A monomeric mutant of restriction endonuclease EcoRI nicks DNA without sequence specificity

Biological Chemistry

Volumn 385, Issue 10, 2004, Pages 975-985

  Fritsche, Petra ^a   Alves, Jürgen ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Heterodimer; Nuclease; Protein engineering; Protein nucleic acid interaction; Restriction enzyme; Sequence specificity

Indexed keywords

ASPARTIC ACID; DIMER; DNA; DOUBLE STRANDED DNA; ISOLEUCINE; LEUCINE; LYSINE; MONOMER; NUCLEOTIDE; SINGLE STRANDED DNA; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ALPHA HELIX; ARTICLE; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DIMERIZATION; DNA BINDING; DNA DEGRADATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STABILITY; ENZYME SUBUNIT; HYDROPHOBICITY; MOLECULAR INTERACTION; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REPRODUCIBILITY; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; WILD TYPE;

DEOXYRIBONUCLEASE ECORI; DNA MUTATIONAL ANALYSIS; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; DNA-BINDING PROTEINS; MUTATION; PROTEIN CONFORMATION; SEQUENCE ANALYSIS, DNA;

EID: 8344234963     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2004.127     Document Type: Article

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