Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy

European Journal of Oral Sciences

Volumn 112, Issue 2, 2004, Pages 182-187

  Bouillaguet, Serge ^a,c   Wataha, John C ^b   Lockwood, Petra E ^b   Galgano, Camillo ^a   Golay, Antoine ^a   Krejci, Ivo ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

^c Section of Dental Medicine   (Switzerland)

Author keywords

Confocal microscopy; Cytotoxicity; Endodontic sealers; Sealing

Indexed keywords

ACRYLIC ACID; FISSURE SEALANT; LIPOPOLYSACCHARIDE; RHODAMINE; SILICON; SUCCINATE DEHYDROGENASE; CARBAMIC ACID DERIVATIVE; COLORING AGENT; DIAGNOSTIC AGENT; ENDOREZ; EPOXY RESIN; FLUORESCENT DYE; POLY(CARBAMOYL)SULFONATE; POLYESTER; RESIN; ROEKO SEAL; ROOT CANAL FILLING MATERIAL; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TOOTH CEMENT; TOPSEAL;

ANIMAL TISSUE; ARTICLE; CONFOCAL LASER MICROSCOPY; CYTOTOXICITY; CYTOTOXICITY TEST; ENDODONTICS; HUMAN; HUMAN TISSUE; NONHUMAN; TIME; TOOTH ROOT; TOOTH ROOT CANAL; ANALYSIS OF VARIANCE; ANIMAL; CELL STRAIN 3T3; CLASSIFICATION; CONFOCAL MICROSCOPY; DENTAL BONDING; DRUG EFFECT; MATERIALS TESTING; MOUSE; SURFACE PROPERTY; TOOTH DISEASE;

ANALYSIS OF VARIANCE; ANIMALS; BALB 3T3 CELLS; CARBAMATES; COLORING AGENTS; COMPOSITE RESINS; DENTAL BONDING; DENTAL CEMENTS; DENTAL LEAKAGE; EPOXY RESINS; FLUORESCENT DYES; HUMANS; LIPOPOLYSACCHARIDES; MATERIALS TESTING; MICE; MICROSCOPY, CONFOCAL; POLYESTERS; RHODAMINES; ROOT CANAL FILLING MATERIALS; ROOT CANAL OBTURATION; SUCCINATE DEHYDROGENASE; SURFACE PROPERTIES; TETRAZOLIUM SALTS; THIAZOLES; TIME FACTORS;

EID: 2342584644     PISSN: 09098836     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0722.2004.00115.x     Document Type: Article

References (31)

