A quantitative assay for insulin-expressing colony-forming progenitors

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Winkler, Michael ^a   Trieu, Nancy ^b   Feng, Tao ^b   Jin, Liang ^b   Walker, Stephanie ^b   Singh, Lipi ^b   Ku, Hsun Teresa ^b  

^a CALIFORNIA STATE UNIVERSITY CHANNEL ISLANDS   (United States)

^b Beckman Research Institute of City of Hope   (United States)

Author keywords

3 Dimensional culture; Colony assay; Developmental biology; Embryonic stem cells; Insulin expressing cells; Issue 57; Matrigel; Methylcellulose; Pancreas; Progenitor cells; Semi solid media

Indexed keywords

EID: 83455195357     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3148     Document Type: Article

References (16)

1. Ku, H.T., et al. Committing embryonic stem cells to early endocrine pancreas in vitro. Stem cells. 22, 1205-1217 (2004).
2. Ku, H.T., et al. Insulin-expressing colonies developed from murine embryonic stem cell-derived progenitors. Diabetes. 56, 921-929 (2007).
3. Chen, C., et al. Characterization of an in vitro differentiation assay for pancreatic-like cell development from murine embryonic stem cells: detailed gene expression analysis. Assay. Drug. Dev. Technol. 9, 403-419 (2011).
4. Lee, C.S., Perreault, N., Brestelli, J.E., & Kaestner, K.H. Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity. Genes & development. 16, 1488-1497 (2002).
5. Gu, G., Dubauskaite, J., & Melton, D.A. Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development 129, 2447-2457 (2002).
6. Ogawa, M. Differentiation and proliferation of hematopoietic stem cells. Blood. 81, 2844-2253. (1993).
7. Gittes, G.K., Galante, P.E., Hanahan, D., Rutter, W.J., & Debase, H.T. Lineage-specific morphogenesis in the developing pancreas: role of mesenchymal factors. Development. 122, 439-447. (1996).
8. Miralles, F., Battelino, T., Czernichow, P., & Scharfmann, R. TGF-beta plays a key role in morphogenesis of the pancreatic islets of Langerhans by controlling the activity of the matrix metalloproteinase MMP-2. J. Cell. Biol. 143, 827-836 (1998).
9. Bonner-Weir, S., et al. In vitro cultivation of human islets from expanded ductal tissue. Proc. Natl. Acad. Sci. U. S. A. 97, 7999-8004 (2000).
10. Gao, R., et al. Characterization of endocrine progenitor cells and critical factors for their differentiation in human adult pancreatic cell culture. Diabetes. 52, 2007-2015 (2003).
11. Gao, R., Ustinov, J., Korsgren, O., & Otonkoski, T. In vitro neogenesis of human islets reflects the plasticity of differentiated human pancreatic cells. Diabetologia. 48, 2296-2304 (2005).
12. Suarez-Pinzon, W.L., Lakey, J.R., Brand, S.J., & Rabinovitch, A. Combination therapy with epidermal growth factor and gastrin induces neogenesis of human islet beta-cells from pancreatic duct cells and an increase in functional beta-cell mass. J. Clin. Endocrinol. Metab. 90, 3401-3409 (2005).
13. Hao, E., et al. Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas. Nature Medicine. 12, 310-316 (2006).
14. Ku, H.T. Minireview: pancreatic progenitor cells--recent studies. Endocrinology. 149, 4312-4316 (2008).
15. Seaberg, R.M., et al. Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages. Nature Biotechnology. 22, 1115-1124 (2004).
16. Rovira, M., et al. Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas. Proc. Natl. Acad. Sci. U. S. A. 107, 75-80 (2010).