Continuous aqueous two-phase extraction of human antibodies using a packed column

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 880, Issue 1, 2012, Pages 148-156

  Rosa, P A J ^a   Azevedo, A M ^a   Sommerfeld, S ^b   Backer W ^b   Aires Barros, M R ^a  

^a Instituto de Biotecnologia e Bioengenharia Lisboa   (Portugal)

^b BAYER AG   (Germany)

Author keywords

Continuous aqueous two phase extraction; Hold up; Human antibodies; Mass transfer; Packed column

Indexed keywords

AQUEOUS TWO-PHASE EXTRACTION; BATCH EXTRACTION; CHINESE HAMSTER OVARY CELLS; COLUMN PACKING MATERIALS; COLUMN PACKINGS; COUNTER CURRENT; DISPERSED PHASE; EXPERIMENTAL SETUP; HIGHER EFFICIENCY; HOLD UP; HUMAN ANTIBODIES; HUMAN IMMUNOGLOBULIN; IGG RECOVERY; MASS TRANSFER STUDIES; MIXER-SETTLER; PACKED COLUMN; PILOT SCALE; RICH PHASE; TWO PHASE SYSTEMS;

ANTIBODIES; GLYCOLS; IMPURITIES; MASS TRANSFER; MIXERS (MACHINERY); POLYETHYLENE GLYCOLS; STAINLESS STEEL;

SOLVENT EXTRACTION;

IMMUNOGLOBULIN G ANTIBODY; MACROGOL 3350; PHOSPHATE; STAINLESS STEEL;

ANIMAL CELL; ANTIBODY ISOLATION; ARTICLE; CHO CELL; CONTINUOUS COUNTER CURRENT AQUEOUS TWO PHASE EXTRACTION; CONTROLLED STUDY; DISPERSION; HYDRODYNAMICS; MIXER; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; SUPERNATANT;

ANIMALS; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CRICETINAE; CRICETULUS; EQUIPMENT DESIGN; HUMANS; HYDRODYNAMICS; IMMUNOGLOBULIN G; LIQUID-LIQUID EXTRACTION; MODELS, THEORETICAL; POLYETHYLENE GLYCOLS; SODIUM CHLORIDE;

CRICETULUS GRISEUS;

EID: 84855189061     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.11.034     Document Type: Article

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