Axenic culture and identification of amastigotes from Sichuan human strain of Chinese Leishmania isolates

Veterinary Parasitology

Volumn 183, Issue 3-4, 2012, Pages 353-355

  Cao, De Ping ^a   Chen, Da Li ^a   Chen, Jian Ping ^a   Liao, Lin ^a   Yang, Bin Bin ^a   Hu, Xiao Su ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Amastigote; Chinese Leishmania isolate; In vitro; Promastigote; Transformation

Indexed keywords

HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G; POLYCLONAL ANTIBODY;

AMASTIGOTE; ARTICLE; AXENIC CULTURE; CHINA; CHINESE; CONTROLLED STUDY; DEVELOPMENTAL STAGE; GENE; GP46 GENE; LEISHMANIA; MICROMORPHOLOGY; NONHUMAN; P4 GENE; PARASITE IDENTIFICATION; PARASITE ISOLATION; PROMASTIGOTE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRASTRUCTURE;

AXENIC CULTURE; CHINA; HUMANS; IMMUNOBLOTTING; LEISHMANIA; PARASITOLOGY; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 83255185148     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2011.07.044     Document Type: Article

References (14)

1. Alcolea P.J., Alonso A., Gómez M.J., Sánchez-Gorostiaga A., Moreno-Paz M., González-Pastor E., Toraño A., Parro V., Larraga V. Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum. BMC Genom. 2010, 11:31.
2. Bates P.A. Axenic culture of Leishmania amastigotes. Parasitol. Today 1993, 9:143-146.
3. Cao D.-P., Guo X.-G., Chen D.-L., Chen J.-P. Species delimitation and phylogenetic relationships of Chinese Leishmania isolates re-examined using kinetoplast cytochrome oxidase II gene sequences. Parasitol. Res. 2011, 190:163-173.
4. Debrabant A., Joshi M.B., Pimenta P.F., Dwyer D.M. Generation of Leishmania donovani axenic amastigotes: their growth and biological characteristics. Int. J. Parasitol. 2004, 34:205-217.
5. Duncan R., Alvarez R., Jaffe C.L., Wiese M., Klutch M., Shakarian A., Dwyer D., Nakhasi H.L. Early response gene expression during differentiation of cultured Leishmania donovani. Parasitol. Res. 2001, 87:897-906.
6. Gupta N., Goyal N., Rastogi A.K. In vitro cultivation and characterization of axenic amastigotes of Leishmania. Trends Parasitol. 2001, 17:150-153.
7. Hodgkinson V.H., Soong L., Duboise S.M., McMahon-Pratt D. Leishmania amazonensis: cultivation and characterization of axenic amastigote-like organisms. Exp. Parasitol. 1996, 83:94-105.
8. Pan A.A., Duboise S.M., Eperon S., Rivas L., Hodgkinson V., Traub-Cseko Y., McMahon-Pratt D. Developmental life cycle of Leishmania cultivation and characterization of cultured extracellular amastigotes. J. Eukaryot. Microbiol. 1993, 40:213-223.
9. Pan A.A. Leishmania mexicana: serial cultivation of intracellular stages in a cell-free medium. Exp. Parasitol. 1984, 58:72-80.
10. Sacks D.L. The structure and function of the surface lipophosphoglycan on different developmental stages of Leishmania promastigotes. Infect. Agents Dis. 1992, 1:200-206.
11. Teixeira M.C., de Jesus Santos R., Sampaio R.B., Pontes-de-Carvalho L., dos-Santos W.L. A simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania promastigotes species. Parasitol. Res. 2002, 88:963-968.
12. Wang H.-M., Lynn S. Detection of P-4 and GP-46 expression in Leishmania amazonensis amastigotes and promastigotes by RT-PCR. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2006, 24:266-268. (in Chinese with English abstract).
13. Yang B.-B., Guo X.-G., Hu X.-S., Zhang J.-G., Liao L., Chen D.-L., Chen J.-P. Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences. Parasitol. Res. 2010, 107:1049-1065.
14. Zilberstein D., Shapira M. The role of pH and temperature in the development of Leishmania parasites. Annu. Rev. Microbiol. 1994, 48:449-470.