1. SCHILDER H. Filling root canal in three dimensions. Dent Clin North Am 1967; 11: 723-744.
2. SAUNDERS WP, SAUNDERS EM. Coronal leakage as a cause of failure in root-canal therapy: a review. Endod Dent Traumatol 1994; 10: 105-108.
3. BERNATH M, SZABO J. Tissue reaction initiated by different sealers. Int Endod J 2003; 36: 256-261.
4. SCHMALZ G, HOFFMANN M, WEIS K, SCHWEIKL H. Influence of albumin and collagen on the cell mortality evoked by zinc oxide-eugenol in vitro. J Endodont 2000; 26: 284-287.
5. HUME WR. The pharmacologic and toxicological properties of zinc oxide-eugenol. J Am Dent Assoc 1986; 113: 789-791.
6. HUANG FM, TAI KW, CHOU MY, CHANG YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002; 35: 153-158.
7. HOVLAND EJ, DUMSHA TC. Leakage evaluation in vitro of the root canal sealer cement Sealapex. Int Endod J 1985; 18: 179-182.
8. WILLERSHAUSEN B, MARROQUIN BB, SCHAFER D, SCHULZE R. Cytotoxicity of root canal filling materials to three different human cell lines. J Endodont 2000; 26: 703-707.
9. SCHWEIKL H, SCHMALZ G, FEDERLIN M. Mutagenicity of the root canal sealer AHPlus in the Ames test. Clin Oral Invest 1998; 2: 125-129.
10. HUANG TH, YANG JJ, LI H, KAO CT. The biocompatibility evaluation of epoxy resin-based root canal sealers in vitro. Biomaterials 2002; 23: 77-83.
11. HUUMONEN S, LENANDER-LUMIKARI M, SIGURDSSON A, ORSTAVIK D. Healing of apical periodontitis after endodontic treatment: a comparison between a silicone-based and a zinc oxide-eugenol-based sealer. Int Endod J 2003; 36: 296-301.
12. WU MK, TIGOS E, WESSELINK PR. An 18-month longitudinal study on a new silicon-based sealer, RSA RoekoSeal: a leakage study in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 94: 499-505.
13. WU MK, DE GEE AJ, WESSELINK PR, MOORER WR. Fluid transport and bacterial penetration along root canal fillings. Int Endod J 1993; 26: 203-208.
14. CANALDA-SAHLI C, BRAU-AGUADE E, SENTIS-VILALTA J, AGUADEBRUIX S. The apical seal of root canal sealing cements using a radionuclide detection technique. Int Endod J 1992; 25: 250-260.
15. HILTON TJ. Can modern restorative procedures and materials reliably seal cavities? In vitro investigations. Part 2. Am J Dent 2002; 15: 279-289.
16. BARTHEL CR, MOSHONOV J, SHUPING G, ORSTAVIK D. Bacterial leakage versus dye leakage in obturated root canals. Int Endod J 1999; 32: 370-375.
17. WU M-K, WESSELINK PR. Endodontic leakage studies reconsidered. Part 1. Methodology, application and relevance. Int Endod J 1993; 26: 37-43.
18. CHAVEZ DE PAZ LE, DAHLEN G, MOLANDER A, MOLLER A, BERGENHOLTZ G. Bacteria recovered from teeth with apical periodontitis after antimicrobial endodontic treatment. Int Endod J 2003; 36: 500-508.
19. STASHENKO P, TELES R, D'SOUZA R. Periapical inflammatory responses and their modulation. Crit Rev Oral Biol Medical 1998; 9: 498-521.
20. LAGHIOS CD, CUTLER CW, GUTMANN JL. In vitro evidence that lipopolysaccharide of an oral pathogen leaks from root-end filled teeth. Int Endod J 2000; 33: 333-339.
21. COLEMAN AJ. Macromolecular leakage beneath full cast crowns. Part I. The diffusion of lipopolysaccharide and dextran. J Prosthet Dent 1995; 74: 187-189.
22. HANKS CT, WATAHA JC, SUN Z. In vitro models of biocompatibility: a review. Dent Mater 1996; 12: 186-193.
23. WATAHA JC, CRAIG RG, HANKS CT. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater 1992; 8: 65-70.
24. WATSON TF, AZZOPARDI A, ETMAN M, CHENG PC, SIDHU SK. Confocal and multi-photon microscopy of dental hard tissues and biomaterials. Am J Dent, 2000: 13(Spec No): 19D-24D.
25. NELSON SK, WATAHA JC, NEME AM, CIBIRKA RM, LOCKWOOD PE. Cytotoxicity of dental casting alloys pretreated with biologic solutions. J Prosthet Dent 1999; 81: 591-596.
26. INTERNATIONAL STANDARDS ORGANIZATION. Biological evaluation of medical devices, Part 5: Tests for cytotoxicity: in vitro models. ISO 10993-5, 1st edition, 1992-12-15. Geneva: ISO, 1997.
27. BRATEL J, JONTELL M, DAHLGREN U, BERGENHOLTZ G. Effects of root canal sealers on immunocompetent cells in vitro and in vivo. Int Endod J 1998; 31: 178-188.
28. SCHWARZE T, LEYHAUSEN G, GEURTSEN W. Long-term cytocompatibility of various endodontic sealers using a new root canal model. J Endodont 2002; 28: 749-753.
29. WATSON TF. Fact and artefact in confocal microscopy. Adv Dent Res 1997; 11: 433-441.
30. COBANKARA FK, ADANIR N, BELLI S, PASHLEY DH. A quantitative evaluation of apical leakage of four root-canal sealers. Int Endod J 2002; 35: 979-984.
31. MJOR IA, SMITH MR, FERRARI M, MANNOCCI F. The structure of dentine in the apical region of human teeth. Int Endod J 2001; 34: 346-353.