A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts

International Endodontic Journal

Volumn 45, Issue 1, 2012, Pages 12-18

  Scelza, M Z ^a   Linhares, A B ^a   Da Silva, L E ^a   Granjeiro, J M ^a,b   Alves, G G ^a  

^a FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

^b INSTITUTO NACIONAL DE METROLOGIA   (Brazil)

Author keywords

Biocompatibility; Endodontic sealer; Human primary osteoblasts; Multiparametric assay

Indexed keywords

2,3 BIS(2 METHOXY 4 NITRO 5 SULFOPHENYL) 5 ((PHENYLAMINO)CARBONYL) 2H TETRAZOLIUM HYDROXIDE; 2,3-BIS(2-METHOXY-4-NITRO-5-SULFOPHENYL)-5-((PHENYLAMINO)CARBONYL)-2H-TETRAZOLIUM HYDROXIDE; ALUMINUM DERIVATIVE; BIOMATERIAL; CALCIUM DERIVATIVE; CALCIUM HYDROXIDE; COLORING AGENT; CRYSTAL VIOLET; DIAGNOSTIC AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; MINERAL TRIOXIDE AGGREGATE; NEUTRAL RED; OXIDE; REAL SEAL COMPOSITE RESIN; RESIN; ROOT CANAL FILLING MATERIAL; SALICYLIC ACID DERIVATIVE; SEALAPEX; SILICATE; TETRAZOLIUM; ZINC OXIDE EUGENOL;

ARTICLE; CELL COUNT; CELL MEMBRANE; CELL SURVIVAL; COMPARATIVE STUDY; CULTURE MEDIUM; DRUG COMBINATION; DRUG EFFECT; ENZYMOLOGY; HUMAN; MATERIALS TESTING; MITOCHONDRION; OSTEOBLAST; TEMPERATURE; TIME;

ALUMINUM COMPOUNDS; BIOCOMPATIBLE MATERIALS; CALCIUM COMPOUNDS; CALCIUM HYDROXIDE; CELL COUNT; CELL MEMBRANE; CELL SURVIVAL; COLORING AGENTS; COMPOSITE RESINS; CULTURE MEDIA; DRUG COMBINATIONS; GENTIAN VIOLET; HUMANS; INDICATORS AND REAGENTS; MATERIALS TESTING; MITOCHONDRIA; NEUTRAL RED; OSTEOBLASTS; OXIDES; ROOT CANAL FILLING MATERIALS; SALICYLATES; SILICATES; TEMPERATURE; TETRAZOLIUM SALTS; TIME FACTORS; ZINC OXIDE-EUGENOL CEMENT;

EID: 83555164787     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/j.1365-2591.2011.01941.x     Document Type: Article

References (30)

