Purification and physicochemical characterization of a serine protease with fibrinolytic activity from latex of a medicinal herb Euphorbia hirta

Plant Physiology and Biochemistry

Volumn 52, Issue , 2012, Pages 104-111

  Patel, Girijesh Kumar ^a   Kawale, Ashish Ashok ^a   Sharma, Ashwani Kumar ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY ROORKEE   (India)

Author keywords

CD studies; Euphorbia hirta; Euphorbiaceae; Fibrinogenolytic activity; Fibrinolytic activity; Kinetic studies

Indexed keywords

FIBRIN; FIBRINOGEN; FIBRINOLYTIC AGENT; HIRTIN; LATEX; SERINE PROTEINASE; TRITERPENE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; EUPHORBIA; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; MEDICINAL PLANT; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; TEMPERATURE;

AMINO ACID SEQUENCE; EUPHORBIA; FIBRIN; FIBRINOGEN; FIBRINOLYTIC AGENTS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LATEX; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PLANTS, MEDICINAL; SERINE PROTEASES; SUBSTRATE SPECIFICITY; TEMPERATURE; TRITERPENES;

CHAMAESYCE HIRTA; EUPHORBIACEAE;

EID: 84455178872     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2011.12.004     Document Type: Article

References (37)

1. Lijnen H.R. Matrix metalloproteinases and cellular fibrinolytic activity. Biochemistry (Moscow) 2002, 67:92-98.
2. Berkner K.L. Blood Clotting: General Pathway, Encyclopedia of Life Sciences 2001, John Wiley & Sons, Ltd. 10.1038/npg.els.0001408.
3. Walker I.D., Davidson J.F., Thomson J.M. Blood Coagulation and Hemostasis 1985, Churchill Livingstone, Edinburgh, 208-263.
4. Rajesh R., Natarajua A., Gowda C.D.R., Frey B.M., Frey F.J., Vishwanath B.S. Purification and characterization of a 34-kDa, heat stable glycoprotein from Synadenium grantii latex: action on human fibrinogen and fibrin clot. Biochimie 2006, 88:1313-1322.
5. Shivaprasad H.V., Rajesh R., Nanda B.L., Dharmappa K.K., Vishwanath B.S. Thrombin like activity of Asclepias curassavica L. latex: action of cysteine proteases. J.Ethnopharmacol. 2009, 123:106-109.
6. Shivaprasad H.V., Rajaiah R., Frey B.M., Frey F.J., Vishwanath B.S. 'Pergularaine I'- a plant cysteine protease with thrombin-like activity from Pergularia extensa latex. Thrombos. Res. 2010, 125:e100-e105.
7. Deng Z., Wang S., Li Q., Ji X., Zhang L., Hong M. Purification and characterization of a novel fibrinolytic enzyme from the polychaete, Neanthes japonica (Iznka). Bioresour. Technol. 2010, 101:1954-1960.
8. Wang S., Deng Z., Li Q., Ge X., Bo Q., Liu J., Cui J., Jiang X., Liu J., Zhang L., Hong M. Anovel alkaline serine protease with fibrinolytic activity from the polychaete, Neanthes japonica. Comp. Biochem. Physiol. Part B. 2011, 159:18-25.
9. Hahn B.S., Cho S.Y., Ahn M.Y., Kim Y.S. Purification and characterization of a plasmin-like protease from Tenodera sinensis (Chinese mantis). Insect Biochem. Mol. Biol. 2001, 3:573-581.
10. Chiou S., Hung C., Huang K. Characterization of a protease with α- and β-fibrinogenase activity from the western diamondback rattlesnake, Crotalus atrox. Biochem. Biophys. Res. Commun. 1992, 187:389-396.
11. Swenson S., Markland F.S. Snake venom fibrin(ogen)olytic enzymes. Toxicon 2005, 45:1021-1039.
12. Deepak V., Kalishwaralal K., Ramkumarpandian S., Babu S.V., Senthikumar S.R., Sangiliyandi G. Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour. Technol. 2008, 99:8170-8174.
13. Choi D., Cha W.S., Park N., Kimb H.W., Lee J.H., Park J.S., Park S.S. Purification and characterization of a novel fibrinolytic enzyme from fruiting bodies of Korean Cordyceps militaris. Bioresour. Technol. 2011, 102:3279-3285.
14. Fonseca K.C., Morais N.C.G., Queiroz M.R., Silva M.C., Gomes M.S., Costa J.O., Mamede C.C.N., Torres F.S., Silva N.P., Beletti M.E., Canabrava H.A.N., Oliveira F. Purification and biochemical characterization of Eumiliin from Euphorbia milii var. hislopii latex. Phytochemistry 2010, 71:708-715.
