Variable agronomic practices, cultivar, strain source and initial contamination dose differentially affect survival of Escherichia coli on spinach

Journal of Applied Microbiology

Volumn 112, Issue 1, 2012, Pages 109-118

  Gutierrez Rodriguez E ^a   Gundersen, A ^a   Sbodio, A O ^a   Suslow, T V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Escherichia coli O157:H7; Phyllosphere; Rhizosphere; Spinach; Strain source; Surrogate

Indexed keywords

AGRONOMY; PATHOGENS; PLANTS (BOTANY); RECOVERY; SOILS;

AGRONOMIC PRACTICES; E. COLI; E.COLI O157:H7; ESCHERICHIA COLI O157:H7; INOCULA; PHYLLOSPHERES; RHIZOSPHERE; SPINACH; STRAIN SOURCE; SURROGATE;

ESCHERICHIA COLI;

AGRONOMY; CULTIVAR; DISEASE CONTROL; FECAL COLIFORM; FITNESS; HARVESTING; HYDROPONICS; INOCULATION; LEAF; LEAFY VEGETABLE; MOISTURE CONTENT; PATHOGEN; PERSISTENCE; PHYLLOSPHERE; QUANTITATIVE ANALYSIS; RHIZOSPHERE; SHOOT; SURVIVAL; WATER AVAILABILITY;

AGRONOMIC TRAIT; ARTICLE; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIUM CONTAMINATION; COLONY FORMING UNIT; CULTIVAR; DEVELOPMENTAL STAGE; ESCHERICHIA COLI; ESCHERICHIA COLI O157; INOCULATION; MOISTURE; NONHUMAN; PLANT GROWTH; PLANT LEAF; QUANTITATIVE ANALYSIS; RHIZOSPHERE; SHOOT; SPINACH; SURFACE PROPERTY; WATER AVAILABILITY;

ESCHERICHIA COLI; SPINACIA OLERACEA;

EID: 83555179092     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05184.x     Document Type: Article

References (34)

