Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 305-316

  Araki, Koichi ^a   Konieczny, Bogumila T ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

FKBP12; In vivo; LCMV; mTOR; Raptor; Retrovirus; RNAi; T cells

Indexed keywords

CARRIER PROTEIN; FK 506 BINDING PROTEIN; RAPTOR PROTEIN, MOUSE; TARGET OF RAPAMYCIN KINASE;

ANIMAL; ARTICLE; C57BL MOUSE; CD8+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL LINE; CYTOLOGY; DISEASE MODEL; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENE SILENCING; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; HUMAN; IMMUNOLOGY; INNATE IMMUNITY; LYMPHOCYTIC CHORIOMENINGITIS; METABOLISM; MOUSE; RETROVIRUS; RNA INTERFERENCE; VIROLOGY;

ANIMALS; CARRIER PROTEINS; CD8-POSITIVE T-LYMPHOCYTES; CELL DIFFERENTIATION; CELL LINE; DISEASE MODELS, ANIMAL; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; GENETIC VECTORS; HUMANS; IMMUNITY, INNATE; LYMPHOCYTIC CHORIOMENINGITIS; MICE; MICE, INBRED C57BL; RETROVIRIDAE; RNA INTERFERENCE; TACROLIMUS BINDING PROTEIN 1A; TOR SERINE-THREONINE KINASES; TRANSDUCTION, GENETIC;

BACTERIA (MICROORGANISMS); LYMPHOCYTIC CHORIOMENINGITIS VIRUS; MAMMALIA; RAPTORES;

EID: 84555187727     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_19     Document Type: Article

References (12)

1. Wullschleger S, Loewith R, Hall MN (2006) TOR signaling in growth and metabolism. Cell 124:471-484 (Pubitemid 43199434)
2. Halloran PF (2004) Immunosuppressive drugs for kidney transplantation. N Engl J Med 351:2715-2729 (Pubitemid 39665325)
3. Araki K, Youngblood B, Ahmed R (2010) The role of mTOR in memory CD8 T-cell differentiation. Immunol Rev 235:234-243
4. Thomson AW, Turnquist HR, Raimondi G (2009) Immunoregulatory functions of mTOR inhibition. Nat Rev Immunol 9:324-337
5. Delgoffe GM, Kole TP, Zheng Y, Zarek PE, Matthews KL, Xiao B, Worley PF, Kozma SC, Powell JD (2009) The mTOR kinase differentially regulates effector and regulatory T cell lineage commitment. Immunity 30:832-844
6. Araki K, Turner AP, Shaffer VO, Gangappa S, Keller SA, Bachmann MF, Larsen CP, Ahmed R (2009) mTOR regulates memory CD8 T-cell differentiation. Nature 460:108-112
7. Pearce EL, Walsh MC, Cejas PJ, Harms GM, Shen H, Wang LS, Jones RG, Choi Y (2009) Enhancing CD8 T-cell memory by modulating fatty acid metabolism. Nature 460: 103-107
8. Rao RR, Li Q, Odunsi K, Shrikant PA (2010) The mTOR Kinase Determines Effector versus Memory CD8(+) T Cell Fate by Regulating the Expression of Transcription Factors T-bet and Eomesodermin. Immunity 32:67-78
9. Hara K, Maruki Y, Long X, Yoshino K, Oshiro N, Hidayat S, Tokunaga C, Avruch J, Yonezawa K (2002) Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action. Cell 110:177-189 (Pubitemid 34876546)
10. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM (2002) mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Cell 110:163-175 (Pubitemid 34876545)
11. Wherry EJ, Teichgraber V, Becker TC, Masopust D, Kaech SM, Antia R, von Andrian UH, Ahmed R (2003) Lineage relationship and protective immunity of memory CD8 T cell subsets. Nat Immunol 4:225-234 (Pubitemid 36322131)
12. Joshi NS, Cui W, Chandele A, Lee HK, Urso DR, Hagman J, Gapin L, Kaech SM (2007) Inflammation directs memory precursor and short-lived effector CD8(+) T cell fates via the graded expression of T-bet transcription factor. Immunity 27:281-295 (Pubitemid 47284924)