Application of aqueous two-phase systems to antibody purification: A multi-stage approach

Journal of Biotechnology

Volumn 139, Issue 4, 2009, Pages 306-313

  Rosa, P A J ^a   Azevedo, A M ^a   Sommerfeld, S ^b   Mutter, M ^b   Aires Barros, M R ^a   Backer W ^b  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b BAYER AG   (Germany)

Author keywords

Aqueous two phase systems; Fractionation; Human antibodies; McCabe Thiele; Multi stage equilibrium aqueous two phase extraction

Indexed keywords

ANTIBODY CONCENTRATIONS; ANTIBODY PURIFICATIONS; AQUEOUS TWO-PHASE SYSTEMS; CHINESE HAMSTER OVARY CELLS; CHO CELLS SUPERNATANT; COUNTER CURRENTS; EQUILIBRIUM CURVES; EXPERIMENTAL CONDITIONS; FEED STOCKS; HUMAN ANTIBODIES; HUMAN IMMUNOGLOBULINS; IGG RECOVERIES; MCCABE THIELE; MULTI-STAGE APPROACHES; MULTI-STAGE EQUILIBRIUM AQUEOUS TWO-PHASE EXTRACTION; NACL CONCENTRATIONS; PH VALUES; PROTEIN PURITIES; RECOVERY YIELDS; SINGLE STAGES; TEST TUBES; VOLUME RATIOS;

ETHYLENE GLYCOL; IONIC STRENGTH; ISOMERS; PH EFFECTS; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PURIFICATION; SODIUM CHLORIDE; SOLVENT EXTRACTION;

ANTIBODIES;

IMMUNOGLOBULIN G ANTIBODY; SODIUM CHLORIDE;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; CHO CELL; CONTROLLED STUDY; INTERMETHOD COMPARISON; IONIC STRENGTH; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PRODUCT RECOVERY; PROTEIN PURIFICATION; SIMULATION; SUPERNATANT;

ANIMALS; ANTIBODIES, MONOCLONAL; CHEMICAL FRACTIONATION; CHO CELLS; CRICETINAE; CRICETULUS; HUMANS; HYDROGEN-ION CONCENTRATION; OSMOLAR CONCENTRATION; POLYETHYLENE GLYCOLS; SODIUM CHLORIDE;

CRICETULUS GRISEUS;

EID: 60549115762     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.01.001     Document Type: Article

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