Development of cathepsin-L cysteine proteinase based Dot-enzyme-linked immunosorbent assay for the diagnosis of Fasciola gigantica infection in buffaloes

Veterinary Parasitology

Volumn 183, Issue 3-4, 2012, Pages 382-385

  Varghese, Anju ^a   Raina, O K ^a   Nagar, Gaurav ^a   Garg, Rajat ^a   Banerjee, P S ^a   Maharana, B R ^a   Kollannur, Justin D ^a  

^a INDIAN VETERINARY RESEARCH INSTITUTE   (India)

Author keywords

Buffalo; Cathepsin L cysteine proteinase; Dot ELISA; Fasciola gigantica; Immunodiagnosis

Indexed keywords

CATHEPSIN L; IMMUNOGLOBULIN G ANTIBODY;

ANTIGEN BINDING; ARTICLE; AUTOPSY; BUFFALO; CONTROLLED STUDY; CROSS REACTION; DOT ENZYME LINKED IMMUNOSORBENT ASSAY; ECHINOCOCCOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PURIFICATION; FASCIOLA GIGANTICA; FASCIOLIASIS; FECES ANALYSIS; GASTROTHYLAX; GIGANTOCOTYLE EXPLANATUM; HELMINTH; HOST; INTESTINE; LIVER; MICROSCOPY; NONHUMAN; PARAMPHISTOMUM EPICLITUM; RUMINANT STOMACH; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; STRONGYLOIDES; STRONGYLOIDES PAPILLOSUS;

ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; BUFFALOES; CATHEPSINS; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FASCIOLA; FASCIOLIASIS; FECES; HELMINTH PROTEINS; IMMUNOGLOBULIN G; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS;

ANIMALIA; BUBALUS; EXPLANATUM; FASCIOLA; FASCIOLA GIGANTICA; GASTROTHYLAX; PARAMPHISTOMUM EPICLITUM; STRONGYLOIDES PAPILLOSUS; VERMES;

EID: 83255185026     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2011.07.032     Document Type: Article

References (22)

1. Anuracpreeda P., Songkoomkrong S., Sethadavit M., Chotwiwatthanakun C., Tinikul Y., Sobhon P. Fasciola gigantica: production and characterization of a monoclonal antibody against recombinant cathepsin B3. Exp. Parasitol. 2011, 127:340-345.
2. Carnevale S., Rodriguez M.I., Guarnera E.A., Carmona C., Tanos T., Angel S.O. Immunodiagnosis of fasciolosis using recombinant procathepsin-L cysteine proteinase. Diag. Microbiol. Infect. Dis. 2001, 41:43-49.
3. Cordova M., Reategui L., Espinoza J.R. Immunodiagnosis of human fasciolosis with Fasciola hepatica cysteine proteinases. Trans. R. Soc. Trop. Med. Hyg. 1999, 93:54-57.
4. Cornelissen J.B.W.J., Gassenbeek C.P.H., Borgsteede F.H.M., Holland W.G., Harmsen M.M., Bocrsma W.J.A. Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin-L like protease. Int. J. Parasitol. 2001, 31:728-737.
5. Dalton J.P., Heffernan M. Thiol proteases released in vitro by Fasciola hepatica. Mol. Biochem. Parasitol. 1989, 35:161-166.
6. Dixit A.K., Yadav S.C., Sharma R.L. The 28kDa Fasciola gigantica cysteine proteinase in the diagnosis of prepatent ovine fasciolosis. Vet. Parasitol. 2002, 109:233-247.
7. Dixit A.K., Yadav S.C., Sharma R.L. Experimental bubaline fasciolosis: kinetics of antibody response using 28kDa Fasciola gigantica cysteine proteinase as antigen. Trop. Anim. Health Prod. 2004, 36:49-54.
8. Estuningsih E., Spithill T., Raadsma H., Law R., Adiwinata G., Meeusen E., Piedrafita D. Development and application of a faecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in Central Java, Indonesia. J. Parasitol. 2009, 95:450-455.
9. Fagbemi B.O., Guobadia E.E. Immunodiagnosis of fasciolosis in ruminants using 28kDa cysteine proteinase of Fasciola gigantica adult worms. Vet. Parasitol. 1995, 57:309-318.
10. Guobadia E.E., Fagbemi B.O. The isolation of Fasciola gigantica specific antigens and their use in the serodiagnosis of fasciolosis in sheep by the detection of circulating antigens. Vet. Parasitol. 1997, 68:269-282.
11. Gupta A., Dixit A.K., Dixit P., Mahajan C., Sharma R.L. Evaluation of dipstick-ELISA using 28kDa Fasciola gigantica cathepsin-L cysteine proteinase (FgCL3) for serodiagnosis of fasciolosis in naturally infected goats. Vet. Parasitol. 2011, 176:165-169.
12. Jefferies J.R., Campbell A.M., Van Rossum A.J., Barrett J., Brophy P.M. Proteomic analysis of Fasciola hepatica excretory-secretory products. Proteomics 2001, 1:1128-1132.
13. Jezek J., El Ridi R., Salah M., Wagih A., Aziz H.W., Tallima H., El Shafie M.H., Khalek T.A., Ammou F.F., Strongylis C., Moussis V., Tsikaris V. Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis. Biopolymers 2008, 90:349-357.
14. Mehra U.R., Dass R.S., Verma A.K., Sharma R.L., Yadav S.C. Effect of Fasciola gigantica infection on growth and nutrient utilization in buffalo calves. Vet. Rec. 1999, 145:699-702.
15. Morphew R.M., Wright H.A., LaCourse E.J., Woods D.J., Brophy P.M. Comparative proteomics of excretory-secretory proteins released by the liver fluke Fasciola hepatica in sheep host bile and during in vitro culture ex-host. Mol. Cell. Proteomic. 2007, 6:963-972.
16. O'Neill S.M., Parkinson M., Strauss W., Angles R., Dalton J.P. Immunodiagnosis of Fasciola hepatica infection (fasciolosis) in a human population inn the Bolivian Altiplano using purified cathepsin L cysteine proteinase. Am. J. Trop. Med. Hyg. 1998, 58:417-423.
17. Raina O.K., Yadav S.C., Sriveny D., Gupta S.C. Immunodiagnosis of bubaline fasciolosis with Fasciola gigantica cathepsin-L and recombinant cathepsin-L1-D proteases. Acta Trop. 2006, 98:145-151.
18. Smith A., Dowd A., McGonigle S.M., Keegan P., Brennan G., Trudgget A., Dalton J.P. Purification of cathepsin L like proteinase secreted by adult Fasciola hepatica. Mol. Biochem. Parasitol. 1993, 62:1-8.
19. Spithill T.W., Piedrafita D., Smooker P.M. Immunological approaches for the control of fasciolosis. Int. J. Parasitol. 1997, 27:1221-1235.
20. Sriveny D., Raina O.K., Yadav S.C., Chandra D., Jayraw A.K., Singh M., Velusamy R., Singh B.P. Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection. Vet. Parasitol. 2006, 135:25-31.
21. Yadav S.C., Saini M., Raina O.K., Nambi P.A., Jadav K., Sriveny D. Fasciola gigantica cathepsin-L cysteine proteinase in the detection of early experimental fasciolosis in ruminants. Parasitol. Res. 2005, 97:527-534.
22. Yamasaki H., Mineki R., Murayama K., Ito A., Aoki T. Characterization and expression of the Fasciola gigantica cathepsin-L gene. Int. J. Parasitol. 2002, 32:1031-1042.