1. Al-Hiyasat AS, Tayyar M, Darmani H (2010) Cytotoxicity evaluation of various resin based root canal sealers. International Endodontic Journal 43, 148-53.
2. ASTM International (2007) International Standard practice for direct contact cell culture evaluation of materials for medical devices. ASTM Standard Test Method F 0813-07 ed.
3. Bernath M, Szabo J (2003) Tissue reaction initiated by different sealers. International Endodontic Journal 36, 256-61.
4. Berridge MV, Herst PM, Tan AS (2005) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Reviews 11, 127-52.
5. Bouillaguet S, Wataha JC, Lockwood PE, Galgano C, Golay A, Krejci I (2004) Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. European Journal of Oral Sciences 112, 182-7.
6. Bouillaguet S, Wataha JC, Tay FR, Brackett MG, Lockwood PE (2006) Initial in vitro biological response to contemporary endodontic sealers. Journal of Endodontics 32, 989-92.
7. Brackett MG, Marshall A, Lockwood PE et al. (2008) Cytotoxicity of endodontic materials over 6-weeks ex vivo. International Endodontic Journal 41, 1072-8.
8. Brackett MG, Messer RL, Lockwood PE et al. (2010) Cytotoxic response of three cell lines exposed in vitro to dental endodontic sealers. Journal of Biomedical Materials Research Part B Applied Biomaterials 95, 380-6.
9. Bryan TE, Khechen K, Brackett MG et al. (2010) In vitro osteogenic potential of an experimental calcium silicate-based root canal sealer. Journal of Endodontics 36, 1163-9.
10. De-Deus G, Canabarro A, Alves G, Linhares A, Senne MI, Granjeiro JM (2009) Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells. Journal of Endodontics 35, 1387-90.
11. Eldeniz AU, Erdemir A, Kurtoglu F, Esener T (2007) Evaluation of pH and calcium ion release of Acroseal sealer in comparison with Apexit and Sealapex sealers. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontology 103, e86-e91.
12. Geurtsen W (2001) Biocompatibility of root canal filling materials. Australian Endodontic Journal 27, 12-21.
13. Gomes-Filho JE, Watanabe S, Bernabe PF, de Moraes Costa MT (2009a) A mineral trioxide aggregate sealer stimulated mineralization. Journal of Endodontics 35, 256-60.
14. Gomes-Filho JE, Watanabe S, Gomes AC, Faria MD, Lodi CS, Penha Oliveira SH (2009b) Evaluation of the effects of endodontic materials on fibroblast viability and cytokine production. Journal of Endodontics 35, 1577-9.
15. Gorduysus M, Avcu N, Gorduysus O et al. (2007) Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts. Journal of Endodontics 33, 1450-4.
16. Haustveit G, Torheim B, Fystro D, Eidem T, Sandvik M (1984) Toxicity testing of medical device materials tested in human tissue cultures. Biomaterials 5, 75-80.
17. Huang FM, Chang YC (2002) Cytotoxicity of resin-based restorative materials on human pulp cell cultures. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontology 94, 361-5.
18. Huang TH, Ding SJ, Hsu TZ, Lee ZD, Kao CT (2004) Root canal sealers induce cytotoxicity and necrosis. Journal of Material Sciences Materials in Medicine 15, 767-71.
19. Husoy T, Syversen T, Jenssen J (1993) Comparisons of four in vitro cytotoxicity tests: the MTT assay, NR assay, uridine incorporation and protein measurements. Toxicology In Vitro 7, 149-54.
20. International Organization for Standardization (2007) ISO 10993-12: biological evaluation of medical devices - Part 12: sample preparation and reference materials. ISO 10993-12:2007 ed.
21. International Organization for Standardization (2009) ISO 10993-5: biological evaluation of medical devices - Part 5: tests for in vitro cytotoxicity. ISO 10993-5:2009 ed.
22. ISO 7405:2008 (2008) Dentistry - preclinical evaluation of biocompatibility of medical devices used in dentistry - test methods for dental materials. ISO 7405:2008 ed.
23. Kim YK, Grandini S, Ames JM et al. (2010) Critical review on methacrylate resin-based root canal sealers. Journal of Endodontics 36, 383-99.
24. Leonardo RT, Consolaro A, Carlos IZ, Leonardo MR (2000) Evaluation of cell culture cytotoxicity of five root canal sealers. Journal of Endodontics 26, 328-30.
25. Putnam KP, Bombick DW, Doolittle DJ (2002) Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicology In Vitro 16, 599-607.
26. Scotti R, Tiozzo R, Parisi C, Croce MA, Baldissara P (2008) Biocompatibility of various root canal filling materials ex vivo. International Endodontic Journal 41, 651-7.
27. Scudiero DA, Shoemaker RH, Paull KD et al. (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Research 48, 4827-33.
28. Vajrabhaya LO, Korsuwannawong S, Jantarat J, Korre S (2006) Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontology 102, e48-e50.
29. Xu P, Liang J, Dong G, Zheng L, Ye L (2010) Cytotoxicity of RealSeal on human osteoblast-like MG63 cells. Journal of Endodontics 36, 40-4.
30. Zhu Q, Haglund R, Safavi KE, Spangberg LS (2000) Adhesion of human osteoblasts on root-end filling materials. Journal of Endodontics 26, 404-6.