, C. (2009). Flux design: In silico design of cell factories based on correlation of pathway fluxes to desired properties. BMC Syst Biol 3, 120.
60. Meyer, V. (2008). Genetic engineering of filamentous fungi-progress, obstacles and future trends. Biotechnol Adv 26, 177-185.
61. Miettinen-Oinonen, A., Torkkeli, T., Paloheimo, M. & Nevalainen, H. (1997). Overexpression of the Aspergillus niger pH 2.5 acid phosphatase gene in a heterologous host Trichoderma reesei. J Biotechnol 58, 13-20.
62. Montenecourt, B. S. (1983). Trichoderma reesei cellulases. Trends Biotechnol 1, 156-161.
63. Montenecourt, B. S. & Eveleigh, D. E. (1977a). Semiquantitative plate assay for determination of cellulase production by Trichoderma viride. Appl Environ Microbiol 33, 178-183.
64. Montenecourt, B. S. & Eveleigh, D. E. (1977b). Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Appl Environ Microbiol 34, 777-782.
65. Montenecourt, B. S. & Eveleigh, D. E. (1979). Selective screening methods for the isolation of high yielding cellulase mutants of Trichoderma reesei. In Hydrolysis of Cellulose: Mechanisms of Enzymatic and Acid Catalysis, Advances in Chemistry Series, 181, pp. 289-301.Edited by R. Brown & L. Jurasek. Washington, DC: American Chemical Society.
66. Murray, P., Aro, N., Collins, C., Grassick, A., Penttilä, M., Saloheimo, M. & Tuohy, M. (2004). Expression in Trichoderma reesei and characterisation of a thermostable family 3 b-glucosidase from the moderately thermophilic fungus Talaromyces emersonii. Protein Expr Purif 38, 248-257.
67. Nagendran, S., Hallen-Adams, H. E., Paper, J. M., Aslam, N. & Walton, J. D. (2009). Reduced genomic potential for secreted plant cell-wall-degrading enzymes in the ectomycorrhizal fungus Amanita bisporigera, based on the secretome of Trichoderma reesei. Fungal Genet Biol 46, 427-435.
68. Nakari-Setälä, T., Paloheimo, M., Kallio, J., Vehmaanperä, J., Penttilä, M. & Saloheimo, M. (2009). Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production. Appl Environ Microbiol 75, 4853-4860.
69. Nakayashiki, H., Hanada, S., Nguyen, B. Q., Kadotani, N., Tosa, Y. & Mayama, S. (2005). RNA silencing as a tool for exploring gene function in ascomycete fungi. Fungal Genet Biol 42, 275-283.
70. Nevalainen, H., Lavygina, I., Neethling, D. & Packer, N. (1995). The biochemical nature of the cell envelope of a high cellulase-secreting mutant differs from that of the Trichoderma reesei wild type. J Biotechnol 42, 53-59.
71. Nykänen, M., Saarelainen, R., Raudaskoski, M., Nevalainen, K. M. H. & Mikkonen, A. (1997). Expression and secretion of barley cysteine endopeptidase B and cellobiohydrolase I in Trichoderma reesei. Appl Environ Microbiol 63, 4929-4937.
72. Nyyssö nen, E., Penttilä, M., Harkki, A., Saloheimo, A., Knowles, J. K. C. & Keränen, S. (1993). Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei. Biotechnology (N Y) 11, 591-595.
73. Olsson, L., Christensen, T. M. I. E., Hansen, K. P. &Palmqvist, E. A. (2003). Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme Microb Technol 33, 612-619.
74. Pakula, T. M., Salonen, K., Uusitalo, J. & Penttilä, M. (2005). The effect of specific growth rate on protein synthesis and secretion in the filamentous fungus Trichoderma reesei. Microbiology 151, 135-143.
75. Paloheimo, M., Mäntylä, A., Kallio, J. & Suominen, P. (2003). Highyield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure. Appl Environ Microbiol 69, 7073-7082.
76. Paloheimo, M., Mäntylä, A., Kallio, J., Puranen, T. & Suominen, P. (2007). Increased production of xylanase by expression of a truncated version of the xyn11A gene from Nonomuraea flexuosa in Trichoderma reesei. Appl Environ Microbiol 73, 3215-3224.
77. Penttilä, M., Nevalainen, H., Rättö, M., Salminen, E. & Knowles, J. (1987). A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61, 155-164.
78. Persson, I., Tjerneld, F. & Hahn-Hägerdal, B. (1991). Fungal cellulolytic enzyme production: A review. Process Biochem 26, 65-74.
79. Peterson, R., Grinyer, J. & Nevalainen, H. (2011). Extracellular hydrolase profiles of fungi isolated from koala faeces invite biotechnological interest. Mycol Prog 10, 207-218.
