Quantitative visualization of autophagy induction by mTOR inhibitors

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 239-250

  Nyfeler, Beat ^a   Bergman, Philip ^a   Wilson, Christopher J ^a   Murphy, Leon O ^a  

^a NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Autophagy; High content imaging; Image recognition algorithm; Ku 0063794; LC3; mTOR; Rapamycin

Indexed keywords

GREEN FLUORESCENT PROTEIN; KU 0063794; LIGHT CHAIN 3, HUMAN; MICROTUBULE ASSOCIATED PROTEIN; MORPHOLINE DERIVATIVE; PYRIMIDINE DERIVATIVE; RAPAMYCIN; TARGET OF RAPAMYCIN KINASE;

AMINO ACID SEQUENCE; ARTICLE; AUTOPHAGY; DRUG ANTAGONISM; DRUG EFFECT; FLUORESCENCE MICROSCOPY; GENE VECTOR; HUMAN; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; AUTOPHAGY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MOLECULAR SEQUENCE DATA; MORPHOLINES; PYRIMIDINES; SIROLIMUS; TOR SERINE-THREONINE KINASES;

MAMMALIA;

EID: 84555187740     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_14     Document Type: Article

References (19)

1. Laplante M, Sabatini DM (2009) mTOR signaling at a glance. J. Cell Sci. 122, 3589-3594.
2. Wullschleger S, Loewith R, Hall MN (2006) TOR signaling in growth and metabolism. Cell 124, 471-484. (Pubitemid 43199434)
3. He C, Klionsky DJ (2009) Regulation mechanisms and signaling pathways of autophagy. Annu. Rev. Genet. 43, 67-93.
4. Klionsky DJ, Codogno P, Cuervo AM et al (2010) A comprehensive glossary of autophagyrelated molecules and processes. Autophagy. 6.
5. Xie Z, Klionsky DJ (2007) Autophagosome formation: core machinery and adaptations. Nat. Cell Biol. 9, 1102-1109. (Pubitemid 47500484)
6. Yang Z, Klionsky DJ (2009) An overview of the molecular mechanism of autophagy. Curr. Top. Microbiol. Immunol. 335, 1-32.
7. Jung CH, Ro SH, Cao J et al (2010) mTOR regulation of autophagy. FEBS Lett. 584, 1287-1295.
8. Rubinsztein DC, Gestwicki JE, Murphy LO et al (2007) Potential therapeutic applications of autophagy. Nat. Rev. Drug Discov. 6, 304-312. (Pubitemid 46505881)
9. Noda T, Ohsumi Y (1998) Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast. J. Biol. Chem. 273, 3963-3966. (Pubitemid 28103257)
10. Ravikumar B, Vacher C, Berger Z et al (2004) Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease. Nat. Genet. 36, 585-595. (Pubitemid 38715985)
11. Mizushima N, Yoshimori T (2007) How to interpret LC3 immunoblotting. Autophagy. 3, 542-545. (Pubitemid 350060049)
12. Kabeya Y, Mizushima N, Ueno T et al (2000) LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19, 5720-5728.
13. Bowman EJ, Siebers A, Altendorf K (1988) Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc. Natl. Acad. Sci. USA 85, 7972-7976.
14. Klionsky DJ, Abeliovich H, Agostinis P et al (2008) Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy. 4, 151-175. (Pubitemid 351231180)
15. Mizushima N, Yoshimori T, Levine B (2010) Methods in mammalian autophagy research. Cell 140, 313-326.
16. Kimura S, Noda T, Yoshimori T (2007) Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescenttagged LC3. Autophagy. 3, 452-460. (Pubitemid 47293726)
17. Pankiv S, Clausen TH, Lamark T et al (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J. Biol. Chem. 282, 24131-24145. (Pubitemid 47328003)
18. Nicklin P, Bergman P, Zhang B et al (2009) Bidirectional transport of amino acids regulates mTOR and autophagy. Cell 136, 521-534.
19. Garcia-Martinez JM, Moran J, Clarke RG et al (2009) Ku-0063794 is a specific inhibitor of the mammalian target of rapamycin (mTOR). Biochem. J. 421, 29-42.