Measuring dissociation rate constants of protein complexes through subunit exchange: Experimental design and theoretical modeling

PLoS ONE

Volumn 6, Issue 12, 2011, Pages

  Pan, Chongle ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HETERODIMER; MULTIPROTEIN COMPLEX;

ANALYTIC METHOD; ARTICLE; CELL LYSATE; COMPLEX FORMATION; DISSOCIATION CONSTANT; EXPERIMENTAL DESIGN; HOMOLOGOUS RECOMBINATION; ISOTOPE LABELING; MASS SPECTROMETRY; MATHEMATICAL COMPUTING; MATHEMATICAL MODEL; METABOLICALLY LABELED AFFINITY TAGGED SUBUNIT EXCHANGE METHOD; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN INTERACTION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; VALIDATION PROCESS; BIOLOGICAL MODEL; KINETICS; METABOLISM; METHODOLOGY; PROTEIN SUBUNIT; STAINING;

KINETICS; MODELS, BIOLOGICAL; MULTIPROTEIN COMPLEXES; PROTEIN SUBUNITS; RESEARCH DESIGN; STAINING AND LABELING;

EID: 83355172872     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0028827     Document Type: Article

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