The isolation of Fasciola gigantica-specific antigens and their use in the serodiagnosis of fasciolosis in sheep by the detection of circulating antigens

Veterinary Parasitology

Volumn 68, Issue 3, 1997, Pages 269-282

  Guobadia, Esther E ^a   Fagbemi, Benjamin O ^a  

^a UNIVERSITY OF IBADAN   (Nigeria)

Author keywords

circulating antigen; diagnosis Trematoda; ELISA; Fasciola gigantica; immunoblotting; SDS PAGE; sheep Trematoda

Indexed keywords

HORSERADISH PEROXIDASE; OXYCLOZANIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN DETECTION; ANTIGEN SPECIFICITY; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FASCIOLA; FASCIOLIASIS; NONHUMAN; SERODIAGNOSIS; SERUM; SHEEP; VETERINARY MEDICINE;

ANIMALS; ANTIBODIES, HELMINTH; ANTIBODY SPECIFICITY; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS, HELMINTH; ANTIPLATYHELMINTIC AGENTS; FASCIOLA; FASCIOLIASIS; IMMUNOBLOTTING; MOLECULAR WEIGHT; OXYCLOZANIDE; RABBITS; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SHEEP; SHEEP DISEASES; TIME FACTORS;

EID: 0031079787     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4017(96)01065-5     Document Type: Article

References (19)

1. Bradford, M.M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein using principles of protein dye binding. Ann. Biochem., 72: 248-254.
2. Chandrashekar, R., Ogunrinade, A.F., Akvarez, R.M., Kale, O.O. and Weil, G.J., 1990. Circulating immune complex associated parasite antigen in human onchocerciasis. J. Infect. Dis., 162: 1159-1164.
3. Craig, P.S. and Nelson, G.S., 1984. The detection of circulating antigen in human hydatid disease. Ann. Trop. Med. Parasitol., 48: 219-227.
4. Fagbemi, B.O. and Obarisiagbon, I.O., 1990. Comparative evaluation of the enzyme linked immuosorbent assay in the diagnosis of natural Fasciola gigantica infection in cattle. Vet. Q., 12: 35-38.
5. Fagbemi, B.O., Obarisiagbon, I.O. and Mbuh, J.V., 1995. Detection of circulating antigen in sera of Fasciola gigantica-infected cattle with antibodies reactive with a Fasciola-specific 88 kDa antigen. Vet. Parasitol., 58: 235-246.
6. Guobadia, E.E. and Fagbemi, B.O., 1995. Time-course analysis by EITB and ELISA of immune response of sheep infected with F. gigantica before and after chemotherapy. Vet. Parasitol., 58: 247-253.
7. Hillyer, G.V. and Galanes. M., 1988. Identification of a 17 kilodalton F. hepatica immunodiagnostic antigen by the enzyme immunoelectrotransfer blot techniques. J. Clin. Microbiol., 26: 2048-2053.
8. Langley, R.J. and Hillyer, G.V., 1989. Detection of circulating parasite antigen in fascioliasis by two-site enzyme-linked immunosorbent assays. Am. J. Trop. Med. Hyg., 41: 480-486.
9. Marrero, C.A., Santiago, N. and Hillyer, G.V., 1988. Evaluation of immunodiagnostic antigens in the excretory-secretory products of Fasciola hepatica. J. Parasitol., 74: 646-652.
10. Mitchell, G.F. and Anders, R.F., 1982. Parasitic antigens and their immunogenicity in infected hosts. In: M. Sela (Editor), The Antigens. Academic Press, New York, pp. 69-149.
11. Olaechea, F.V., Christensen, N.O. and Henriksen, S.A., 1990. A comparison of filtration, concentration and thick smear techniques in the diagnosis of Schistosoma bovis infection in cattle and sheep. Acta Trop., 47: 217-221.
12. Santiago, N. and Hillyer, G.V., 1988. Antibody profile by EITB and ELISA of cattle and sheep infected with F. hepatica. J. Parasitol., 74: 810-818.
13. Thienpoint, D., Rochette, F. and Vanparijs, O.F.J., 1979. Diagnosing Helminthiasis through Coprological Examination. Janssen Research Foundation, Beerse, Belgium, pp. 34-36.
14. Thorne, C.J.R., 1978. Techniques in Proteins and Enzyme Biochemistry, Part 1, Section B. Elsevier-North Holland, Amsterdam.
15. Voller, A. and De Savigny, D., 1982. Conjugate preparation. Enzyme linked immunosorbent assay (ELISA). In: N.R. Rose and H. Friedman (Editors), Manual of Clinical Immunology. American Society of Microbiology, Washington, DC, p. 506.
16. Weil, G.J., 1987. Dirofilaris immitis: identification and partial characterization of parasite antigens in the serum of infected dogs. Exp. Parasitol., 64: 244-251.
17. Weiss, N., 1986. Immunological approaches to the detection of prepatent onchocerciasis. J. Comp. Dis., 18: 254-260.
18. Zheng, H.-J., Tao Zheng-Hou, C.W. and Pessens, W.F., 1990. Comparison of dot-ELISA with sandwich-ELISA for the detection of circulating antigens in patients with Bancroftian filariasis. Am. J. Trop. Med. Hyg., 42: 546-549.
19. Zimmermann, G.L., Nelson, M.J. and Clark, C.R.B., 1985. Diagnosis of ovine fascioliasis by a dot enzyme linked immunosorbent assay, a rapid diagnostic technique. Am. J. Vet. Res., 46: 1513-1515.