Prestin in HEK cells is an obligate tetramer

Journal of Neurophysiology

Volumn 107, Issue 1, 2012, Pages 5-11

  Hallworth, Richard ^a   Nichols, Michael G ^b  

^a CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CREIGHTON UNIVERSITY   (United States)

Author keywords

Electromotility; Hair cell; Outer hair cell

Indexed keywords

CYCLIC NUCLEOTIDE GATED CHANNEL; ENHANCED GREEN FLUORESCENT PROTEIN; PRESTIN; TETRAMER;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; KIDNEY CELL; OLIGOMERIZATION; OSMOSIS; PLASMID; PRIORITY JOURNAL; PROTEIN SYNTHESIS; STOICHIOMETRY;

ANION TRANSPORT PROTEINS; CELL MEMBRANE; DIMERIZATION; HEK293 CELLS; HUMANS;

EID: 84255182677     PISSN: 00223077     EISSN: 15221598     Source Type: Journal    
DOI: 10.1152/jn.00728.2011     Document Type: Article

References (33)

1. Aitken CE, Marshall RA, Puglisi JD. An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J 94: 1826-1835, 2008.
2. Bonigk W, Altenhofen W, Muller F, Dose A, Illing M, Molday RS, Kaupp UB. Rod and cone photoreceptor cells express distinct genes for cGMP- gated channels. Neuron 10: 865-877, 1993.
3. de Monvel JB, Brownell WE, Ulfendahl M. Lateral diffusion anisotropy and membrane lipid/skeleton interaction in outer hair cells. Biophys J 91: 364-381, 2006.
4. Cheatham MA, Huynh KH, Gao J, Zuo J, Dallos P. Cochlear function in Prestin knockout mice. J Physiol 560: 821-830, 2004.
5. Currall BB, Rossino D, Jensen-Smith H, Hallworth RJ. The roles of conserved and non-conserved cysteinyl residues in the oligomerization and function of mammalian Prestin. J Neurophysiol; published ahead of print August 3, 2011, doi:10.1152/jn.00496.2011.
6. Dallos P, Wu X, Cheatham MA, Gao J, Zheng J, Anderson CT, Jia S, Wang X, Cheng WH, Sengupta S, He DZ, Zuo J. Prestin-based outer hair cell motility is necessary for mammalian cochlear amplification. Neuron 58: 333-339, 2008.
7. Deak L, Zheng J, Orem A, Du GG, Aguinaga S, Matsuda K, Dallos P. Effects of cyclic nucleotides on the function of prestin. J Physiol 563: 483-496, 2005.
8. Detro-Dassen S, Schanzler M, Lauks H, Martin I, zu Berstenhorst SM, Nothmann D, Torres-Salazar D, Hidalgo P, Schmalzing G, Fahlke C. Conserved dimeric subunit stoichiometry of SLC26 multifunctional anion exchangers. J Biol Chem 283: 4177-4188, 2008.
9. Gleitsman KR, Tateyama M, Kubo Y. Structural rearrangements of the motor protein prestin revealed by fluorescence resonance energy transfer. Am J Physiol Cell Physiol 297: C290-C298, 2009.
10. Greeson JN, Organ LE, Pereira FA, Raphael RM. Assessment of prestin self-association using fluorescence resonance energy transfer. Brain Res 1091: 140-150, 2006.
11. Harms GS, Cognet L, Lommerse PH, Blab GA, Kahr H, Gamsjager R, Spaink HP, Soldatov NM, Romanin C, Schmidt T. Single-molecule imaging of l-type Ca(2+) channels in live cells. Biophys J 81: 2639-2646, 2001.
12. Harms GS, Orr G, Montal M, Thrall BD, Colson SD, Lu HP. Probing conformational changes of gramicidin ion channels by single-molecule patch-clamp fluorescence microscopy. Biophys J 85: 1826-1838, 2003.
13. He DZ, Jia S, Sato T, Zuo J, Andrade LR, Riordan GP, Kachar B. Changes in plasma membrane structure and electromotile properties in prestin deficient outer hair cells. Cytoskeleton 67: 43-55, 2010.