15. Caffini N.O., Lopez L.M.I., Natalucci C.L., Priolo N.S. Proteases of higher plants: General features, physiological roles and applications. Acta Farm. Bonaerense 1988, 7:195-213.
16. Lynn K.R., Clevette-Radford N.A. Proteases of Euphorbiaceae. Phytochemistry 1988, 27:45-50.
17. Kiritikar K.R., Basu D.J. Illustrated Indian Medicinal Plants 2000, 9:3031-3033. Satguru Publications, New Delhi. K.S. Mhaskar, E. Blatter, J.F. Caius (Eds.).
18. Patil S.B., Naikwade N.S., Magdum C.S. Review on phytochemistry and pharmacological aspects of Euphorbia hirta Linn. J.Pharmacol. Res. Health Care 2009, 1:113-133.
19. Lanhers M.C., Fleurentin J., Dorfman P., Mortier F., Pelt J.M. Analgesic, antipyretic and anti-inflammatory properties of Euphorbia hirta. Planta Med. 1991, 57:225-231.
20. Akinrinmade J.F., Oyeleye O.A. Antimicrobial efficacy and tissue reaction of Euphorbia hirta ethanolic extract on canine wounds. Afr. J. Biotechnol. 2010, 9:5028-5031.
21. Johnson P.B., Abdurahman E.M., Tiam E.A., Abdu-Aguye I., Hussaini I.M. Euphorbia hirta leaf extracts increase urine output and electrolytes in rats. J.Ethnopharmacol. 1999, 65:63-69.
22. Pande M., Dubey V.K., Yadav S.C., Jagannadham M.V. Anovel serine protease cryptolepain from Cryptolepis buchanani: purification and biochemical characterization. J.Agric. Food Chem. 2006, 54:10141-10150.
23. Yadav S.C., Pande M., Jagannadham M.V. Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii. Phytochemistry 2006, 67:1414-1426.
24. Ahmed I.A.M., Morishima I., Babiker E.E., Mori N. Dubiumin, a chymotrypsin-like serine protease from the seeds of Solanum dubium Fresen. Phytochemistry 2009, 70:483-491.
25. Antao C.M., Malcata F.X. Plant serine proteases: biochemical, physiological and molecular features. Plant Physiol. Biochem. 2005, 43:637-650.
26. Moro L.P., Murakami M.T., Cabral H., Vidotto A., Tajara E.H., Arni R.K., Juliano L., Bonilla-Rodriguez G.O. Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii. Protein Pept. Lett. 2008, 15:724-730.
27. Chang C.T., Fan M.H., Kuo F.C., Sung H.Y. Potent fibrinolytic enzyme from a mutant of Bacillus subtilis. J.Agric. Food Chem. 2000, 48:3210-3216.
28. Komori Y., Sugihara H., Tu A.T. Specificity of haemorrhagic proteinase from Crotalus atrox (Western diamond back rattle snake) venom. Biochim. Biophys. Acta 1985, 829:127-130.
29. Hung Chin-Chun, Chiou Shyh-Horng Fibrinogenolytic proteases isolated from the snake venom of Taiwan habu: serine proteases with kallikrein-like and angiotensin-degrading activities. Biochem. Biophys. Res. Commun. 2001, 281:1012-1018.
30. Arima K., Uchikoba T., Yonezawa H., Shimada M., Kaneda M. Cucumisin-like protease from the latex of Euphorbia supine. Phytochemistry 2000, 53:639-644.
31. Cui L., Dong M.S., Chen X.H., Ziang M., Lv X., Yan G. Anovel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom. World J. Micriobiol. Biotechnol. 2008, 24:483-489.
32. Kim J.S., Kumar S., Park S.E., Choi B.S., Kim S., Nguyen T.H., Kim C.S., Choi H.S., Kim M.K., Chun H.S., Park Y., Kim S.J. Afibrinolytic enzyme from the medicinal mushroom Cordyceps militaris. J.Microbiol. 2006, 44:622-631.
33. Bradford M.M. Arapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
34. Matsudaria P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J.Biol. Chem. 1987, 262:10035-10038.
35. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J.Mol. Biol. 1990, 215:403-410.
36. Choi Nack-Shick, Yoon Kab-Seog, Lee Jin-Young, Han Kyoung-Yoen, Kim Seung-Ho Comparison of three substrates (casein, fibrin, and gelatin) in zymographic gel. J.Biochem. Mol. Biol. 2001, 34:531-536.
37. Whitmore L., Wallace B.A. DICHROWEB: an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucl. Acids Res. 2004, 32:W668-W673.