Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 1, 2012, Pages 283-291

  Shi, Liang ^a   Fang, Xing ^a   Li, Mengjiao ^a   Mu, Dashuai ^a   Ren, Ang ^a   Tan, Qi ^b   Zhao, Mingwen ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Agrobacterium tumefaciens mediated transformation; Fluorescence assay; Ganoderma lucidum; Histochemical staining assay; Promoter

Indexed keywords

AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION; FLUORESCENCE ASSAY; GANODERMA LUCIDUM; HISTOCHEMICAL STAININGS; PROMOTER;

ASSAYS; FLUORESCENCE; GENE EXPRESSION; NUCLEIC ACIDS;

PLANTS (BOTANY);

BACTERIAL DNA; BETA GLUCURONIDASE; ENHANCED GREEN FLUORESCENT PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; PROBIOTIC AGENT; RECOMBINANT PROTEIN; T DNA; T-DNA;

ARTICLE; CHINESE MEDICINE; CYTOLOGY; FUNGAL GENE; GANODERMA LUCIDUM; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; MITOSIS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROMOTER REGION; REPORTER GENE; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM TUMEFACIENS; BASE SEQUENCE; DNA, BACTERIAL; GENE EXPRESSION; GENES, FUNGAL; GENES, REPORTER; GLUCURONIDASE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; GREEN FLUORESCENT PROTEINS; MEDICINE, CHINESE TRADITIONAL; MITOSIS; PROBIOTICS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; REISHI; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; ASCOMYCOTA; BACTERIA (MICROORGANISMS); FUNGI; GANODERMA LUCIDUM;

EID: 84655169723     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0818-z     Document Type: Article

References (27)

1. Ando A, Sumida Y, Negoro H, Suroto DA, Ogawa J, Sakuradani E, Shimizu S (2009) Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding. Appl Environ Microbiol 75(17): 5529-5535.
2. Berbee ML, Taylor JW (1993) Dating the evolutionary radiations of the true fungi. Can J Bot 71(8): 1114-1127.
3. Chen X, Stone M, Schlagnhaufer C, Romaine CP (2000) A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Appl Environ Microbiol 66(10): 4510-4513.
4. Chen EC, Su YH, Kanagarajan S, Agrawal DC, Tsay HS (2009) Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea. Mycol Res 113(Pt 3): 290-297.
5. Combier JP, Melayah D, Raffier C, Gay G, Marmeisse R (2003) Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum. FEMS Microbiol Lett 220(1): 141-148.
6. Covert SF, Kapoor P, Lee M-h, Briley A, Nairn CJ (2001) Agrobacterium tumefaciens-mediated transformation of Fusarium circinatum. Mycol Res 105(03): 259-264.
7. de Groot MJ, Bundock P, Hooykaas PJ, Beijersbergen AG (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16(9): 839-842.
8. Ding YX, Ou-Yang X, Shang CH, Ren A, Shi L, Li YX, Zhao MW (2008) Molecular cloning, characterization, and differential expression of a farnesyl-diphosphate synthase gene from the basidiomycetous fungus Ganoderma lucidum. Biosci Biotechnol Biochem 72(6): 1571-1579.
9. dos Reis MC, Pelegrinelli Fungaro MH, Delgado Duarte RT, Furlaneto L, Furlaneto MC (2004) Agrobacterium tumefaciens-mediated genetic transformation of the entomopathogenic fungus Beauveria bassiana. J Microbiol Methods 58(2): 197-202.
10. Duarte RTD, Staats CC, Fungaro MHP, Schrank A, Vainsten MH, Furlaneto-Maia L, Nakamura CV, de Souza W, Furlaneto MC (2007) Development of a simple and rapid Agrobacterium tumefaciens-mediated transformation system for the entomopathogenic fungus Metarhizium anisopliae var. acridum. Lett Appl Microbiol 44(3): 248-254.
11. Eo SK, Kim YS, Lee CK, Han SS (1999) Antiviral activities of various water and methanol soluble substances isolated from Ganoderma lucidum. J Ethnopharmacol 68(1-3): 129-136.
12. Godio RP, Fouces R, Gudina EJ, Martin JF (2004) Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium. Curr Genet 46(5): 287-294.
13. Kim S, Song J, Choi HT (2004) Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration. FEMS Microbiol Lett 233(2): 201-204.
14. Lin ZB (1979) The current pharmacological research on Ganoderma lucidum in China. Acta Pharmaceutica Sinica (in Chinese) 14(3): 183-192.
15. Meyer V, Mueller D, Strowig T, Stahl U (2003) Comparison of different transformation methods for Aspergillus giganteus. Curr Genet 43(5): 371-377.
16. Michielse CB, Hooykaas PJ, van den Hondel CA, Ram AF (2005) Agrobacterium-mediated transformation as a tool for functional genomics in fungi. Curr Genet 48(1): 1-17.
17. Michielse CB, Hooykaas PJ, van den Hondel CA, Ram AF (2008) Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori. Nat Protoc 3(10): 1671-1678.
18. Mizushina Y, Hanashima L, Yamaguchi T, Takemura M, Sugawara F, Saneyoshi M, Matsukage A, Yoshida S, Sakaguchi K (1998) A mushroom fruiting body-inducing substance inhibits activities of replicative DNA polymerases. Biochem Biophys Res Commun 249(1): 17-22.
19. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81(24): 8014-8018.
20. Shang CH, Zhu F, Li N, Ou-Yang X, Shi L, Zhao MW, Li YX (2008) Cloning and characterization of a gene encoding HMG-CoA reductase from Ganoderma lucidum and its functional identification in yeast. Biosci Biotechnol Biochem 72(5): 1333-1339.
21. Shang CH, Shi L, Ren A, Qin L, Zhao MW (2010) Molecular cloning, characterization, and differential expression of a lanosterol synthase gene from Ganoderma lucidum. Biosci Biotechnol Biochem 74(5): 974-978.
22. Sun L, Cai H, Xu W, Hu Y, Gao Y, Lin Z (2001) Efficient transformation of the medicinal mushroom Ganoderma lucidum. Plant Mol Biol Report 19(4): 383-384.
23. Sun W, Liu L, Hu X, Tang J, Liu P, Chen J, Chen Y (2009) Generation and identification of DNA sequence flanking T-DNA integration site of Trichoderma atroviride mutants with high dichlorvos-degrading capacity. Bioresour Technol 100(23): 5941-5946.
24. Vain P (2007) Thirty years of plant transformation technology development. Plant Biotechnol J 5(2): 221-229.
25. Wang H, Ng TB (2006) Ganodermin, an antifungal protein from fruiting bodies of the medicinal mushroom Ganoderma lucidum. Peptides 27(1): 27-30.
26. Xu F, Zhao MW, Li YX (2006) Cloning and sequence analysis of a glyceraldehyde-3-phosphate dehydrogenase gene from Ganoderma lucidum. J Microbiol 44(5): 515-522.
27. Zhao MW, Liang WQ, Zhang DB, Wang N, Wang CG, Pan YJ (2007) Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum. J Microbiol Biotechnol 17(7): 1106-1112.