Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis

Applied and Environmental Microbiology

Volumn 77, Issue 23, 2011, Pages 8249-8258

  Do, Thi Huyen ^a,c   Suzuki, Yuki ^b   Abe, Naoki ^b   Kaneko, Jun ^b   Itoh, Yoshifumi ^a,b   Kimura, Keitarou ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c Vietnamese Academy of Science and Technology   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOPHOSPHORYLATION; BACILLUS SUBTILIS; COMPLEX INTERACTION; ESCHERICHIA COLI CELLS; EXTRACELLULAR; IN-VITRO; MUTANT PROTEINS; PHOSPHATASE ACTIVITY; RECEPTOR KINASE; TWO COMPONENT SYSTEMS; WILD TYPES;

AMINO ACIDS; BACTERIOLOGY; ESCHERICHIA COLI; GENE ENCODING; PHOSPHORYLATION; PROTEINS;

BACILLI;

BACTERIAL PROTEIN; DEGQ PROTEIN, BACILLUS SP.; DEGS PROTEIN, BACTERIA; DEGU PROTEIN, BACTERIA; DRUG DERIVATIVE; HYBRID PROTEIN; PHOSPHATASE; POLY(GAMMA GLUTAMIC ACID); POLY(GAMMA-GLUTAMIC ACID); POLYGLUTAMIC ACID; TRANSACTIVATOR PROTEIN;

AMINO ACID; BACTERIUM; BIOASSAY; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; PEPTIDE; PROTEIN; RECOMBINATION;

ARTICLE; BACILLUS SUBTILIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENE INACTIVATION; GENE REPRESSION; GENETICS; METABOLISM; PHOSPHORYLATION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; PHOSPHORIC MONOESTER HYDROLASES; PHOSPHORYLATION; POLYGLUTAMIC ACID; RECOMBINANT FUSION PROTEINS; SUPPRESSION, GENETIC; TRANS-ACTIVATORS;

EID: 83255174926     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05827-11     Document Type: Article

References (36)