1. Bearson, S.M., Benjamin, W.H., Swords, W.E. and Foster, J.W. (1996) Acid shock induction of RpoS is mediated by the mouse virulence gene mviA of Salmonella typhimurium. J Bacteriol 178, 2572-2579.
2. Beattie, G.A. and Lindow, S.E. (1999) Bacterial colonization of leaves: a spectrum of strategies. Am Phytopathol Soc 89, 353-359.
3. Beuchat, L.R., Ward, T.E. and Pettigrew, C.A. (2001) Comparison of chlorine and a prototype produce wash product for effectiveness in killing Salmonella and Escherichia coli O157: H7 on alfalfa seeds. J Food Prot 64, 152-158.
4. Brandl, M.T. (2006) Fitness of human enteric pathogens on plants and implications for food safety. Annu Rev Phytopathol 44, 367-392.
5. Brandl, M.T. and Amundson, R. (2008) Leaf age as a risk factor in contamination of lettuce with Escherichia coli O157:H7 and Salmonella enterica. Appl Environ Microbiol 74, 2298-2306.
6. Campbell, G.R., Prosser, J., Glover, A. and Killham, K. (2001) Detection of Escherichia coli O157:H7 in soil and water using multiplex PCR. J Appl Microbiol 91, 1004-1010.
7. Delaquis, P., Bach, S. and Dinu, L.D. (2007) Behavior of Escherichia coli O157:H7 in leafy vegetables. J Food Prot 70, 1966-1974.
8. Durso, L.M., Smith, D. and Hutkins, R.W. (2004) Measurements of fitness and competition in commensal Escherichia coli and E. coli O157:H7 strains. Appl Environ Microbiol 70, 1435-1443.
9. Erickson, M.C., Webb, C.C., Diaz-Perez, J.C., Phatak, S.C., Silvoy, J.J., Davey, L., Payton, A.S., Liao, J. et al. (2010a) Surface and internalized Escherichia coli O157:H7 on field grown spinach and lettuce treated with spray-contaminated irrigation water. J Food Prot 73, 1023-1029.
10. Erickson, M.C., Webb, C.C., Diaz-Perez, J.C., Phatak, S.C., Silvoy, J.J., Davey, L., Payton, A.S., Liao, J. et al. (2010b) Infrequent internalization of Escherichia coli O157:H7 into field grown leafy greens. J Food Prot 73, 500-506.
11. Gruda, N. (2005) Impact of environmental factors on product quality of greenhouse vegetables for fresh consumption. Crit Rev Plant Sci 24, 227-247.
12. Gutierrez, E. (2009) Influence of nitrogen on spinach leaf fragility, composition, and postharvest quality. MSc thesis at (accessed 10 November 2011).
13. Ibekwe, M.A., Grieve, C.M., Papiernik, S.K. and Yang, C.H. (2009) Persistence of Escherichia coli O157:H7 on the rhizosphere and phyllosphere of lettuce. Appl Microbiol 49, 784-790.
14. Koike, S., Cahn, M., Cantwell, M., Fennimore, S., LeStrange, M., Natwick, E., Smith, R.F. and Takele, E. (2011) Spinach Production in California. Oakland: University of California. ANR publication 7212.
15. Kolling, G.L. and Matthews, K.R. (2001) Examination of recovery in vitro and in vivo of nonculturable Escherichia coli O157:H7. Appl Environ Microbiol 67, 3928-3933.
16. Lang, N.L., Bellett-Travers, M.D. and Smith, S.R. (2007) Field investigations on the survival of Escherichia coli and presence of other enteric micro-organisms in biosolids-amended agricultural soil. J Appl Microbiol 103, 1868-1882.
17. Liu, Y., Wang, C., Tyrell, G. and Li, X.F. (2010) Production of Shiga-like toxins in viable but nonculturable Escherichia coli O157:H7. Water Res 44, 711-718.
18. Mitra, R., Cuesta-Alonso, E., Wayadande, A., Talley, J., Gilliand, S. and Fletcher, J. (2009) Effect of route of introduction and host cultivar on the colonization, internalization, and movement of the human pathogen Escherichia coli O157:H7 in spinach. J Food Prot 72, 1521-1530.
19. Moretro, T., Heir, E., Mo, K.R., Habimana, O., Abdelgani, A. and Langsrud, S. (2010) Factors affecting survival of shigatoxin-producing Escherichia coli on abiotic surfaces. Intern J Food Microbiol 138, 71-77.
20. Moyne, A.L., Sudarshana, M.R., Blessington, T., Koike, S.T., Cahn, M.D. and Harris, L. (2011) Fate of Escherichia coli O157:H7 in field-inoculated lettuce. Food Microbiol 28, 1417-1425.
21. Muffler, A., Traulsen, D.D., Lange, R. and Hengge-Aronis, R. (1996) Post-transcriptional regulation of the sigma S subunit of RNA polymerase in Escherichia coli. J Bacteriol 178, 1607-1613.
22. Neumann, G. and Römheld, V. (2000) The release of root exudates as affected by the plant physiological status. In The Rhizosphere: Biochemistry and Organic Substances at the Soil-Plant Interface ed. Pinton, R., Varanini, Z. and Nannipieri, Z. pp. 41-93. New York, NY: CRC Press.
23. Oliver, J.D. (2005) The viable but non-culturable state in bacteria. J Microbiol 43, 93-100.
24. Patel, J., Millner, P., Nou, X. and Sharma, M. (2009) Persistence of enterohaemorrhagic and nonpathogenic E. coli on spinach leaves and in the rhizosphere soil. J Appl Microbiol 108, 1789-1796.
25. Pu, S., Beaulieu, J.C., Prinyawiwatkul, W. and Ge, B. (2009) Effects of plant maturity and growth media bacterial inoculum level on the surface contamination and internalization of Escherichia coli O157:H7 in growing spinach leaves. J Food Prot 72, 2313-2320.
26. Sharma, M., Ingram, D.T., Patel, J.T., Millner, P.D., Wang, Z., Hull, A.E. and Donneberg, M.S. (2009) A novel approach to investigate the uptake and internalization of Escherichia coli O157:H7 in spinach cultivated in Soil and hydroponic medium. J Food Prot 72, 1513-1520.
27. Teplitski, M., Barak, J.D. and Schneider, K.R. (2010) Human enteric pathogen in produce: un-answered ecological questions with direct implications for food safety. Curr Opin Biotechnol 20, 166-171.
28. Touchon, M., Hoede, C., Tenaillon, O., Barbe, V., Baeriswyl, S. and Bidet, P. (2009) Organized genome dynamics in the Escherichia coli species results in highly diverse adaptive paths. PLoS Genet 5, e1000344.
29. Van Elsas, J.D., Semenov, A.V., Costa, R. and Trevors, J.T. (2011) Survival of Escherichia coli in the environment: fundamental and public health aspects. ISME: Multidiscip J Microbiol Ecol 5, 173-183.
30. Vital, M., Hammes, F. and Egli, T. (2008) Escherichia coli O157 can grow in natural freshwater at low carbon concentrations. Environ Microbiol 10, 2387-2396.
31. Wilson, M. and Lindow, S.E. (1994) Inoculum density-dependent mortality and colonization of the phyllosphere by Pseudomonas syringae. Appl Environ Microbiol 60, 2232-2237.
32. Wilson, M., Hirano, S. and Lindow, S.E. (1999) Location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf. Appl Environ Microbiol 65, 1435-1443.
33. Winfield, M.D. and Groisman, E.A. (2003) Role of nonhost environments in the life style of Salmonella and Escherichia coli. Appl Environ Microbiol 69, 3687-3694.
34. Wood, J.D., Bezanson, G.S., Gordon, R.J. and Jamieson, R. (2010) Population dynamics of Escherichia coli inoculated by irrigation into the phyllosphere of spinach grown under commercial production conditions. Inter J Food Microbiol 143, 198-2004.