80. Poidevin, L., Levasseur, A., Paës, G., Navarro, D., Heiss-Blanquet, S., Asther, M. & Record, E. (2009). Heterologous production of the Piromyces equi cinnamoyl esterase in Trichoderma reesei for biotechnological applications. Lett Appl Microbiol 49, 673-678.
81. Ralser, M., Wamelink, M. M., Struys, E. A., Joppich, C., Krobitsch, S., Jakobs, C. & Lehrach, H. (2008). A catabolic block does not sufficiently explain how 2-deoxy-D-glucose inhibits cell growth. Proc Natl Acad Sci U S A 105, 17807-17811.
82. Ruiz-Díez, B. (2002). Strategies for the transformation of filamentous fungi. J Appl Microbiol 92, 189-195.
83. Ryu, D. D. Y. & Mandels, M. (1980). Cellulases: biosynthesis and applications. Enzyme Microb Technol 2, 91-102.
84. Saarelainen, R., Mäntylä, A., Nevalainen, H. & Suominen, P. (1997). Expression of barley endopeptidase B in Trichoderma reesei. Appl Environ Microbiol 63, 4938-4940.
85. Salles, B. C., Téo, V. S., Gibbs, M. D., Bergquist, P. L., Filho, E. X., Ximenes, E. A. & Nevalainen, K. M. H. (2007). Identification of two novel xylanase-encoding genes (xyn5 and xyn6) from Acrophialophora nainiana and heterologous expression of xyn6 in Trichoderma reesei. Biotechnol Lett 29, 1195-1201.
86. Saloheimo, M., Valkonen, M. & Penttilä, M. (2003). Activation mechanisms of the HAC1-mediated unfolded protein response in filamentous fungi. Mol Microbiol 47, 1149-1161.
87. Seidl, V., Gamauf, C., Druzhinina, I. S., Seiboth, B., Hartl, L. & Kubicek, C. P. (2008). The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. BMC Genomics 9, 327.
88. Seidl, V., Seibel, C., Kubicek, C. P. & Schmoll, M. (2009). Sexual development in the industrial workhorse Trichoderma reesei. Proc Natl Acad Sci U S A 106, 13909-13914.
89. Sheir-Neiss, G. & Montenecourt, B. S. (1984). Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations. Appl Microbiol Biotechnol 20, 46-53.
90. Simmons, E. G. (1977). Classification of some cellulase-producing Trichoderma species. In Second International Mycological Congress, Abstracts, pp. 618. Edited by E. G. Simmons. University of South Florida, Tampa, FL.
91. Singh, J. & Tyers, M. (2009). A Rab escort protein integrates the secretion system with TOR signaling and ribosome biogenesis. Genes Dev 23, 1944-1958.
92. Singhania, R. R., Sukumaran, R. K. & Pandey, A. (2007). Improved cellulase production by Trichoderma reesei RUT C30 under SSF through process optimization. Appl Biochem Biotechnol 142, 60-70.
93. Stals, I., Sandra, K., Geysens, S., Contreras, R., Van Beeumen, J. & Claeyssens, M. (2004). Factors influencing glycosylation of Trichoderma reesei cellulases. I: Postsecretorial changes of the Oand N-glycosylation pattern of Cel7A. Glycobiology 14, 713-724.
94. Steiger, M. G., Vitikainen, M., Uskonen, P., Brunner, K., Adam, G., Pakula, T., Penttilä, M., Saloheimo, M., Mach, R. L. & Mach-Aigner, A. R. (2011). Transformation system for Hypocrea jecorina (Trichoderma reesei) that favors homologous integration and employs reusable bidirectionally selectable markers. Appl Environ Microbiol 77, 114-121.
95. Téo, V. S. J., Cziferszky, A. E., Bergquist, P. L. & Nevalainen, K. M. H. (2000). Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei. FEMS Microbiol Lett 190, 13-19
96. Téo, V. S. J., Bergquist, P. L. & Nevalainen, K. M. H. (2002). Biolistic transformation of Trichoderma reesei using the Bio-Rad seven barrels Hepta Adaptor system. J Microbiol Methods 51, 393-399.
97. Travers, K. J., Patil, C. K., Wodicka, L., Lockhart, D. J., Weissman, J. S. & Walter, P. (2000). Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation. Cell 101, 249-258.
98. Wang, T. H., Zhong, Y. H., Huang, W., Liu, T. & You, Y. W. (2005). Antisense inhibition of xylitol dehydrogenase gene, xdh1 from Trichoderma reesei. Lett Appl Microbiol 40, 424-429.