Establishment of Epstein-Barr virus growth-transformed lymphoblastoid cell lines

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Hui Yuen, Joyce ^a,b   McAllister, Shane ^a,b   Koganti, Siva ^b   Hill, Erik ^b   Bhaduri Mcintosh, Sumita ^a,b  

^a Renaissance School of Medicine at Stony Brook University   (United States)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

EBV; Epstein barr virus; Immortalization; Immunology; Issue 57; LCL; Lymphoblastoid cell lines; PBMC; Transformation

Indexed keywords

EID: 83455225438     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3321     Document Type: Article

References (15)

1. Thorley-Lawson, D.A. & Gross, A. Persistence of the Epstein-Barr virus and the origins of associated lymphomas. N. Engl. J. Med. 350, 1328-37 (2004).
2. Kubuschok, B., et al. Use of spontaneous Epstein-Barr virus lymphoblastoid cell lines genetically modified to express tumor antigen as cancer vaccines: mutated p21 ras oncogene in pancreatic carcinoma as a model. Hum. Gene Ther. 13, 815-27 (2002).
3. Kuppers, R.B cells under influence: transformation of B cells by Epstein-Barr virus. Nat. Rev. Immunol. 3, 801-12 (2003).
4. Traggiai, E., et al. An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus. Nat. Med. 10, 871-5 (2004).
5. Bernasconi, N., Traggiai, E., & Lanzavecchia, A. Maintenance of serological memory by polyclonal activation of human memory B cells. Science. 298, 2199-202 (2002).
6. Oh, H.-M., et al. An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytes. Cell Prolif. 36, 191-7 (2003).
7. Ventura, M., et al. Use of a simple method for the Epstein-Barr virus transformation of lymphocytes from members of large families of Reunion Island. Hum. Hered. 38, 36-43 (1988).
8. Henderson, E., et al. Efficiency of transformation of lymphocytes by Epstein-Barr virus. Virology. 76, 152-63 (1977).
9. Bird, A.G., et al. Characteristics of Epstein-Barr virus activation of human B lymphocytes. J. Exp. Med. 154, 832-9 (1981).
10. Neitzel, H. A routine method for the establishment of permanent growing lymphoblastoid cell lines. Hum. Genet. 73, 320-6 (1986).
11. Pelloquin, F., Lamelin, J.P., & Lenoir, G.M. Human B lymphocytes immortalization by Epstein-Barr virus in the presence of cyclosporin A. In Vitro Cell Dev. Biol. 22, 689-94 (1986).
12. Pressman, S. & Rotter, J.I. Epstein-Barr virus transformation of cryopreserved lymphocytes: prolonged experience with technique. Am. J. Hum. Genet. 49, 467 (1991).
13. Miller, G., et al. Epstein-Barr virus: Transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Nat. Acad. Sci. 69, 383-7 (1972).
14. Megyola, C., Ye, J., & Bhaduri-McIntosh, S. Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus. Virol. J. 8, 84 (2011).
15. Tosato, G. & Cohen, J. Generation of Epstein-Barr virus (EBV)-immortalized B cell lines, In Current Protocols of Immunology. Chapter 7.22 (1991).