1. Amory, A., F. Kunst, E. Aubert, A. Klier, and G. Rapoport. 1987. Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J. Bacteriol. 169:324-333.
2. Ashikaga, S., H. Nanamiya, Y. Ohashi, and F. Kawamura. 2000. Natural genetic competence in Bacillus subtilis natto OK2. J. Bacteriol. 182:2411-2415.
3. Ashiuchi, M., K. Soda, and H. Misono. 1999. A poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336: gene cloning and biochemical analysis of poly-gamma-glutamate produced by Escherichia coli clone cells. Biochem. Biophys. Res. Commun. 263:6-12.
4. Candela, T., and A. Fouet. 2006. Poly-gamma-glutamate in bacteria. Mol. Microbiol. 60:1091-1098.
5. Dahl, M. K., T. Msadek, F. Kunst, and G. Rapoport. 1991. Mutational analysis of the Bacillus subtilis DegU regulator and its phosphorylation by the DegS protein kinase. J. Bacteriol. 173:2539-2547.
6. Dahl, M. K., T. Msadek, F. Kunst, and G. Rapoport. 1992. The phosphorylation state of the DegU response regulator acts as a molecular switch allowing either degradative enzyme synthesis or expression of genetic competence in Bacillus subtilis. J. Biol. Chem. 267:14509-14514.
7. Fujimoto, Z., I. Shiga, Y. Itoh, and K. Kimura. 2009. Crystallization and preliminary crystallographic analysis of poly-gamma-glutamate hydrolase from bacteriophage PhiNIT1. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65:913-916.
8. Guérout-Fleury, A. M., K. Shazand, N. Frandsen, and P. Stragier. 1995. Antibiotic-resistance cassettes for Bacillus subtilis. Gene 167:335-336.
9. Hamano, Y. (ed.). 2010. Microbiology monographs, vol. 15. Amino-acid homopolymers occurring in nature. Springer, New York, NY.
10. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
11. Jers, C., A. Kobir, E. O. Sondergaard, P. R. Jensen, and I. Mijakovic. 2011. Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain. PLoS One 6:e14653.
12. Kimura, K., and Y. Itoh. 2003. Characterization of poly-gamma-glutamate hydrolase encoded by a bacteriophage genome: possible role in phage infection of Bacillus subtilis encapsulated with poly-gamma-glutamate. Appl. Environ. Microbiol. 69:2491-2497.
13. Kimura, K., L. S. P. Tran, T. H. Do, and Y. Itoh. 2009. Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto). Biosci. Biotechnol. Biochem. 73:1149-1155.
14. Kimura, K., L. S. P. Tran, I. Uchida, and Y. Itoh. 2004. Characterization of Bacillus subtilis gamma-glutamyltransferase and its involvement in the degradation of capsule poly-gamma-glutamate. Microbiology 150:4115-4123.
15. Kobayashi, K. 2007. Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis. Mol. Microbiol. 66:395-409.
16. Kocianova, S., et al. 2005. Key role of poly-gamma-DL-glutamic acid in immune evasion and virulence of Staphylococcus epidermidis. J. Clin. Invest. 115:688-694.
17. Msadek, T., et al. 1990. Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU. J. Bacteriol. 172:824-834.
18. Msadek, T., F. Kunst, A. Klier, and G. Rapoport. 1991. DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ. J. Bacteriol. 173:2366-2377.
19. Mukai, K., M. Kawata-Mukai, and T. Tanaka. 1992. Stabilization of phosphorylated Bacillus subtilis DegU by DegR. J. Bacteriol. 174:7954-7962.
20. Mukai, K., M. Kawata, and T. Tanaka. 1990. Isolation and phosphorylation of the Bacillus subtilis degS and degU gene products. J. Biol. Chem. 265: 20000-20006.
21. Murray, E. J., T. B. Kiley, and N. R. Stanley-Wall. 2009. A pivotal role for the response regulator DegU in controlling multicellular behaviour. Microbiology 155:1-8.
22. Nagai, T., K. Koguchi, and Y. Itoh. 1997. Chemical analysis of poly-gammaglutamic acid produced by plasmid-free Bacillus subtilis (natto): evidence that plasmids are not involved in poly-gamma-glutamic acid production. J. Gen. Appl. Microbiol. 43:139-143.
23. Nicholson, W. L., and P. Setlow. 1990. Sporulation, germination and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, West Sussex, United Kingdom.
24. Nishito, Y., et al. 2010. Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data. BMC Genomics 11:243.
25. Ogura, M., K. Shimane, K. Asai, N. Ogasawara, and T. Tanaka. 2003. Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis. Mol. Microbiol. 49:1685-1697.
26. Ohsawa, T., K. Tsukahara, and M. Ogura. 2009. Bacillus subtilis response regulator DegU is a direct activator of pgsB transcription involved in gammapoly-glutamic acid synthesis. Biosci. Biotechnol. Biochem. 73:2096-2102.
27. Osera, C., G. Amati, C. Calvio, and A. Galizzi. 2009. SwrAA activates poly-gamma-glutamate synthesis in addition to swarming in Bacillus subtilis. Microbiology 155:2282-2287.
28. Shih, I. L., and Y. T. Van. 2001. The production of poly-(gamma-glutamic acid) from microorganisms and its various applications. Bioresour. Technol. 79:207-225.
29. Shimane, K., and M. Ogura. 2004. Mutational analysis of the helix-turn-helix region of Bacillus subtilis response regulator DegU, and identification of cis-acting sequences for DegU in the aprE and comK promoters. J. Biochem. 136:387-397.
30. Stanley, N. R., and B. A. Lazazzera. 2005. Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-DL-glutamic acid production and biofilm formation. Mol. Microbiol. 57: 1143-1158.
31. Tanaka, T., M. Kawata, and K. Mukai. 1991. Altered phosphorylation of Bacillus subtilis DegU caused by single amino acid changes in DegS. J. Bacteriol. 173:5507-5515.
32. Tran, L. S., T. Nagai, and Y. Itoh. 2000. Divergent structure of the ComQXPA quorum-sensing components: molecular basis of strain-specific communication mechanism in Bacillus subtilis. Mol. Microbiol. 37:1159-1171.
33. Tsuge, K., T. Ano, M. Hirai, Y. Nakamura, and M. Shoda. 1999. The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production. Antimicrob. Agents Chemother. 43:2183-2192.
34. Tsukahara, K., and M. Ogura. 2008. Characterization of DegU-dependent expression of bpr in Bacillus subtilis. FEMS Microbiol. Lett. 280:8-13.
35. Urushibata, Y., S. Tokuyama, and Y. Tahara. 2002. Characterization of the Bacillus subtilis ywsC gene, involved in gamma-polyglutamic acid production. J. Bacteriol. 184:337-343.
36. Yoshikawa, T., et al. 2008. Development of amphiphilic gamma-PGA-nanoparticle based tumor vaccine: potential of the nanoparticulate cytosolic protein delivery carrier. Biochem. Biophys. Res. Commun. 366:408-413.