14. Heine M, Groc L, Frischknecht R, Beique JC, Lounis B, Rumbaugh G, Huganir RL, Cognet L, Choquet D. Surface mobility of postsynaptic AMPARs tunes synaptic transmission. Science 320: 201-205, 2008.
15. Jacquier V, Prummer M, Segura JM, Pick H, Vogel H. Visualizing odorant receptor trafficking in living cells down to the single-molecule level. Proc Natl Acad Sci USA 103: 14325-14330, 2006.
16. Ji W, Xu P, Li Z, Lu J, Liu L, Zhan Y, Chen Y, Hille B, Xu T, Chen L. Functional stoichiometry of the unitary calcium-release-activated calcium channel. Proc Natl Acad Sci USA 105: 13668-13673, 2008.
17. Kalinec F, Holley MC, Iwasa KH, Lim DJ, Kachar B. A membrane-based force generation mechanism in auditory sensory cells. Proc Natl Acad Sci USA 89: 8671-8675, 1992.
18. Kaupp UB, Niidome T, Tanabe T, Terada S, Bonigk W, Stuhmer W, Cook NJ, Kangawa K, Matsuo H, Hirose T, Miyata T, Numa S. Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel. Nature 342: 762-766, 1989.
19. Koppl C, Forge A, Manley GA. Low density of membrane particles in auditory hair cells of lizards and birds suggests an absence of somatic motility. J Comp Neurol 479: 149-155, 2004.
20. Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature 443: 355-358, 2006.
21. Liberman MC, Gao J, He DZ, Wu X, Jia S, Zuo J. Prestin is required for electromotility of the outer hair cell and for the cochlear amplifier. Nature 419: 300-304, 2002.
22. Liu DT, Tibbs GR, Siegelbaum SA. Subunit stoichiometry of cyclic nucle-otide-gated channels and effects of subunit order on channel function. Neuron 16: 983-990, 1996.
23. Mio K, Kubo Y, Ogura T, Yamamoto T, Arisaka F, Sato C. The motor protein prestin is a bullet-shaped molecule with inner cavities. J Biol Chem 283: 1137-1145, 2008.
24. Murakoshi M, Gomi T, Iida K, Kumano S, Tsumoto K, Kumagai I, Ikeda K, Kobayashi T, Wada H. Imaging by atomic force microscopy of the plasma membrane of prestin-transfected Chinese hamster ovary cells. J Assoc Res Otolaryngol 7: 267-278, 2006.
25. Navaratnam D, Bai JP, Samaranayake H, Santos-Sacchi J. N-terminal-mediated homomultimerization of prestin, the outer hair cell motor protein. Biophys J 89: 3345-3352, 2005.
26. Rajagopalan L, Greeson JN, Xia A, Liu H, Sturm A, Raphael RM, Davidson AL, Oghalai JS, Pereira FA, Brownell WE. Tuning of the outer hair cell motor by membrane cholesterol. J Biol Chem 282: 36659-36670, 2007.
27. Santos-Sacchi J, Navarrete E. Voltage-dependent changes in specific membrane capacitance caused by prestin, the outer hair cell lateral membrane motor. Pflügers Arch 444: 99-106, 2002.
28. Tombola F, Ulbrich MH, Isacoff EY. The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor. Neuron 58: 546-556, 2008.
29. Ulbrich MH, Isacoff EY. Subunit counting in membrane-bound proteins. Nat Methods 4: 319-321, 2007.
30. Wang X, Yang S, Jia S, He DZ. Prestin forms oligomer with four mechanically independent subunits. Brain Res 1333: 28-35, 2010.
31. Wu X, Currall B, Yamashita T, Parker LL, Hallworth R, Zuo J. Prestin-prestin and prestin-GLUT5 interactions in HEK293T cells. Dev Neurobiol 67: 483-497, 2007.
32. Zheng J, Du GG, Anderson CT, Keller JP, Orem A, Dallos P, Cheatham M. Analysis of the oligomeric structure of the motor protein prestin. J Biol Chem 281: 19916-19924, 2006.
33. Ziegler U, Vinckier A, Kernen P, Zeisel D, Biber J, Semenza G, Murer H, Groscurth P. Preparation of basal cell membranes for scanning probe microscopy. FEBS Lett 436: 179-184, 1998.